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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0050
  • pan locus tag?: SAUPAN000666000
  • symbol: pls
  • pan gene symbol?: pls
  • synonym:
  • product: methicillin-resistant surface protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0050
  • symbol: pls
  • product: methicillin-resistant surface protein
  • replicon: chromosome
  • strand: +
  • coordinates: 57212..61858
  • length: 4647
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAATAAAAATTCGAAGAAGAAGCTCGATTTTCTTCCAAACAAGCTTAATAAGTACTCA
    ATTAGACGTTTCACTGTAGGGACAGCTTCGATTTTAGTAGGAGCTACTTTAATTTTCGGT
    GTTGCAAATGATCAAGCAGAAGCCGCTGAGAATAACACAACTCAAAAGCAAGATGATAGT
    TCAGATGCAAGTAAAGTAAAAGGTAATGTTCAAACTATTGAACAATCTTCTGCAAATTCA
    AATGAATCTGATATTCCTGAACAAGTTGATGTAACTAAAGATACAACTGAACAAGCATCA
    ACAGAAGAAAAAGCAAATACAACTGAACAAGCATCAACAGAAGAAAAAGCAGATACAACT
    GAACAAGCAACAACAGAAGAAGCGCCAAAAGCTGAAGGAACAGACAAAGTAGAAACAGAA
    GAAGCGCCAAAAGCTGAAGAAACAGACAAAGCAACAGAAGAAGCACCAAAAACTGAAGAA
    ACAGACAAAGCAACAACAGAAGAAGCGCCAAAAGCTGAAGAAACAGACAAAGCAACAGAA
    GAAGCACCAAAAACTGAAGAAACAGACAAAGCAACAACAGAAGAAGCGCCAGCAGCTGAA
    GAAACAAGCAAAGCAGCAACAGAAGAAGCGCCAAAAGCTGAAGAAACAAGCAAAGCAGCA
    ACAGAAGAAGCGCCAAAAGCTGAAGAAACAGAAAAAACAGCAACAGAAGAAGCACCAAAA
    ACTGAAGAAACAGACAAAGTAGAAACAGAAGAAGCGCCAAAAGCTGAAGAAACAAGCAAA
    GCAGCAACAGAAAAAGCACCAAAAGCTGAAGAAACAAACAAAGTAGAAACAGAAGAAGCG
    CCAGCAGCTGAAGAAACAAACAAAGCAGCAACAGAAGAAACACCAGCAGTTGAAGACACA
    AATGCTAAGAGCAATTCAAATGCTCAACCATCAGAAACTGAGAGAACTCAAGTTGTAGAT
    ACAGTTGCTAAAGATTTATATAAAAAATCTGAAGTTACAGAAGCAGAAAAAGCTGAAATT
    GAAAAAGTATTACCAAAAGATATTTCAAACTTATCTAATGAAGAAATTAAAAAAATAGCT
    TTAAGTGAAGTACTTAAAGAAACAGCTAACAAAGAAAACGCACAACCAAGAGCAACATTC
    CGTTCAGTAAGCAGCAATGCTAGAACAACAAATGTTAACTATTCAGCAACAGCATTAAGA
    GCAGCTGCACAAGACACAGTTACTAAAAAAGGAACTGGTAACTTTACTGCGCATGGAGAT
    ATAATCCATAAAACTTATAAAGAAGAATTCCCTAATGAAGGCACGCTAACTGCATTCAAT
    ACAAACTTCAATCCTAATACAGGAACTAAAGGCGCATTAGAATATAATGATAAAATAGAT
    TTTAATAAAGACTTTACAATTACTGTTCCAGTAGCAAACAACAACCAAGGTAATACAACA
    GGAGCAGATGGCTGGGGCTTCATGTTTACTCAAGGGAATGGCCAAGACTTCTTAAACCAA
    GGTGGTATTTTAAGAGACAAAGGTATGGCAAATGCATCTGGTTTTAAAATTGATACGGCA
    TATAATAATGTTAATGGTAAAGTCGATAAACTCGATGCAGATAAAACAAACAATCTAAGT
    CAAATTGGCGCAGCAAAAGTTGGTTACGGTACATTTGTTAAAAATGGTGCAGATGGTGTG
    ACTAACCAAGTTGGTCAAAATGCCCTAAATACAAAAGATAAACCTGTAAATAAAATAATT
    TATGCAGATAATACAACTAATCATCTTGATGGTCAATTCCATGGCCAAAGATTAAATGAT
    GTAGTATTAAATTATGATGCAGCAACAAGTACAATAACTGCTACATATGCAGGAAAAACA
    TGGAAAGCTACTACAGATGATTTAGGAATTGATAAATCACAAAAATATAATTTCTTAATT
    ACTTCAAGTCATATGCAAAATAGATATTCTAATGGAATTATGAGAACAAATCTTGAAGGT
    GTAACAATTACAACGCCTCAAGCTGATTTAATTGATGATGTGGAAGTAACGAAACAACCA
    ATTCCTCATAAAACTATTCGTGAGTTTGATCCAACTCTAGAACCAGGCTCACCTGATGTT
    ATTGTACAAAAAGGTGAAGATGGAGAGAAAACAACAACTACACCAACTAAAGTTGACCCT
    GATACAGGAGATGTAGTTGAACGTGGTGAACCAACAACAGAAGTTACAAAAAATCCAGTT
    GACGAGATTGTACACTTTGCACCTGAAGAAGTACCACAAGGTCATAAAGATGAGTTCGAT
