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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0574 [new locus tag: SACOL_RS02980 ]
- pan locus tag?: SAUPAN002294000
- symbol: gltX
- pan gene symbol?: gltX
- synonym:
- product: glutamyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0574 [new locus tag: SACOL_RS02980 ]
- symbol: gltX
- product: glutamyl-tRNA synthetase
- replicon: chromosome
- strand: +
- coordinates: 596014..597468
- length: 1455
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238712 NCBI
- RefSeq: YP_185460 NCBI
- BioCyc: see SACOL_RS02980
- MicrobesOnline: 912054 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGGTATCTTCATATTGGT
AATGCAAGAACAGCATTATTCAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTG
ATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAGATGGAGAAACATCACAATTT
GATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTA
GCAGAAGATAAAGCATATAAATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAA
GCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTCAACATGCGCATTTGACTGAA
GAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAAT
GGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTA
GCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCA
AACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATT
TTACAATTTATTGAGCAATATCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTT
ATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAATCTTTTCTAAAGAAGAATTT
ATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTA
GCATTACCTCATTTAATTAAAGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTA
TCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAATGAGTTATGCCGGTGAAATT
GTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
GTGATTAATGGAAAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCA
CTTGAACCATTTGAATCGGCTGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACA
GGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTGCTGTAACAGGCCAAATGCAT
GGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
AAACAATATAAGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0574 [new locus tag: SACOL_RS02980 ]
- symbol: GltX
- description: glutamyl-tRNA synthetase
- length: 484
- theoretical pI: 5.09349
- theoretical MW: 56303.5
- GRAVY: -0.645868
⊟Function[edit | edit source]
- reaction: EC 6.1.1.17? ExPASyGlutamate--tRNA ligase ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
- TIGRFAM: Protein synthesis tRNA aminoacylation glutamate--tRNA ligase (TIGR00464; EC 6.1.1.17; HMM-score: 624.4)and 4 moreProtein synthesis tRNA and rRNA base modification glutamyl-queuosine tRNA(Asp) synthetase (TIGR03838; EC 6.1.1.-; HMM-score: 219.3)Protein synthesis tRNA aminoacylation glutamate--tRNA ligase (TIGR00463; EC 6.1.1.17; HMM-score: 127.9)Protein synthesis tRNA aminoacylation glutamine--tRNA ligase (TIGR00440; EC 6.1.1.18; HMM-score: 52.3)Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 27.2)
- TheSEED :
- tRNA glutamyl-Q(34)-Gln synthetase (EC 6.1.1.24)
- tRNA glutamyl-Q(34)-Glu synthetase (EC 6.1.1.17)
Protein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln Glutamyl-tRNA synthetase (EC 6.1.1.17)and 3 moreProtein Metabolism Protein biosynthesis tRNA aminoacylation, Glu and Gln Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24) - PFAM: HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 414.3)and 2 moretRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.7)no clan defined Mrr_N; Mrr N-terminal domain (PF14338; HMM-score: 12.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003965
- TAT(Tat/SPI): 0.000323
- LIPO(Sec/SPII): 0.000767
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGKQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: 36.92 h [6]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)