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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0263 [new locus tag: SACOL_RS01335 ]
  • pan locus tag?: SAUPAN001163000
  • symbol: lytM
  • pan gene symbol?: lytM
  • synonym:
  • product: peptidoglycan hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0263 [new locus tag: SACOL_RS01335 ]
  • symbol: lytM
  • product: peptidoglycan hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 301648..302616
  • length: 969
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAGGATGTTTTATACATGAAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTC
    GCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCA
    CATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGAT
    TCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAAT
    AAAGAATATAGCTATACATTCGTAGATGCTCAAGGACATACGCATTATTTTTATAACTGT
    TATCCAAAAAATGCAAATGCCAATGGAAGCGGCCAAACATATGTGAATCCAGCAACAGCA
    GGAGATAACAATGACTACACAGCGAGTCAAAGCCAACAGCATATTAATCAATATGGTTAT
    CAATCAAATGTAGGTCCAGACGCGAGCTATTATTCACATAGTAACAACAACCAAGCGTAT
    AACAGCCATGATGGTAATGGAAAGGTCAATTATCCTAATGGCACATCTAATCAAAATGGT
    GGATCAGCAAGTAAAGCGACAGCTAGTGGTCATGCGAAAGACGCAAGCTGGTTAACAAGT
    CGTAAACAACTACAACCATATGGACAATATCACGGTGGTGGTGCGCATTACGGTGTCGAC
    TATGCAATGCCTGAAAATTCACCAGTTTACTCATTAACTGATGGTACAGTAGTACAAGCA
    GGTTGGAGTAACTATGGTGGCGGCAATCAAGTAACGATTAAAGAAGCGAACAGTAATAAC
    TACCAATGGTATATGCATAATAATCGTTTAACTGTTTCAGCTGGTGATAAAGTCAAAGCT
    GGTGACCAAATTGCATATTCAGGTAGTACGGGTAATTCAACAGCGCCTCACGTACACTTC
    CAACGTATGTCTGGTGGCATCGGTAATCAATATGCAGTAGACCCAACGTCATACTTGCAA
    AGTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0263 [new locus tag: SACOL_RS01335 ]
  • symbol: LytM
  • description: peptidoglycan hydrolase
  • length: 322
  • theoretical pI: 6.44802
  • theoretical MW: 35067.2
  • GRAVY: -0.92205

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.75?  ExPASy
    Lysostaphin Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans
  • TIGRFAM:
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta' subunit (TIGR02386; EC 2.7.7.6; HMM-score: 11.2)
  • TheSEED  :
    • Glycyl-glycine endopeptidase LytM precursor (EC 3.4.24.75)
    Protein Metabolism Protein degradation EC 3.4.24.- Metalloendopeptidases  Glycyl-glycine endopeptidase LytM precursor (EC 3.4.24.75)
  • PFAM:
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 94.2)
    and 1 more
    no clan defined DUF4124; Domain of unknown function (DUF4124) (PF13511; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: HQADAAET
  • SignalP: Signal peptide SP(Sec/SPI) length 31 aa
    • SP(Sec/SPI): 0.943209
    • TAT(Tat/SPI): 0.055291
    • LIPO(Sec/SPII): 0.000661
    • Cleavage Site: CS pos: 31-32. ADA-AE. Pr: 0.9604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEDVLYMKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQDVSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANANGSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGNGKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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