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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1308 [new locus tag: SA_RS07400 ]
- pan locus tag?: SAUPAN003937000
- symbol: rpsA
- pan gene symbol?: rpsA
- synonym:
- product: 30S ribosomal protein S1
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1308 [new locus tag: SA_RS07400 ]
- symbol: rpsA
- product: 30S ribosomal protein S1
- replicon: chromosome
- strand: -
- coordinates: 1513700..1514875
- length: 1176
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124147 NCBI
- RefSeq: NP_374590 NCBI
- BioCyc: see SA_RS07400
- MicrobesOnline: 103616 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGACTGAAGAATTCAATGAATCAATGATTAACGATATTAAAGAAGGTGACAAAGTCACT
GGCGAGGTACAACAAGTTGAAGACAAGCAAGTTGTTGTTCATATCAACGGTGGTAAATTT
AATGGGATTATTCCTATTAGTCAACTATCTACGCATCATATTGATAGCCCAAGTGAAGTT
GTAAAAGAGGGCGACGAAGTTGAAGCATATGTCACTAAAGTTGAGTTTGATGAAGAAAAT
GAAACTGGAGCTTACATCTTATCTAGAAGACAACTTGAAACTGAGAAGTCTTATAGTTAT
TTACAAGAAAAATTAGATAATAATGAAATCATCGAAGCGAAAGTAACAGAAGTAGTTAAA
GGTGGTTTGGTTGTTGATGTAGGACAAAGAGGTTTTGTTCCGGCTTCACTAATTTCAACA
GACTTCATTGAGGATTTCTCTGTGTTTGATGGACAAACAATTCGTATTAAAGTTGAAGAA
TTGGATCCTGAAAATAATAGAGTCATTTTAAGCCGTAAAGCAGTTGAACAAGAAGAAAAC
GATGCTAAAAAAGATCAATTATTACAATCTTTAAATGAAGGCGATGTTATTCATGGTAAA
GTAGCGCGTTTAACTCAATTTGGTGCATTTATAGACATTGGTGGTGTTGATGGTTTAGTG
CATGTATCTGAACTTTCTCACGAACATGTTCAAACACCAGAAGAAGTAGTTTCAATTGGT
CAAGATGTTAAAGTTAAAATTAAATCTATTGATAGAGATACAGAACGTATTTCATTATCA
ATCAAAGATACGTTACCAACACCTTTCGAAAATATTAAAGGTCAATTCCACGAAAATGAT
GTCATTGAAGGTGTCGTAGTAAGATTGGCAAACTTTGGTGCATTTGTTGAAATTGCACCA
GGTGTACAAGGACTTGTACATATTTCTGAAATTGCACACAAACACATTGGTACGCCAGGT
GAAGTGTTAGAACCTGGTCAACAAGTAAATGTTAAAATATTAGGTATTGATGAAGAGAAT
GAAAGAGTATCACTATCTATTAAAGCAACATTACCAAACGAAGATGTTGTTGAAAGTGAT
CCTTCTACGACTAAGGCGTACTTAGAAAACGAAGAAGAAGATAATCCAACAATTGGCGAT
ATGATTGGTGATAAACTTAAAAATCTTAAACTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1308 [new locus tag: SA_RS07400 ]
- symbol: RpsA
- description: 30S ribosomal protein S1
- length: 391
- theoretical pI: 4.27604
- theoretical MW: 43309
- GRAVY: -0.38977
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 286.7)and 8 moreTranscription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 147.6)guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 59.4)Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 44)Transcription Degradation of RNA ribonuclease R (TIGR02063; EC 3.1.-.-; HMM-score: 37)MAST domain (TIGR04204; HMM-score: 14.1)Energy metabolism Glycolysis/gluconeogenesis carbon storage regulator (TIGR00202; HMM-score: 9.8)Regulatory functions RNA interactions carbon storage regulator (TIGR00202; HMM-score: 9.8)Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 9.6)
- TheSEED :
- SSU ribosomal protein S1p
- PFAM: OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 251)and 8 moreCusF_Ec; Copper binding periplasmic protein CusF (PF11604; HMM-score: 17.1)Cdc48_2-like (CL0402) CDC48_2; Cell division protein 48 (CDC48), domain 2 (PF02933; HMM-score: 16)OB (CL0021) S1_2; S1 domain (PF13509; HMM-score: 15.6)EXOSC1; Exosome component EXOSC1/CSL4 (PF10447; HMM-score: 13.4)RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 11.5)no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 9.9)CsrA; Global regulator protein family (PF02599; HMM-score: 7.9)KOW (CL0107) KOW; KOW motif (PF00467; HMM-score: 7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.021822
- TAT(Tat/SPI): 0.000535
- LIPO(Sec/SPII): 0.000886
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGIIPISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQEKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEELDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIHGKVARLTQFGAFIDIGGVDGLVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQFHENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKILGIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)