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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2098 [new locus tag: SA_RS12060 ]
  • pan locus tag?: SAUPAN005775000
  • symbol: SA2098
  • pan gene symbol?:
  • synonym:
  • product: glycerate dehydrogenase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2098 [new locus tag: SA_RS12060 ]
  • symbol: SA2098
  • product: glycerate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2358178..2359131
  • length: 954
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGGTTAAAACTTTTACAAGAA
    CATTTTGAAGTTGAAATGTATGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAA
    GGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTACAAACGTTGATAAAGATGTT
    ATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
    GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACA
    AACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCTCGTCGTATTGTTGAA
    GGGGACCAATTATCACGTACAACTGGATTTGACGGATGGGCACCTTTATTTTTCAGAGGT
    AGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
    GCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATCGCAAAGAA
    GAAAAAGAACGAGAAATCGGTGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCA
    GATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGCATCATTTAATTGATACAGAA
    CAATTTAAAATGATGAAATCTACGGTGTATTTAATCAATGCCTCTCGTGGTCCAATCGTG
    CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGAT
    GTATATGAATTTGAACCAGATATTACCGATGACTTAAAATCACTTAATAATGTAGTATTA
    ACACCGCATATTGGTAATGCTACATTTGAAGCACGTGACATGATGTCTAAAATTGTTGCT
    AATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2098 [new locus tag: SA_RS12060 ]
  • symbol: SA2098
  • description: glycerate dehydrogenase
  • length: 317
  • theoretical pI: 4.91492
  • theoretical MW: 34702.4
  • GRAVY: -0.119874

Function[edit | edit source]

  • reaction:
    EC 1.1.1.-?  ExPASy
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 269.3)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 15.2)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 11.9)
  • TheSEED  :
    • Glyoxylate reductase (EC 1.1.1.26)
    • Glyoxylate reductase (NADP+) (EC 1.1.1.79)
    • Hydroxypyruvate reductase (EC 1.1.1.81)
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.26)
    and 3 more
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.79)
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Hydroxypyruvate reductase (EC 1.1.1.81)
    Carbohydrates Organic acids Glycerate metabolism  Hydroxypyruvate reductase (EC 1.1.1.81)
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 201.9)
    and 5 more
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 113.3)
    NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 20)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 19.4)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16.8)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 12.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001313
    • TAT(Tat/SPI): 0.000138
    • LIPO(Sec/SPII): 0.000235
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

  • SA2098 no polycistronic organisation predicted

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)