PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2066 [new locus tag: SA_RS11885 ]
• pan locus tag^?: SAUPAN005732000
• symbol: moaE
• pan gene symbol^?: moaE
• synonym:
• product: molybdopterin converting factor moa (subunit 2)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2066 [new locus tag: SA_RS11885 ]
• symbol: moaE
• product: molybdopterin converting factor moa (subunit 2)
• replicon: chromosome
• strand: -
• coordinates: 2328539..2328985
• length: 447
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124989 NCBI
• RefSeq: NP_375384 NCBI
• BioCyc: see SA_RS11885
• MicrobesOnline: 104410 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  
  ATGAAACAATTTGAAATCGTGATAGAACCGATACAAACAGAACAATATCGTGAATTCACT
  ATAAATGAATATCAAGGTGCAGTAGTTGTTTTTACCGGTCATGTTCGCGAATGGACTAAA
  GGCGTCAAAACGGAATATTTAGAATATGAAGCGTATATTCCAATGGCTGAAAAGAAATTG
  GCACAAATTGGAGATGAAATAAATGAAAAATGGCCTGGAACGATAACGAGTATTGTTCAT
  AGAATAGGGCCATTACAAATTTCAGATATCGCTGTATTAATTGCAGTTTCTTCACCGCAT
  CGTAAAGATGCCTATCGAGCAAATGAATATGCAATTGAGCGTATAAAAGAAATTGTTCCG
  ATTTGGAAAAAAGAAATTTGGGAAGATGGTTCAAAATGGCAAGGGCATCAAAAAGGGAAT
  TATGAAGAAGCGAAGAGGGAGGAATAA
  60
  120
  180
  240
  300
  360
  420
  447
  

⊟Protein

• 2Q5W
  PDB
• 2QIE
  PDB

Protein Data Bank: 2Q5W

Protein Data Bank: 2QIE

⊟General

• locus tag: SA2066 [new locus tag: SA_RS11885 ]
• symbol: MoaE
• description: molybdopterin converting factor moa (subunit 2)
• length: 148
• theoretical pI: 5.35216
• theoretical MW: 17350.6
• GRAVY: -0.629054

⊟Function

• reaction:
  EC 2.8.1.12?  ExPASy
  Molybdopterin synthase Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH₂-C(O)SH + H₂O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
• TIGRFAM:
• TheSEED  :
  • Molybdopterin synthase catalytic subunit MoaE (EC 2.8.1.12)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum cofactor biosynthesis protein MoaE
• PFAM:
  no clan defined MoaE; MoaE protein (PF02391; HMM-score: 119.2)
  and 1 more
  Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 14.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002746
  • TAT(Tat/SPI): 0.000353
  • LIPO(Sec/SPII): 0.000286
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927851 NCBI
• RefSeq: NP_375384 NCBI
• UniProt: P65401 UniProt

⊟Protein sequence

• MKQFEIVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: moaA < mobA < moaD < moaE < mobB < moeA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Juma N Daniels, Margot M Wuebbens, K V Rajagopalan, Hermann Schindelin
Crystal structure of a molybdopterin synthase-precursor Z complex: insight into its sulfur transfer mechanism and its role in molybdenum cofactor deficiency.
Biochemistry: 2008, 47(2);615-26
[PubMed:18092812] [WorldCat.org] [DOI] (P p)