From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0325 [new locus tag: SACOL_RS01635 ]
  • pan locus tag?: SAUPAN001425000
  • symbol: SACOL0325
  • pan gene symbol?: —
  • synonym:
  • product: prophage L54a, antirepressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0325 [new locus tag: SACOL_RS01635 ]
  • symbol: SACOL0325
  • product: prophage L54a, antirepressor
  • replicon: chromosome
  • strand: +
  • coordinates: 358916..359707
  • length: 792
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCAAGCATTACAAACATTTAATTTTAAAGAGCTACCAGTAAGAACAGTAGAAATTGAA
    AACGAACCTTATTTTGTAGGAAAAGATATTGCTGAGATTTTAGGATATGCAAGATCAGAC
    AATGCCATTAGAAATCATGTTGATAGCGAGGACAAGCTGACGCACCAATTTAGTGCATCA
    GGTCAAAACAGAAATATGATCATTATCAACGAATCAGGATTATACAGTCTAATCTTCGAT
    GCTTCTAAACAAAGCAAAAACGAAAAAATCAGAGAAACCGCTCGGAAATTCAAACGATGG
    GTAACATCAGATGTCCTACCAGCTATTCGCAAACACGGTATATACGCAACAGACAATGTA
    ATTGAACAAACATTAAAAGATCCAGACTACATCATTACAGTGTTGACTGAGTATAAGAAA
    GAAAAAGAGCAAAACTTACTTTTACAACAAGAAATCGGAGAACTAAAACCCAAAGCAGAC
    TATGTAGATGAAATCTTAAAGTCAACTGGCACATTAGCCACAACTCAAATCGCGGCAGAC
    TACGGTATATCAGCACAAAAGTTAAACAAACTACTACACGAAGCTAGACTACAACGAAAA
    GTAAATAAACAGTGGGTGCTTTACTCAGAACACATGGGCAAGAGTTACACAGAATCAGAC
    ACTATAGCAATTGTACGCTCTGACGGTAGAGAAGACACAGTTTTACAAACTAGATGGACA
    CAAAAAGGCAGATTGAAAATACATGAAATCATGACTGAATTCGGTTATGAAGCTAATTTA
    GGGGGAGCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0325 [new locus tag: SACOL_RS01635 ]
  • symbol: SACOL0325
  • description: prophage L54a, antirepressor
  • length: 263
  • theoretical pI: 7.18406
  • theoretical MW: 30156.9
  • GRAVY: -0.601521

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Phage antirepressor protein
  • PFAM:
    no clan defined ANT; Phage antirepressor protein KilAC domain (PF03374; HMM-score: 101.6)
    Bro-N; BRO family, N-terminal domain (PF02498; HMM-score: 96)
    and 3 more
    P22_AR_N; P22_AR N-terminal domain (PF10547; HMM-score: 19.3)
    HTH (CL0123) AbiEi_4; Transcriptional regulator, AbiEi antitoxin (PF13338; HMM-score: 12.7)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004627
    • TAT(Tat/SPI): 0.000295
    • LIPO(Sec/SPII): 0.000424
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQALQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARSDNAIRNHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNEKIRETARKFKRWVTSDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTESDTIAIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL0325&oldid=97651"