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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1489 [new locus tag: SA_RS08380 ]
- pan locus tag?: SAUPAN004277000
- symbol: tag
- pan gene symbol?: tag
- synonym:
- product: DNA-3-methyladenine glycosidase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1489 [new locus tag: SA_RS08380 ]
- symbol: tag
- product: DNA-3-methyladenine glycosidase
- replicon: chromosome
- strand: +
- coordinates: 1696976..1697536
- length: 561
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124333 NCBI
- RefSeq: NP_374776 NCBI
- BioCyc: see SA_RS08380
- MicrobesOnline: 103802 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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541ATGAATGAATGCGCATTTGGTACTAAAGATCCAGTCTACTTAAACTATCATGATCATGTA
TGGGGACAACCGCTCTATGATAGCAAGGCATTGTTTAAACTTTTAGCATTAGAATCACAA
CATGCTGGGCTATCTTGGTTAACTATTTTAAAAAAGAAAGAAGCCTATGAAGAAGCATTT
TATGATTTCGAACCAGAAAAGGTAGCACAAATGACCGCTCAAGATATCGACCGCTTAATG
ACTTTTCCAAATATCGTTCATCATCGTAAAAAATTAGAAGCAATTGTTAATCAAGCTCAA
GGGTATTTAAAAATTGAACAAGCATATGGTAGTTTTAGTAAATTTTTATGGTCTTATGTA
AATGGTAAGCCTAAAGATTTGCAGTATGAACATGCTTCTGATCGTATCACAGTTGATGAT
ACTGCAACACAACTATCTAAAGATTTAAAACAATACGGGTTTAAATTTTTAGGTCCAGTA
ACAGTATTTTCGTTTTTAGAAGCAGCTGGTTTATATGATGCACATTTAAAAGATTGTCCA
TCAAAGCCTAAACACAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1489 [new locus tag: SA_RS08380 ]
- symbol: Tag
- description: DNA-3-methyladenine glycosidase
- length: 186
- theoretical pI: 6.95931
- theoretical MW: 21415.2
- GRAVY: -0.479032
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair DNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 189.1)
- TheSEED :
- DNA-3-methyladenine glycosylase (EC 3.2.2.20)
- PFAM: no clan defined Adenine_glyco; Methyladenine glycosylase (PF03352; HMM-score: 214.9)and 2 moreCOOH-NH2_lig; Phage phiEco32-like COOH.NH2 ligase-type 2 (PF14395; HMM-score: 11.7)DUF3326; Protein of unknown function (DUF3326) (PF11805; HMM-score: 10.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002479
- TAT(Tat/SPI): 0.000182
- LIPO(Sec/SPII): 0.000511
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLKDCPSKPKHN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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