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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0052 [new locus tag: SACOL_RS00275 ]
  • pan locus tag?: SAUPAN000668000
  • symbol: SACOL0052
  • pan gene symbol?: —
  • synonym:
  • product: glycosyl transferase, group 1 family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0052 [new locus tag: SACOL_RS00275 ]
  • symbol: SACOL0052
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 63758..65269
  • length: 1512
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATATATTCAGTAGGGAATAGGTTAGGAAATAAATTAACCGGAATTGAAAAAGCGATG
    ATTAATAGACACAACTTATTTAAAAATCACTCGATAACTAGTAAATTGATTTTTGCATCG
    TGGTCACCTCGTTTACATTTTAATGCAAGTCTATTTCAAATTGAAGATGACGATATATTA
    AGCTTATATGATGTGCTACAAGACAGTATAAATATAAAAAGCATCCATAAGGACTGGATC
    ACTCATTGGACTAAAGAGTGTAATTATACACTAAAATTTATTGAAAATACCAATGATATA
    AAGATATATGAAAAAAGCAACTATAGAATGTATGTCCATTTTAATGATAGTAATTACCAA
    CATTTGGATTACATAAATCATTTTGATGTAAATCAACGTAAAATACGTCGAGATCTATAT
    GATTCAAGAGGCTTTTTAAGTTGTTCAAGAACTTTAACAACAAATCAAAAAGTAGTTTGT
    GAACATTATTACACTCCTGAAGGTACTATAAAGTTCCAAAAATTTTTTAATCCAGAACTT
    GAAAATTCACAATCACAACTTATTATTTATAACACCGATTCTCAACTTAAGTATTTTAAT
    AATGATCAGGAATTACTAGCATTTGCAATTGAAAAATTGTATAAAAATGGAGATATTTTC
    CTGAGTGATAAAAATATAAATACTGCACCAATATTCAATAATACTAGTGAAACAATACCT
    GTTTTAGCTGTTTTGCATAGTACTCATGTCAAGAATATTGACATGGTATATGAATCGGAC
    ATCAAAAATACTTATAAACATGTTTTTAACAATCTAAATAGATACTCTGGGATAATTGTA
    TCGACTAAACAGCAACAATTGGATATATCAGCTCGAATAAATAATGAAATCCCTGTTCAT
    ACTATACCAGTAGGATATATTGATGAACATTTTACTAATTTAAAAAGAAATAATCATAGT
    ATAAATAATAATAAAATTATTTCCGTTGCACGTTATTCTCCTGAAAAACAGTTGAATCAT
    CAAATTGAATTAGTTTCTAAATTAATTAAAGAATTTCCTAACATCCAACTACATTTGTAT
    GGGTTTGGTAAAGAAGAAGAAAAATACAAGCAACTAATCACTGAATATAATTTAGAAAAC
    AATGTGTTTTTACGAGGGTTTAGAAGAAATTTAAGTGCTGAGATTCAAGATGCTTATATG
    TCTCTAATTACTAGTAATATGGAAGGATTTAATTTAGGTTTATTAGAAACTATAACTGAA
    GGCATACCTCCAGTTGGCTATAATTCGAAGTATGGTCCTTCCGAATTAATATTAAATAAT
    GAAAATGGTTATTTAATTAACAAAAATGATAAAGATGAATTATATAATCGAGTGCGAAAC
    CTATTATTAGATAAGACTTTAAGAGATACTTTTTCTCAAGAATGTATCAAGCACTCTAAA
    GCTTTTTCAAGTAAAATTGTTATGAAGTTATGGGAAGATAGATTTTCTACAATTAATTCA
    AGTTATGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1512

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0052 [new locus tag: SACOL_RS00275 ]
  • symbol: SACOL0052
  • description: glycosyl transferase, group 1 family protein
  • length: 503
  • theoretical pI: 7.52856
  • theoretical MW: 59042.3
  • GRAVY: -0.556461

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 132.3)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 76.2)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 47.4)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 42.4)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 24.6)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 22.4)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 16.2)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 13.1)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 10.4)
  • TheSEED  :
    • Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 97.2)
    and 2 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 56.9)
    no clan defined YccV-like; Hemimethylated DNA-binding protein YccV like (PF08755; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004021
    • TAT(Tat/SPI): 0.000451
    • LIPO(Sec/SPII): 0.000509
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIYSVGNRLGNKLTGIEKAMINRHNLFKNHSITSKLIFASWSPRLHFNASLFQIEDDDILSLYDVLQDSINIKSIHKDWITHWTKECNYTLKFIENTNDIKIYEKSNYRMYVHFNDSNYQHLDYINHFDVNQRKIRRDLYDSRGFLSCSRTLTTNQKVVCEHYYTPEGTIKFQKFFNPELENSQSQLIIYNTDSQLKYFNNDQELLAFAIEKLYKNGDIFLSDKNINTAPIFNNTSETIPVLAVLHSTHVKNIDMVYESDIKNTYKHVFNNLNRYSGIIVSTKQQQLDISARINNEIPVHTIPVGYIDEHFTNLKRNNHSINNNKIISVARYSPEKQLNHQIELVSKLIKEFPNIQLHLYGFGKEEEKYKQLITEYNLENNVFLRGFRRNLSAEIQDAYMSLITSNMEGFNLGLLETITEGIPPVGYNSKYGPSELILNNENGYLINKNDKDELYNRVRNLLLDKTLRDTFSQECIKHSKAFSSKIVMKLWEDRFSTINSSYE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 110 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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