PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1949 [new locus tag: SA_RS11190 ]
• pan locus tag^?: SAUPAN005448000
• symbol: truncated-SA
• pan gene symbol^?: truncated-SA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1949 [new locus tag: SA_RS11190 ]
• symbol: truncated-SA
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2198308..2198781
• length: 474
• essential: no DEG

⊟Accession numbers

• Gene ID: 1124850 NCBI
• RefSeq: NP_375254 NCBI
• BioCyc: see SA_RS11190
• MicrobesOnline: 104280 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGTTTACTTACTTTAAATCAGCATTTAAAAATGCTAAACCACAATTATTAATAACATTG
  ATATATGCACTTATAGCATTTGCTGTAATTGCAGTTGTATATTTACTTGCTAATTTCCAG
  CTTGCTAAGTATGCACAAACTATTGCAATTTATTCACAATTTGGGCAAAAACCACCAGTA
  GATGCATATTTAAAAGTTATCGCTGTATTACTTATAGCAGCTGTAGTTTCACTGTTTGTT
  TTAGTTCAAATTTTTATCGGAATTACAAATGTTATGAAACGAGCAATGAGTCATGAAAAA
  GTAAAATTTACCGATTTATTTATTGCATTTAAAAAAGGTAATTATTTAAAGAGCGTTCTG
  ATCGGTCTTGTATCTATAGCAATGATTATCGTTCTTTCACTTCTGACATCATTACTATAC
  AAATTATTTTCTCCAGTATCAGAAATGATTATGAATACATCTTATCAAGAGTAA
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  474
  

⊟Protein

⊟General

• locus tag: SA1949 [new locus tag: SA_RS11190 ]
• symbol: Truncated-SA
• description: hypothetical protein
• length: 157
• theoretical pI: 10.2391
• theoretical MW: 17574.2
• GRAVY: 0.965605

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 9.5)
  TIGR03943 family protein (TIGR03943; HMM-score: 8.1)
• TheSEED  :
  • Lytic regulatory protein
• PFAM:
  no clan defined Sulf_transp; Sulphur transport (PF04143; HMM-score: 15.2)
  Abhydrolase_9_N; Alpha/beta-hydrolase family N-terminus (PF15420; HMM-score: 13)
  DUF624; Protein of unknown function, DUF624 (PF04854; HMM-score: 12.9)
  Yip1 (CL0112) Yip1; Yip1 domain (PF04893; HMM-score: 12.3)
  and 6 more
  no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 6.7)
  Flp_Fap; Flp/Fap pilin component (PF04964; HMM-score: 6.4)
  NPCC; Nuclear pore complex component (PF08058; HMM-score: 6.3)
  DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 6.2)
  DUF4307; Domain of unknown function (DUF4307) (PF14155; HMM-score: 6.1)
  DUF2975; Protein of unknown function (DUF2975) (PF11188; HMM-score: 6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016051
  • TAT(Tat/SPI): 0.000434
  • LIPO(Sec/SPII): 0.00222
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 15927721 NCBI
• RefSeq: NP_375254 NCBI
• UniProt: A0A0H3JMZ0 UniProt

⊟Protein sequence

• MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKYAQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVKFTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNTSYQE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications