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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1874 [new locus tag: SA_RS10785 ]
  • pan locus tag?: SAUPAN005341000
  • symbol: alr
  • pan gene symbol?: alr
  • synonym:
  • product: alanine racemase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1874 [new locus tag: SA_RS10785 ]
  • symbol: alr
  • product: alanine racemase
  • replicon: chromosome
  • strand: -
  • coordinates: 2121786..2122934
  • length: 1149
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTCGGATAAATATTATAGATCTGCGTATATGAATGTAGATTTAAACGCTGTTGCATCA
    AATTTCAAAGTATTCAGTACATTGCATCCAAATAAAACAGTGATGGCTGTCGTTAAAGCC
    AATGCCTATGGACTAGGTAGTGTTAAAGTAGCACGTCATTTAATGGAAAATGGCGCCACA
    TTTTTTGCTGTAGCAACGTTAGATGAAGCGATAGAACTTAGAATGCATGGGATTACTGCT
    AAAATTTTAGTCTTAGGTGTGTTACCAGCTAAAGATATTGATAAAGCGATACAACACAGA
    GTTGCCTTAACGGTTCCGTCTAAACAGTGGTTGAAAGAAGCAATTAAAAACATTTCTGGT
    GAGCAAGAGAAAAAGTTATGGTTGCACATTAAATTAGATACAGGAATGGGACGTTTAGGT
    ATTAAAGATACTAATACGTATCAAGAAGTGATTGAAATCATTCAACAATATGAGCAACTT
    GTATTTGAAGGCGTGTTTACACACTTTGCCTGTGCTGACGAACCAGGAGATATGACAACT
    GAACAATATCAACGTTTTAAAGATATGGTCAATGAAGCAATTAAACCTGAATATATACAT
    TGTCAGAACTCAGCAGGCTCTCTATTAATGGATTGCCAATTCTGTAATGCAATAAGACCA
    GGAATTTCCCTTTATGGATATTATCCATCAGAGTATGTACAGCAAAAAGTTAAAGTACAC
    CTTAAACCAAGTGTGCAATTAATTGCTAATGTAGTTCAAACAAAGACGCTACAAGCGGGT
    GAGTCTGTAAGTTATGGTGCAACTTATACAGCTACTGACCCAACTACAATAGCTTTGTTA
    CCTATTGGATATGCAGATGGCTATTTACGCATAATGCAAGGTAGCTTTGTAAATGTAAAT
    GGTCATCAATGCGAAGTTATTGGTCGCGTATGTATGGATCAGACAATTGTTAAAGTGCCA
    GATCAAGTTAAAGCTGGAGATTCGGTGATTTTAATAGATAATCATAGAGAAAGTCCACAG
    TCGGTAGAGGTGGTAGCTGAAAAGCAACATACTATTAATTATGAAGTGCTTTGTAACTTG
    TCGAGACGTTTGCCGCGAATCTATCATGATGGTGATCAACGTTTTGTAACAAATGAATTG
    TTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1149

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1874 [new locus tag: SA_RS10785 ]
  • symbol: Alr
  • description: alanine racemase
  • length: 382
  • theoretical pI: 7.1235
  • theoretical MW: 42808.9
  • GRAVY: -0.15733

Function[edit | edit source]

  • reaction:
    EC 5.1.1.1?  ExPASy
    Alanine racemase L-alanine = D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 372.3)
    and 1 more
    PRTRC system protein B (TIGR03737; HMM-score: 12.1)
  • TheSEED:  
    Amino Acids and Derivatives Alanine, serine, and glycine Alanine biosynthesis  Alanine racemase (EC 5.1.1.1)
    and 1 more
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  Alanine racemase (EC 5.1.1.1)
  • PFAM:
    TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 223.1)
    and 2 more
    no clan defined Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 143.4)
    Styelin; Styelin A-E (PF17562; HMM-score: 13.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014203
    • TAT(Tat/SPI): 0.002283
    • LIPO(Sec/SPII): 0.000643
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]