From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0295 [new locus tag: SACOL_RS01495 ]
  • pan locus tag?: SAUPAN001209000
  • symbol: SACOL0295
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0295 [new locus tag: SACOL_RS01495 ]
  • symbol: SACOL0295
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 331507..332007
  • length: 501
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGACTTTCGAAGAGAAAATAAGCAAATTATATAATGAGATTGCGAATGAGATTAGCAGT
    ATGATACCGGTAGAGTGGGAAAAAGTATATACAATGGCTTATATAGATGATGGAGGAGGT
    GAAGTATTCTTTAATTATACTAAACCAGGAAGTGAAGATTTGAATTATTATACCGATATA
    CCTAAGGAGTATAATGTTTCTGTGCAAGTATTTGATGATTTATGGATGGATTTATATGAT
    TTGTTTAAGAATTTAAGAAATTTATTTAAAGAAGAAGGACTTGAACCATGGACATCATGT
    GAATTTGACTTTACAAGAGACGGCAAATTGAATGTTTCATTTGATTATATTGATTGGGCG
    AATTCAGAGTTTGGACAAATGGGAAGAGAACATTATTACATGTATAAAAAATTTGGAATT
    TGGCCTGAAAAAGAATATGCCATAAATTGGGTAAAAAAAATAAAAGATTATGTTAAAGAG
    CAAGATGAAGCTGAACTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    501

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0295 [new locus tag: SACOL_RS01495 ]
  • symbol: SACOL0295
  • description: hypothetical protein
  • length: 166
  • theoretical pI: 4.13341
  • theoretical MW: 19989.2
  • GRAVY: -0.668675

⊟Function[edit | edit source]

  • TIGRFAM:
    conserved hypothetical protein (TIGR01741; HMM-score: 241.9)
  • TheSEED  :
    • Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
    Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
  • PFAM:
    no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 168.8)
    and 2 more
    Gemini_AL1_M; Geminivirus rep protein central domain (PF08283; HMM-score: 13.3)
    DUF1031; Protein of unknown function (DUF1031) (PF06275; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007829
    • TAT(Tat/SPI): 0.000381
    • LIPO(Sec/SPII): 0.000661
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFFNYTKPGSEDLNYYTDIPKEYNVSVQVFDDLWMDLYDLFKNLRNLFKEEGLEPWTSCEFDFTRDGKLNVSFDYIDWANSEFGQMGREHYYMYKKFGIWPEKEYAINWVKKIKDYVKEQDEAEL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL0295&oldid=96487"