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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2142 [new locus tag: SA_RS12300 ]
  • pan locus tag?: SAUPAN005852000
  • symbol: SA2142
  • pan gene symbol?: emrB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2142 [new locus tag: SA_RS12300 ]
  • symbol: SA2142
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2406617..2408548
  • length: 1932
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACTACGACCTTCATTATTAGCTACATTATTTTAGCGCTCATTATTGTTGGGGTTATC
    AATTTATTTTTAATAAGATCAAGAAAAAAAGGCAAACGCCAACAAAAGGAACAACAATTT
    ACGACACGTCAATCAAATCAATCTAAATTTAAAGCTAGTGATTTAGACAAAACAACTGAT
    CAATCGACACAACGTATGACGCATGAAGAGTTGCGTGTTGACAATCAAGATGATCATAGC
    CAAGTTAGTCTAAATGGTTACACAAAGGGGTCTGAGAAAGATCAAGAAGCATTCACTAAT
    AATAATGGTGAGGAAGCAGTTGCTGCTAAAAATCCTGAATCAGAAGAATATAAAGTGAAT
    GAAAAAATAAAAAAAGAACATAAAAACTTTATTTTTGGTGAAGGTGTTTCACGCGGTAAA
    ATATTAGCGGCATTATTATTCGGTATGTTTATTGCGATTTTAAACCAAACATTATTAAAT
    GTTGCTTTGCCAAAAATTAATACGGAATTTAATATTTCTGCGTCAACAGGGCAATGGTTG
    ATGACGGGATTCATGTTAGTAAACGGTATTTTAATACCAATTACGGCGTATCTATTTAAT
    AAATATTCATATCGAAAATTATTTTTAGTTGCGTTAGTACTCTTTACAATTGGTTCATTA
    ATCTGTGCGATTTCAATGAATTTCCCAATCATGATGGTTGGACGTGTATTACAAGCCATT
    GGTGCAGGTGTACTAATGCCATTAGGTTCAATTGTTATTATTACAATTTATCCACCTGAA
    AAACGTGGTGCTGCCATGGGTACAATGGGTATCGCAATGATTTTAGCACCTGCAATTGGT
    CCAACATTATCAGGTTATATTGTACAAAATTACCATTGGAATGTGATGTTTTACGGTATG
    TTCATTATCGGTATTATTGCAATCTTAGTTGGTTTTGTATGGTTTAAGTTATATCAATAT
    ACAACAAACCCTAAAGCTGATATCCCAGGTATCATTTTTAGTACGATTGGTTTTGGTGCT
    TTGTTATACGGCTTTTCAGAAGCGGGGAACAAAGGATGGGGTTCTGTTGAGATAGAAACA
    ATGTTTGCGATTGGTATTATCTTTATTATTCTATTTGTAATAAGAGAATTAAGAATGAAA
    TCACCAATGTTGAATTTAGAAGTATTAAAATTCCCAACATTTACATTAACAACAATTATT
    AATATGGTTGTAATGTTAAGTTTATATGGTGGTATGATTTTATTACCGATTTATTTACAA
    AATTTACGCGGATTCTCAGCATTAGATTCCGGATTGTTATTATTACCTGGTTCTCTAATT
    ATGGGTCTACTTGGACCATTTGCAGGTAAATTGTTAGATACTATTGGTTTAAAACCATTA
    GCTATCTTTGGTATTGCTGTCATGACTTATGCAACATGGGAATTAACTAAATTAAATATG
    GATACACCATATATGACAATCATGGGTATCTATGTACTTCGTTCATTTGGTATGGCATTT
    ATAATGATGCCAATGGTAACTGCAGCTATTAATGCGTTACCGGGACGACTTGCCTCTCAT
    GGTAATGCTTTCTTAAATACGATGCGTCAATTAGCAGGCTCTATAGGTACAGCAATCTTA
    GTTACTGTTATGACAACACAAACAACACAACATTTATCAGCTTTTGGGGAAGAGTTAGAT
    AAAACGAATCCTGTTGTACAAGATCATATGCGTGAATTAGCATCGCAATATGGTGGTCAA
    GAAGGCGCAATGAAAGTGTTATTACAATTTGTAAATAAACTAGCAACAGTTGAAGGTATT
    AATGATGCATTTATAGTTGCAACGATATTTAGCATCATCGCTTTAATTTTATGTTTATTT
    TTACAAAGTAATAAAAAAGCAAAAGCTACAGCTCAAAAGTTAGAAGCAAGCTCAAATAAC
    AATCATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1932

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2142 [new locus tag: SA_RS12300 ]
  • symbol: SA2142
  • description: hypothetical protein
  • length: 643
  • theoretical pI: 9.64323
  • theoretical MW: 70878.3
  • GRAVY: 0.394868

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.6)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 443.6)
    and 10 more
    multidrug resistance protein (TIGR00880; HMM-score: 77.7)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 67.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 66.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 64.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 48.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 24.7)
    oxalate/formate antiporter (TIGR04259; HMM-score: 22.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 14)
    Cellular processes Cellular processes Adaptations to atypical conditions phage shock protein B (TIGR02976; HMM-score: 10.5)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 6.8)
  • TheSEED  :
    • Uncharacterized MFS-type transporter YhcA, EmrB family
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Multidrug Resistance, 2-protein version Found in Gram-positive bacteria  Membrane component of multidrug resistance system
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 191.9)
    and 12 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 35.2)
    no clan defined EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 19.7)
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 14.4)
    Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 14.1)
    ATP_synthase (CL0255) YMF19; Plant ATP synthase F0 (PF02326; HMM-score: 13.4)
    no clan defined DUF1180; Protein of unknown function (DUF1180) (PF06679; HMM-score: 12.6)
    DUF2615; Protein of unknown function (DUF2615) (PF11027; HMM-score: 12.3)
    Shisa; Wnt and FGF inhibitory regulator (PF13908; HMM-score: 12.3)
    DUF2561; Protein of unknown function (DUF2561) (PF10812; HMM-score: 12.2)
    ASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12)
    CcmD; Heme exporter protein D (CcmD) (PF04995; HMM-score: 9.1)
    MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 7.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 15
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.083803
    • TAT(Tat/SPI): 0.001799
    • LIPO(Sec/SPII): 0.024049
  • predicted transmembrane helices (TMHMM): 15

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQSNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNNGEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVVQDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKKAKATAQKLEASSNNNHE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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