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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0364 [new locus tag: SACOL_RS01830 ]
  • pan locus tag?:
  • symbol: SACOL0364
  • pan gene symbol?: —
  • synonym:
  • product: prophage L54a, RinA family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0364 [new locus tag: SACOL_RS01830 ]
  • symbol: SACOL0364
  • product: prophage L54a, RinA family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 372895..373332
  • length: 438
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGGAAAGGCATCATATGATATTAAGCCAGGTACATTTAAATATATTGAGTCAGAGATA
    TATAACCTACAAGAGAACAAGAAAGAGATAAATAGATTGAGAATGGAGATACTTAACCCA
    ACGAAAGAACTAGACACCAACATTGTGTATGGACCGTTACAAAAAGGAGAGCCAGTTAGA
    ACAACTGAGTTAATGGCGACAAGGTTATTGACTAATAAGATGTTACGTAACTTAGAAGAG
    ATGGTTGAAGCAGTTGAAAGTGAGTACTTAAAGTTACCTGAAGATCATAAGAAAGTAATA
    AGGTTAAAGTATTGGAATAAAGATAAGAAGCTAAAGATAGAACAAATAGGGGATGCTTGT
    CACATGCATCGCAATACAGTTACTACAATACGAAAGAACTTTGTTAAAGCGATAGCGTAT
    CATGCAGGTATCAAATAA
    60
    120
    180
    240
    300
    360
    420
    438

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0364 [new locus tag: SACOL_RS01830 ]
  • symbol: SACOL0364
  • description: prophage L54a, RinA family transcriptional regulator
  • length: 145
  • theoretical pI: 9.92748
  • theoretical MW: 17035.8
  • GRAVY: -0.686207

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage transcriptional regulator, RinA family (TIGR01636; HMM-score: 61.4)
    Signal transduction Regulatory functions DNA interactions phage transcriptional regulator, RinA family (TIGR01636; HMM-score: 61.4)
    and 2 more
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 19.8)
    RNA polymerase sigma-70 factor, Planctomycetaceae-specific subfamily 1 (TIGR02984; HMM-score: 14.2)
  • TheSEED  :
    • Phage integrase regulator RinA
  • PFAM:
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.3)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 15.2)
    no clan defined S-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 14.5)
    HTH (CL0123) HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 14.2)
    and 1 more
    no clan defined PDXDC1-like_cen2; PDXDC1-like second central domain (PF22937; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8807
    • Cytoplasmic Membrane Score: 0.0948
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.024
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005008
    • TAT(Tat/SPI): 0.000253
    • LIPO(Sec/SPII): 0.000374
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKASYDIKPGTFKYIESEIYNLQENKKEINRLRMEILNPTKELDTNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRLKYWNKDKKLKIEQIGDACHMHRNTVTTIRKNFVKAIAYHAGIK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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