    CCAAACTTACCAATTGACGGTACAGAAGAAGTACCAGGTAAACCAGGCATCAAGAATCCT
    GAAACAGGTGAAGTAGTAACACCTCCGGTTGACGATGTCACAAAACATGGTCCAAAAGCA
    GGCGAACCAGAGGTTACTAAAGAAGAAATACCATTCGAGAAAAAACGTGAGTTCAATCCA
    GACTTAAAACCAGGTGAAGAGAAAGTAACGCAAGAAGGACAAACTGGAGAGAAAACAACA
    ACAACGCCAACAACAATTAATCCATTAACGGGAGAAAAAGTAGGCGAAGGTGAACCAACA
    ACAGAAGTAACAAAAGAACCAGTAGATGAAATCACACAATTCGGTGGAGAAGAAGTACCA
    CAAGGTCATAAAGATGAGTTCGATCCAAACTTACCAATTGACGGTACAGAAGAAGTACCA
    GGTAAACCAGGCATCAAGAATCCTGAAACAGGTGAAGTAGTAACACCTCCGGTTGACGAT
    GTCACAAAACATGGTCCAAAAGCAGGCGAACCAGAGGTTACTAAAGAAGAAATACCATTC
    GAGAAAAAACGTGAGTTCAATCCAGACTTAAAACCAGGTGAAGAGAAAGTAACGCAAGAA
    GGACAAACTGGAGAGAAAACAACAACAACGCCAACAACAATTAATCCATTAACGGGAGAA
    AAAGTAGGCGAAGGTGAACCAACAACAGAAGTAACAAAAGAACCAGTAGATGAAATCACA
    CAATTCGGTGGAGAAGAAGTACCACAAGGTCATAAAGATGAGTTCGATCCAAACTTACCA
    ATTGACGGTACAGAAGAAGTACCAGGTAAACCAGGCATCAAGAATCCTGAAACAGGTGAA
    GTAGTAACACCACCAGTAGACGATGTCACAAAACATGGTCCAAAAGCAGGCGAACCAGAG
    GTTACTAAAGAAGAAATTCCATATGAAACTAAACGCGTATTAGATCCAACAATGGAACCA
    GGTAGTCCTGATAAAGTAGCTCAAAAAGGTGAAAATGGTGAAAAAACAACAACAACACCA
    ACTACAATTAATCCATTAACGGGAGAAAAAGTAGGCGAAGGCGAACCAACAACGGAAGTA
    ACGAAAGAACCAATAGACGAAATTGTTAACTATGCACCTGAAATTATTCCTCATGGTACA
    CGTGAAGAAATTGATCCAAACTTACCAGAAGGTGAAACTAAAGTTATCCCAGGTAAAGAT
    GGCTTGAAAGATCCTGAAACTGGAGAAATCATTGAAGAACCACAAGATGAAGTAATCATC
    CATGGTGCTAAAGATGATTCAGATGCGGACAGCGATTCAGACGCAGATAGCGATTCTGAT
    GCAGACAGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGATTCAGACAGCGAT
    AGCGATTCAGATTCAGATAGCGACTCTGATGCGGACAGCGATTCAGACGCAGATAGCGAT
    TCAGATTCAGATAGTGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGACTCTGAT
    GCGGACAGCGACTCAGACGCAGATAGCGATTCTGATTCAGACAGCGACTCAGACGCAGAT
    AGCGACTCAGATTCAGACAGCGATTCAGACGCAGATAGCGACTCAGACGCAGATAGCGAT
    TCAGACGCAGATAGCGACTCAGACGCAGATAGCGATTCAGATTCAGATAGCGACTCTGAT
    GCGGACAGCGATAGCGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGATTCAGAC
    AGCGATTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAC
    TCAGACGCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATAGCGAT
    TCAGATTCAGACAGAGACTCAGACGCAGATAGCGACTCAGACGCAGATAGCGACTCAGAT
    TCAGACAGCGATTCAGACGCAGATAGCGATTCAGATTCAGATAGTGACTCTGATGCGGAC
    AGCGATTCAGACGCAGATAGCGACTCAGATTCAGACGCAGATAGCGATTCTGATTCAGAC
    AGCGACTCAGACGCAGATAGCGACTCTGATGCGGACAGCGACTCAGACGCAGATAGCGAT
    TCTGATGCAGACAGCGACTCAGACAGCGATAGCGATTCTGATTCAGACAGCGATTCAGAC
    GCAGATAGCGACTCTGATGCGGACAGCGATTCAGACGCAGATAGAGATCATAATGACAAA
    ACAGATAAACCAAATAATAAAGAGTTACCAGATACTGGTAATGATGCTCAAAATAATGGC
    ACATTATTTGGTTCACTATTCGCTGCGCTTGGAGGATTATTCTTAGTTGGCAGACGTCGT
    AAAAACAAAAATAATGAAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4320
    4380
    4440
    4500
    4560
    4620
    4647

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0050
  • symbol: Pls
  • description: methicillin-resistant surface protein
  • length: 1548
  • theoretical pI: 3.78806
  • theoretical MW: 164847
  • GRAVY: -1.19541

