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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0477 [new locus tag: SACOL_RS02415 ]
  • pan locus tag?: SAUPAN002122000
  • symbol: SACOL0477
  • pan gene symbol?: hsdS1
  • synonym:
  • product: type I restriction-modification system S subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0477 [new locus tag: SACOL_RS02415 ]
  • symbol: SACOL0477
  • product: type I restriction-modification system S subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 478598..479809
  • length: 1212
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGTAATACACAAAAGAAAAATGTGCCAGAATTGAGGTTCCCAGGGTTTGAAGGCGAA
    TGGGAAGAGAAGCAGTTAGGGGATCTTACAGATAGAGTAATTAGGAAAAATAAAAACTTA
    GAATCGAAAAAGCCTTTAACAATATCCGGACAGTTAGGTTTAATTGATCAAACAGAATAT
    TTTAGTAAATCAGTTTCGTCGAAAAATCTAGAAAATTATACACTAATAAAGAATGGAGAA
    TTCGCGTATAACAAAAGTTATTCTAATGGATACCCATTAGGGGCTATTAAAAGATTAACT
    AGATATGATAGTGGTGTATTGTCCTCTTTGTATATTTGTTTTTCTATTAAAAGTGAAATG
    TCTAAAGACTTCATGGAAGCATATTTTGATTCGACACACTGGTATAGAGAAGTTTCTGGA
    ATTGCAGTTGAGGGTGCAAGAAATCACGGATTATTAAATGTTTCTGTGAATGATTTTTTT
    ACTATTCTAATTAAATATCCAAGTTTAGAAGAACAGCAAAAAATAGGCAAGTTCTTCAGC
    AAACTCGACCGACAAATTGAATTAGAAGAACAAAAGCTTGAATTACTTCAACAACAGAAA
    AAAGGCTATATGCAGAAAATTTTCTCACAGGAACTGCGATTCAAAGATGAGAATGGTGAA
    GATTATCCAGATTGGGAAAATAGCAAAATAGAAAAATATTTAAAAGAGAGAAACGAACGT
    TCTGACAAAGGGCAAATGCTTTCAGTAACTATAAATAGTGGCATTATAAAATTTAGTGAA
    TTGGATAGAAAAGATAATTCAAGTAAAGATAAAAGTAATTATAAAGTAGTTAGGAAAAAT
    GATATTGCATATAATTCTATGAGAATGTGGCAAGGGGCTAGTGGTAAATCAAATTATAAT
    GGGATTGTTAGCCCTGCATATACTGTGCTTTATCCAACACAAAATACTAGCTCATTATTT
    ATTGGATATAAGTTTAAAACACATAGAATGATTCATAAATTTAAAATTAATTCACAAGGA
    TTAACATCAGATACATGGAACTTAAAATATAAACAATTAAAAAATATAAATATAGATATA
    CCTGTATTGGAGGAACAAGAAAAGATAGGTGATTTCTTTAAAAAAATGGATATATTGATA
    AGTAAACAGAAAATGAAAATTGAAATATTAGAAAAAGAGAAACAATCCTTTTTACAAAAA
    ATGTTCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0477 [new locus tag: SACOL_RS02415 ]
  • symbol: SACOL0477
  • description: type I restriction-modification system S subunit
  • length: 403
  • theoretical pI: 9.74077
  • theoretical MW: 47135.4
  • GRAVY: -0.74665

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 12.2)
  • TheSEED  :
    • Type I restriction-modification system, specificity subunit S
    DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
    and 1 more
    DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)
  • PFAM:
    TRD (CL0477) Methylase_S; Type I restriction modification DNA specificity domain (PF01420; HMM-score: 55.2)
    and 1 more
    no clan defined Jag_N; Jag N-terminus (PF14804; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00574
    • TAT(Tat/SPI): 0.000973
    • LIPO(Sec/SPII): 0.00056
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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