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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1483 [new locus tag: SA_RS08345 ]
  • pan locus tag?: SAUPAN004270000
  • symbol: tnpB
  • pan gene symbol?: tnpB
  • synonym:
  • product: transposase B for Tn554

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1483 [new locus tag: SA_RS08345 ]
  • symbol: tnpB
  • product: transposase B for Tn554
  • replicon: chromosome
  • strand: -
  • coordinates: 1688038..1689930
  • length: 1893
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAATGCTTCTAGTAAAAGGAAAATTATTAGTCAGAGTGAGATTAGCAAAAAAATAGCT
    GTAATGAATGAAGAAATGCAGGGGTTTTGGGCTAATAATAGTTGGGATATAAGAAAATGT
    CCACATCCTTCTGCCATAGAATTAAGTAAGAATCCTGCTTTAAGGAATCGTTGGGTTCGT
    TTTGAACGTGTTAAAAATCTGTGGTTAAGAACAGAATTGAAATATTTTTATTTTTACCAT
    TTAAACAATGGAATATGGAATGCAAAAACTGTCTGGATTAGAAAAGGAACAGTAATTAAT
    AAAATGTTAGATTTCTTAGATTTAAAGTATCCTAGCATTACTTCAATTACTGAAGTTCCT
    ATTGAAAAAGCAATGACGGAGTATAGAACTTATTTGACAAAACGGGGTGTTAGAATTACC
    ACTACTAATTATAAGATTACTGCTAATCAAGAAAAAACACCTGTAAAAGCTAATTCCTAC
    TATGTTACTAATCTAAAACAATTTATGGAGTTTTATGAGAACTTTTATTTTGATGGAGAG
    GAGTGGGATAAAGACGTTTGGGATAGACGTAACTTACCTTTGCCAGATGATAAGGTTAAC
    CCAACACAATATGAATATACAATTAACTTTAAAGGGTTTCGGAATACATATTTTAAACAA
    CTTGTAAAAAGATATTGTAAGTTGAGATTGAACGTGGATAGCTTTTCCTATGTAAGTGAT
    ATTGCCCAAAGACTTAAAGAGTTCTTTAATTTTCTGGACATGAAATTTAAACAAGTTCAG
    AGAGTACACCAATTAACGAGAGTGGAAATTGAAGCATATTTAAGTGAACTAAACATGATG
    GGAATAAAACCTAGTACAATAACTGGGAGGATCTCTATATTGGAAGGACTATTTAGTACC
    CTTCTTAGGCTAGAATGGGATGATGTTCCTTCCAAAATATTAATTTATTCTGAGGACTAT
    CCGAAAATACCAAGAGCAAAACCACGCTTTATAGATGAATTCGTCCTAGAGCAATTGAAC
    AGTCATCTTGATAAATTACCCGAATATATAGCTACGATGACTATGATTGTTCAAGAATGT
    GGAATGAGGATAAGTGAATTGTGCACCTTGAAAAAAGGCTGTCTATTAGAGGACAAAGAT
    GGAGATTTCTTTTTAAAGTATTATCAATGGAAAATGAAAAAGGAGCATATAGTTCCAATA
    TCTAAAGAGGTAGCTTTACTTATTAAAGTTCGGGAAGATAAAGTTTCAGAGGAATTTCCA
    GATAGTGAATACCTCTTTCCAAGAAAAGATGGATCGCCATTAAAACAAGAAACATTTAGA
    GGTGAGTTAAATAAATTAGCTTATGAGCAAAATATAGTGGATAAATCAGGTGAGATTTAT
    AGATTCCATGCCCATGCCTTTCGCCATACAGTAGGAACAAGAATGATTAACAACGGGATG
    CCCCAGCATATTGTGCAGAAATTTTTGGGGCATGAAAGCCCAGAAATGACAAGCAGATAC
    GCTCATATCTTTGATGAAACTCTAAAAAATGAATTTACTAAATTTCAGGAAAAACTGGTT
    ACCAATAATGGAGATGTGCTTGATCTAGATGAAGATAATGAAGTCGATGATGTAGAGCTT
    CAATGGTTCAAGAAAAATATAAATGCACAAGTGCTTCCAAATGGTTATTGTAGATTGCCA
    GTAGTAGCAGGTGGTTGTCCACATGCGAATGCATGCTTAGATTGCACTCACTTCTGTACC
    AGTAAGCAATTCTTACCACAGCACGAAGAACAGTTAGAGCGTACAGAAGAGTTATTAGCC
    ATAGCTAAGGATAAACAATGGCAAAGACAAGTAGAGACTAATAGCCGTGTTAAAGAGCGT
    TTAGAACAAATCATTGGAAGTTTGACGGGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1893

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1483 [new locus tag: SA_RS08345 ]
  • symbol: TnpB
  • description: transposase B for Tn554
  • length: 630
  • theoretical pI: 8.35267
  • theoretical MW: 74438.9
  • GRAVY: -0.568889

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 95.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 77.8)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 29.9)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 29.9)
  • TheSEED  :
    • Transposase B from transposon Tn554
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 151.8)
    and 4 more
    l-integrase_N (CL0469) Phage_int_SAM_4; Phage integrase, N-terminal SAM-like domain (PF13495; HMM-score: 27.6)
    Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 21.6)
    DNA-mend (CL0382) Phage_integr_3; Archaeal phage integrase (PF16795; HMM-score: 17.5)
    l-integrase_N (CL0469) Phage_int_SAM_2; Phage integrase, N-terminal (PF12834; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011768
    • TAT(Tat/SPI): 0.001674
    • LIPO(Sec/SPII): 0.001624
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)

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