PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1535 [new locus tag: SA_RS08640 ]
• pan locus tag^?: SAUPAN004365000
• symbol: tpx
• pan gene symbol^?: tpx
• synonym:
• product: thiol peroxidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1535 [new locus tag: SA_RS08640 ]
• symbol: tpx
• product: thiol peroxidase
• replicon: chromosome
• strand: -
• coordinates: 1754240..1754734
• length: 495
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124380 NCBI
• RefSeq: NP_374823 NCBI
• BioCyc: see SA_RS08640
• MicrobesOnline: 103849 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
  
  ATGACTGAAATAACATTCAAAGGTGGACCAATCCACTTAAAAGGTCAACAAATTAATGAA
  GGTGATTTTGCACCTGATTTTACAGTGTTAGATAATGACTTAAATCAAGTAACATTAGCA
  GATTATGCTGGTAAAAAGAAATTAATTAGTGTGGTACCATCAATTGATACAGGTGTTTGT
  GATCAGCAGACTCGCAAATTCAACTCTGAAGCTTCTAAAGAAGAGGGGATTGTGCTTACA
  ATTTCAGCAGACTTACCATTCGCACAAAAAAGATGGTGCGCTTCAGCAGGTTTAGACAAT
  GTCATTACATTAAGTGACCACCGTGACTTATCATTTGGTGAAAACTATGGCGTTGTTATG
  GAAGAACTTCGCTTATTAGCTCGTGCAGTATTTGTATTAGATGTAGATAATAAAGTTGTT
  TATAAAGAAATCGTTAGTGAAGGTACTGATTTCCCAGATTTTGATGCTGCTTTAGCTGCA
  TACAAAAATATTTAA
  60
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  495
  

⊟Protein

⊟General

• locus tag: SA1535 [new locus tag: SA_RS08640 ]
• symbol: Tpx
• description: thiol peroxidase
• length: 164
• theoretical pI: 4.34422
• theoretical MW: 18047.3
• GRAVY: -0.119512

⊟Function

• reaction:
  EC 1.11.1.15?  ExPASy
  Peroxiredoxin 2 R'-SH + ROOH = R'-S-S-R' + H₂O + ROH
• TIGRFAM:
  Cellular processes Cellular processes Detoxification peroxiredoxin (TIGR03137; EC 1.11.1.15; HMM-score: 24.9)
  Cellular processes Cellular processes Adaptations to atypical conditions peroxiredoxin (TIGR03137; EC 1.11.1.15; HMM-score: 24.9)
• TheSEED  :
  • Thiol peroxidase Tpx (EC 1.11.1.24)
  Sulfur Metabolism Sulfur Metabolism - no subcategory Thioredoxin-disulfide reductase  Thiol peroxidase, Tpx-type (EC 1.11.1.15)
• PFAM:
  Thioredoxin (CL0172) Redoxin; Redoxin (PF08534; HMM-score: 88.8)
  and 1 more
  AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 63.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003082
  • TAT(Tat/SPI): 0.000174
  • LIPO(Sec/SPII): 0.000481
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927290 NCBI
• RefSeq: NP_374823 NCBI
• UniProt: P99146 UniProt

⊟Protein sequence

• MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAGKKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVITLSDHRDLSFGENYGVVMEELRLLARAVFVLDVDNKVVYKEIVSEGTDFPDFDAALAAYKNI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ackA < SA1534 < tpx

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)