From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1489 [new locus tag: SA_RS08380 ]
  • pan locus tag?: SAUPAN004277000
  • symbol: tag
  • pan gene symbol?: tag
  • synonym:
  • product: DNA-3-methyladenine glycosidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1489 [new locus tag: SA_RS08380 ]
  • symbol: tag
  • product: DNA-3-methyladenine glycosidase
  • replicon: chromosome
  • strand: +
  • coordinates: 1696976..1697536
  • length: 561
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATGAATGCGCATTTGGTACTAAAGATCCAGTCTACTTAAACTATCATGATCATGTA
    TGGGGACAACCGCTCTATGATAGCAAGGCATTGTTTAAACTTTTAGCATTAGAATCACAA
    CATGCTGGGCTATCTTGGTTAACTATTTTAAAAAAGAAAGAAGCCTATGAAGAAGCATTT
    TATGATTTCGAACCAGAAAAGGTAGCACAAATGACCGCTCAAGATATCGACCGCTTAATG
    ACTTTTCCAAATATCGTTCATCATCGTAAAAAATTAGAAGCAATTGTTAATCAAGCTCAA
    GGGTATTTAAAAATTGAACAAGCATATGGTAGTTTTAGTAAATTTTTATGGTCTTATGTA
    AATGGTAAGCCTAAAGATTTGCAGTATGAACATGCTTCTGATCGTATCACAGTTGATGAT
    ACTGCAACACAACTATCTAAAGATTTAAAACAATACGGGTTTAAATTTTTAGGTCCAGTA
    ACAGTATTTTCGTTTTTAGAAGCAGCTGGTTTATATGATGCACATTTAAAAGATTGTCCA
    TCAAAGCCTAAACACAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    561

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1489 [new locus tag: SA_RS08380 ]
  • symbol: Tag
  • description: DNA-3-methyladenine glycosidase
  • length: 186
  • theoretical pI: 6.95931
  • theoretical MW: 21415.2
  • GRAVY: -0.479032

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 189.1)
  • TheSEED  :
    • DNA-3-methyladenine glycosylase (EC 3.2.2.20)
    DNA Metabolism DNA repair DNA Repair Base Excision  DNA-3-methyladenine glycosylase (EC 3.2.2.20)
  • PFAM:
    no clan defined Adenine_glyco; Methyladenine glycosylase (PF03352; HMM-score: 214.9)
    and 2 more
    COOH-NH2_lig; Phage phiEco32-like COOH.NH2 ligase-type 2 (PF14395; HMM-score: 11.7)
    DUF3326; Protein of unknown function (DUF3326) (PF11805; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002479
    • TAT(Tat/SPI): 0.000182
    • LIPO(Sec/SPII): 0.000511
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGLSWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGYLKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPVTVFSFLEAAGLYDAHLKDCPSKPKHN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA1489&oldid=94038"