PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0081
• pan locus tag^?: SAUPAN000864000
• symbol: SACOL0081
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0081
• symbol: SACOL0081
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 91050..91214
• length: 165
• essential: unknown

⊟Accession numbers

• Gene ID: 3236871 NCBI
• RefSeq: YP_184986 NCBI
• BioCyc:
• MicrobesOnline: 911561 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  
  ATGATGAAACGATTAAACAAATTAGTGTTAGGCATTATTTTTCTGTTTTTAGTCATTAGT
  ATCACTGCTGGTTGTGGCATAGGTAAAGAAGCGAAAATAAAGAAAAGTTTTGAGAAGACA
  TTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGAATAA
  60
  120
  165
  

⊟Protein

⊟General

• locus tag: SACOL0081
• symbol: SACOL0081
• description: hypothetical protein
• length: 54
• theoretical pI: 10.0244
• theoretical MW: 6150.5
• GRAVY: 0.383333

⊟Function

• TIGRFAM:
  Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 68.7)
  and 1 more
  Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 14.6)
• TheSEED  :
  • FIG01107885: hypothetical protein
• PFAM:
  LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 64.4)
  and 2 more
  4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 12.6)
  no clan defined Spore_III_AF; Stage III sporulation protein AF (Spore_III_AF) (PF09581; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: ITAGCGI
• SignalP: Signal peptide LIPO(Sec/SPII) length 24 aa
  • SP(Sec/SPI): 0.001544
  • TAT(Tat/SPI): 0.000105
  • LIPO(Sec/SPII): 0.998021
  • Cleavage Site: CS pos: 24-25. TAG-CG. Pr: 0.9986
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 57652445 NCBI
• RefSeq: YP_184986 NCBI
• UniProt: Q5HJS0 UniProt

⊟Protein sequence

• MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMYPIKNLEDLYE

⊟Experimental data

• experimentally validated: PeptideAtlas
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications