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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1954 [new locus tag: SA_RS11215 ]
- pan locus tag?: SAUPAN005453000
- symbol: tnpB
- pan gene symbol?: tnpB
- synonym:
- product: transposition regulatory protein tnpB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1954 [new locus tag: SA_RS11215 ]
- symbol: tnpB
- product: transposition regulatory protein tnpB
- replicon: chromosome
- strand: -
- coordinates: 2202367..2204259
- length: 1893
- essential: no DEG
⊟Accession numbers[edit | edit source]
- Gene ID: 1124855 NCBI
- RefSeq: NP_375259 NCBI
- BioCyc: see SA_RS11215
- MicrobesOnline: 104285 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1861ATGAATGCTTCTAGTAAAAGGAAAATTATTAGTCAGAGTGAGATTAGCAAAAAAATAGCT
GTAATGAATGAAGAAATGCAGGGGTTTTGGGCTAATAATAGTTGGGATATAAGAAAATGT
CCACATCCTTCTGCCATAGAATTAAGTAAGAATCCTGCTTTAAGGAATCGTTGGGTTCGT
TTTGAACGTGTTAAAAATCTGTGGTTAAGAACAGAATTGAAATATTTTTATTTTTACCAT
TTAAACAATGGAATATGGAATGCAAAAACTGTCTGGATTAGAAAAGGAACAGTAATTAAT
AAAATGTTAGATTTCTTAGATTTAAAGTATCCTAGCATTACTTCAATTACTGAAGTTCCT
ATTGAAAAAGCAATGACGGAGTATAGAACTTATTTGACAAAACGGGGTGTTAGAATTACC
ACTACTAATTATAAGATTACTGCTAATCAAGAAAAAACACCTGTAAAAGCTAATTCCTAC
TATGTTACTAATCTAAAACAATTTATGGAGTTTTATGAGAACTTTTATTTTGATGGAGAG
GAGTGGGATAAAGACGTTTGGGATAGACGTAACTTACCTTTGCCAGATGATAAGGTTAAC
CCAACACAATATGAATATACAATTAACTTTAAAGGGTTTCGGAATACATATTTTAAACAA
CTTGTAAAAAGATATTGTAAGTTGAGATTGAACGTGGATAGCTTTTCCTATGTAAGTGAT
ATTGCCCAAAGACTTAAAGAGTTCTTTAATTTTCTGGACATGAAATTTAAACAAGTTCAG
AGAGTACACCAATTAACGAGAGTGGAAATTGAAGCATATTTAAGTGAACTAAACATGATG
GGAATAAAACCTAGTACAATAACTGGGAGGATCTCTATATTGGAAGGACTATTTAGTACC
CTTCTTAGGCTAGAATGGGATGATGTTCCTTCCAAAATATTAATTTATTCTGAGGACTAT
CCGAAAATACCAAGAGCAAAACCACGCTTTATAGATGAATTCGTCCTAGAGCAATTGAAC
AGTCATCTTGATAAATTACCCGAATATATAGCTACGATGACTATGATTGTTCAAGAATGT
GGAATGAGGATAAGTGAATTGTGCACCTTGAAAAAAGGCTGTCTATTAGAGGACAAAGAT
GGAGATTTCTTTTTAAAGTATTATCAATGGAAAATGAAAAAGGAGCATATAGTTCCAATA
TCTAAAGAGGTAGCTTTACTTATTAAAGTTCGGGAAGATAAAGTTTCAGAGGAATTTCCA
GATAGTGAATACCTCTTTCCAAGAAAAGATGGATCGCCATTAAAACAAGAAACATTTAGA
GGTGAGTTAAATAAATTAGCTTATGAGCAAAATATAGTGGATAAATCAGGTGAGATTTAT
AGATTCCATGCCCATGCCTTTCGCCATACAGTAGGAACAAGAATGATTAACAACGGGATG
CCCCAGCATATTGTGCAGAAATTTTTGGGGCATGAAAGCCCAGAAATGACAAGCAGATAC
GCTCATATCTTTGATGAAACTCTAAAAAATGAATTTACTAAATTTCAGGAAAAACTGGTT
ACCAATAATGGAGATGTGCTTGATCTAGATGAAGATAATGAAGTCGATGATGTAGAGCTT
CAATGGTTCAAGAAAAATATAAATGCACAAGTGCTTCCAAATGGTTATTGTAGATTGCCA
GTAGTAGCAGGTGGTTGTCCACATGCGAATGCATGCTTAGATTGCACTCACTTCTGTACC
AGTAAGCAATTCTTACCACAGCACGAAGAACAGTTAGAGCGTACAGAAGAGTTATTAGCC
ATAGCTAAGGATAAACAATGGCAAAGACAAGTAGAGACTAATAGCCGTGTTAAAGAGCGT
TTAGAACAAATCATTGGAAGTTTGACGGGGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1954 [new locus tag: SA_RS11215 ]
- symbol: TnpB
- description: transposition regulatory protein tnpB
- length: 630
- theoretical pI: 8.35267
- theoretical MW: 74438.9
- GRAVY: -0.568889
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 95.1)DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 77.8)and 2 moreDNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 29.9)Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 29.9)
- TheSEED :
- Transposase B from transposon Tn554
- PFAM: DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 151.8)and 4 morel-integrase_N (CL0469) Phage_int_SAM_4; Phage integrase, N-terminal SAM-like domain (PF13495; HMM-score: 27.6)Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 21.6)DNA-mend (CL0382) Phage_integr_3; Archaeal phage integrase (PF16795; HMM-score: 17.5)l-integrase_N (CL0469) Phage_int_SAM_2; Phage integrase, N-terminal (PF12834; HMM-score: 13.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.011768
- TAT(Tat/SPI): 0.001674
- LIPO(Sec/SPII): 0.001624
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)