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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1416 [new locus tag: SA_RS07995 ]
  • pan locus tag?: SAUPAN004174000
  • symbol: SA1416
  • pan gene symbol?: comEC
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1416 [new locus tag: SA_RS07995 ]
  • symbol: SA1416
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1622337..1624538
  • length: 2202
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTC
    TCTACATTTCTTTTCATTTTACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTAT
    GCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGTATTTACATTATTCACAACAA
    GCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
    GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAAT
    GAAATATATCCTTTCTTTTTAACAGATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGT
    CGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGAAAATAAGTTTGTAACTCTTAAA
    TTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
    CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATT
    ATGGCATTGATTACTGGTGACGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAA
    GAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGCATATAGCTGCAATTGTATTC
    TTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
    ATCATTGTATTAACTTTATATGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCT
    ATAATAATGACAACTCTTGTACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAG
    CTATTAGCATTTGCATTTATAATTATGTTTATTTTAAACCCACTAGTTGTTTATGATATT
    GGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
    CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTT
    ATCGTTGCCATTCCAAACTTTCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATT
    TTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTATTTTATTCTTTATTACAAGT
    CATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
    GACTGGTTACTAGACCTATTCACAAGAATTAAGCAATCACATTTTTCTGTTCCCAAGTTT
    AATGATTGGATATTTATAATATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCT
    AAACGTAAATATATATTGGTTACGTTTTGGACTATAATTATTCTGACATTATTAATAACG
    TTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
    TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGAT
    GATACTAAACAACCTAGTTATTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAA
    AGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATCCACACAATGACCATATTGGT
    GAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
    TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTT
    ATGGATGTAAGACAAGTTAGTAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGAT
    AGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGATTATTACTATGATTACATAT
    CAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
    AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACA
    AGTAGTTCTAAAGAATTTATAGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGG
    AAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTAAACGATTGCAAAGGATTCGC
    AGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
    AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2202

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1416 [new locus tag: SA_RS07995 ]
  • symbol: SA1416
  • description: hypothetical protein
  • length: 733
  • theoretical pI: 10.0037
  • theoretical MW: 84997.4
  • GRAVY: 0.239018

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes DNA transformation DNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM-score: 277.4)
    and 3 more
    Unknown function General ComEC/Rec2-related protein (TIGR00360; HMM-score: 79.2)
    Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 17.1)
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 15.7)
  • TheSEED  :
    • Late competence protein ComEC, DNA transport
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEC, DNA transport
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEC, DNA transport
  • PFAM:
    no clan defined Competence; Competence protein (PF03772; HMM-score: 182.2)
    and 3 more
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 80.3)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 13.9)
    no clan defined DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002734
    • TAT(Tat/SPI): 0.00042
    • LIPO(Sec/SPII): 0.037329
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEKHKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGITIIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigH* regulon
    SigH*(sigma factor)controlling competence and phage integrase genes;  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kazuya Morikawa, Yumiko Inose, Hideyuki Okamura, Atsushi Maruyama, Hideo Hayashi, Kunio Takeyasu, Toshiko Ohta
    A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes.
    Genes Cells: 2003, 8(8);699-712
    [PubMed:12875655] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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