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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • pan locus tag?: SAUPAN000957000
  • symbol: SACOL0128
  • pan gene symbol?: phnD
  • synonym:
  • product: phosphonate ABC transporter phosphonate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • symbol: SACOL0128
  • product: phosphonate ABC transporter phosphonate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 143021..143977
  • length: 957
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATTTTAAGTGTTTATTTGTATTAATGTTAGCAGTCATTGTTTTTGCAGCAGCA
    TGTGGAAACTCAAGTTCTTTAGATAATCAAAAGAACGCTAGTAATGATTCGGATTCTAAA
    TCAGGAGGATACAAACCTAAAGAATTAACCGTTCAATTTGTACCTTCGCAAAATGCTGGA
    ACATTAGAAGCTAAAGCAAAACCATTAGAAAAATTACTATCTAAAGAATTAGGGATTCCA
    GTTAAAGTGTCTGTATCAACTAACTACAATACAATTGTTGAAGCTATGAAGTCTAAAAAA
    GTTGATGTTGGTTTCTTACCACCAACGGCATACACATTAGCACATGATCAAAAAGCAGCT
    GATTTATTATTACAAGCACAACGTTTCGGTGTAAAAGAAGATGGTTCAGCAAGTAAAGAA
    CTTGTAGATAGTTATAAATCAGAAATTCTTGTTAAAAAAGACTCAAAAATTAAAAGCTTG
    AAAGATTTAAAAGGTAAGAAAATTGCCTTACAAGATGTAACATCAACTGCTGGATATACA
    TTCCCACTTGCGATGTTAAAAAACGAAGCAGGTATTAATGCAACTAAAGATATGAAAATT
    GTGAATGTTAAAGGTCATGACCAAGCAGTTATCTCATTATTAAATGGAGATGTAGATGCT
    GCGGCTGTATTTAACGATGCACGTAATACTGTGAAAAAAGACCAACCAAATGTATTTAAA
    GACACACGAATTTTAAAATTAACACAAGCTATTCCGAATGACACAATTTCTGTAAGACCA
    GATATGGATAAAGATTTTCAAGAAAAATTGAAAAAAGCTTTTATAGACATTGCTAAATCA
    AAAGAAGGTCACAAAATTATTAGCGAAGTTTATTCACATGAAGGATACACAGAAACGAAA
    GATTCAAATTTCGACATTGTAAGAGAGTACGAAAAATTAGTTAAAGATATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    957

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0128 [new locus tag: SACOL_RS00645 ]
  • symbol: SACOL0128
  • description: phosphonate ABC transporter phosphonate-binding protein
  • length: 318
  • theoretical pI: 9.66321
  • theoretical MW: 35000
  • GRAVY: -0.393082

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein (TIGR01098; HMM-score: 257.2)
    and 6 more
    phosphonate ABC transporter, periplasmic phosphonate binding protein (TIGR03431; HMM-score: 173.6)
    putative selenate ABC transporter periplasmic binding protein (TIGR04553; HMM-score: 76.2)
    Metabolism Transport and binding proteins Other ABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 54.3)
    taurine ABC transporter, periplasmic binding protein (TIGR01729; HMM-score: 46.2)
    Metabolism Transport and binding proteins Unknown substrate TRAP transporter solute receptor, TAXI family (TIGR02122; HMM-score: 37.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 22.2)
  • TheSEED  :
    • Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1)
    Membrane Transport ABC transporters ABC transporter alkylphosphonate (TC 3.A.1.9.1)  Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1)
  • PFAM:
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 241.7)
    and 6 more
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 48.4)
    SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 44.1)
    NMT1_2; NMT1-like family (PF13379; HMM-score: 24.1)
    Mycoplasma_p37; High affinity transport system protein p37 (PF06646; HMM-score: 23.8)
    NMT1_3; NMT1-like family (PF16868; HMM-score: 21.3)
    Hexosaminidase (CL0546) Glyco_hydro_20b; Glycosyl hydrolase family 20, domain 2 (PF02838; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: VFAAACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.001797
    • TAT(Tat/SPI): 0.000106
    • LIPO(Sec/SPII): 0.997879
    • Cleavage Site: CS pos: 20-21. AAA-CG. Pr: 0.9982
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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