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 50.1)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.6)
  • TheSEED  :
    • Antiadhesin Pls, binding to squamous nasal epithelial cells
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Antiadhesin Pls, binding to squamous nasal epithelial cells
    and 1 more
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Antiadhesin Pls, binding to squamous nasal epithelial cells
  • PFAM:
    G5 (CL0593) SasG_E; E domain (PF17041; HMM-score: 294.7)
    and 4 more
    G5; G5 domain (PF07501; HMM-score: 122.5)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 42.1)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 41.8)
    2C_adapt; 2-cysteine adaptor domain (PF08793; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: found LPxTG motif :LPDTG
  • SignalP: Signal peptide SP(Sec/SPI) length 48 aa
    • SP(Sec/SPI): 0.860587
    • TAT(Tat/SPI): 0.113146
    • LIPO(Sec/SPII): 0.00261
    • Cleavage Site: CS pos: 48-49. AEA-AE. Pr: 0.9469
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNSKKKLDFLPNKLNKYSIRRFTVGTASILVGATLIFGVANDQAEAAENNTTQKQDDSSDASKVKGNVQTIEQSSANSNESDIPEQVDVTKDTTEQASTEEKANTTEQASTEEKADTTEQATTEEAPKAEGTDKVETEEAPKAEETDKATEEAPKTEETDKATTEEAPKAEETDKATEEAPKTEETDKATTEEAPAAEETSKAATEEAPKAEETSKAATEEAPKAEETEKTATEEAPKTEETDKVETEEAPKAEETSKAATEKAPKAEETNKVETEEAPAAEETNKAATEETPAVEDTNAKSNSNAQPSETERTQVVDTVAKDLYKKSEVTEAEKAEIEKVLPKDISNLSNEEIKKIALSEVLKETANKENAQPRATFRSVSSNARTTNVNYSATALRAAAQDTVTKKGTGNFTAHGDIIHKTYKEEFPNEGTLTAFNTNFNPNTGTKGALEYNDKIDFNKDFTITVPVANNNQGNTTGADGWGFMFTQGNGQDFLNQGGILRDKGMANASGFKIDTAYNNVNGKVDKLDADKTNNLSQIGAAKVGYGTFVKNGADGVTNQVGQNALNTKDKPVNKIIYADNTTNHLDGQFHGQRLNDVVLNYDAATSTITATYAGKTWKATTDDLGIDKSQKYNFLITSSHMQNRYSNGIMRTNLEGVTITTPQADLIDDVEVTKQPIPHKTIREFDPTLEPGSPDVIVQKGEDGEKTTTTPTKVDPDTGDVVERGEPTTEVTKNPVDEIVHFAPEEVPQGHKDEFDPNLPIDGTEEVPGKPGIKNPETGEVVTPPVDDVTKHGPKAGEPEVTKEEIPFEKKREFNPDLKPGEEKVTQEGQTGEKTTTTPTTINPLTGEKVGEGEPTTEVTKEPVDEITQFGGEEVPQGHKDEFDPNLPIDGTEEVPGKPGIKNPETGEVVTPPVDDVTKHGPKAGEPEVTKEEIPFEKKREFNPDLKPGEEKVTQEGQTGEKTTTTPTTINPLTGEKVGEGEPTTEVTKEPVDEITQFGGEEVPQGHKDEFDPNLPIDGTEEVPGKPGIKNPETGEVVTPPVDDVTKHGPKAGEPEVTKEEIPYETKRVLDPTMEPGSPDKVAQKGENGEKTTTTPTTINPLTGEKVGEGEPTTEVTKEPIDEIVNYAPEIIPHGTREEIDPNLPEGETKVIPGKDGLKDPETGEIIEEPQDEVIIHGAKDDSDADSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDSDSDSDADSDSDADSDSDSDSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDSDSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDSDSDSDSDADSDSDSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDSDSDSDSDSDRDSDADSDSDADSDSDSDSDSDADSDSDSDSDSDADSDSDADSDSDSDADSDSDSDSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDSDSDSDADSDSDADSDSDADRDHNDKTDKPNNKELPDTGNDAQNNGTLFGSLFAALGGLFLVGRRRKNKNNEEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Sortase substrate [1] [2] [3] [4] [5]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 8.74 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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