PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

Views

• Read
• Edit
• History

More

• Edit source

⊟Summary

• organism: Staphylococcus aureus N315
locus tag: SA1756
pan locus tag^?: SAUPAN005024000
symbol: SA1756
pan gene symbol^?: —
• synonym:
• product: truncated amidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1756
• symbol: SA1756
• product: truncated amidase
• replicon: chromosome
• strand: -
• coordinates: 2008105..2008311
• length: 207
• essential: no DEG

⊟Accession numbers

• Gene ID: 3251083 NCBI
• RefSeq: NP_375051 NCBI
• BioCyc:
• MicrobesOnline: 104077 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  
  GTGAGGGATGGCTATTCAACTAATTCAAGAATTACAGGTGTATTACCCAATAACGCAACG
  ATCAAATATGACGGCGCATATTGCATTAATGGCTATAGATGGATTACTTATATTGCTAAT
  AGTGGACAACGTCGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGC
  GGTTTTGGTAAGTTTAGTGCAGTTTGA
  60
  120
  180
  207
  

⊟Protein

⊟General

• locus tag: SA1756
• symbol: SA1756
• description: truncated amidase
• length: 68
• theoretical pI: 9.99622
• theoretical MW: 7501.34
• GRAVY: -0.439706

⊟Function

• TIGRFAM:
• TheSEED  :
  • Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
• ⊞PFAM:
  SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 35.8)
  and 1 more
  SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 18.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.9998
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.115141
  • TAT(Tat/SPI): 0.000989
  • LIPO(Sec/SPII): 0.002763
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927518 NCBI
• RefSeq: NP_375051 NCBI
• UniProt: A0A0H3JQW5 UniProt

⊟Protein sequence

• MRDGYSTNSRITGVLPNNATIKYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISGFGKFSAV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1756 < SA1757

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications