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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0598 [new locus tag: SACOL_RS03100 ]
  • pan locus tag?: SAUPAN002324000
  • symbol: SACOL0598
  • pan gene symbol?: araB
  • synonym:
  • product: ribulokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0598 [new locus tag: SACOL_RS03100 ]
  • symbol: SACOL0598
  • product: ribulokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 623461..625098
  • length: 1638
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTATAGCATTGGAATTGATTATGGAACTGCTTCAGGCCGTGTGTTTTTAATTAAT
    ACAACTAACGGTCAAGTAGTATCAAAATTTGTGAAGCCATATACACATGGTGTCATTGAG
    AGTGAATTAAATGGTTTGAAAATACCACATACATATGCACTTCAAAATAGTAATGATTAT
    TTAGAAATTATGGAAGAAGGAATATCATATATAGTACGTGAATCAAAAATAGATCCAGAC
    AATATAGTAGGTATTGGTATAGACTTTACTTCATCTACTATTATTTTTACTGACGAAAAC
    CTTAACCCGGTACATAACTTAAAACAATTTAAAAACAATCCACATGCGTATGTGAAACTT
    TGGAAACATCATGGTGCATATAAAGAGGCAGAGAAATTATATCAAACTGCTATTGAAAAT
    AATAATAAGTGGTTAGGCCATTATGGATATAATGTTAGTAGTGAATGGATGATTCCCAAA
    ATAATGGAGGTCATGAATCGAGCACCAGAAATTATGGAAAAAACGGCTTATATTATGGAA
    GCGGGCGATTGGATTGTAAATAAATTAACTAATAAAAATGTACGCTCGAATTGTGGATTA
    GGTTTCAAAGCATTTTGGGAAGAAGAAACAGGGTTTCATTATGATTTATTTGATAAAATA
    GACCCCAAATTATCAAAAGTAATTCAAGATAAAGTATCTGCACCGGTTGTTAATATTGGT
    GAAGCAGTAGGGAAACTGGATGATAAAATGGCACAGAAATTAGGATTATCAAAAGAAACT
    ATGGTAAGTCCTTTTATTATTGATGCCCATGCTAGTTTATTAGGTATTGGGTCTGAAAAA
    GATAAAGAAATGACTATGGTGATGGGAACAAGCACATGCCATCTTATGTTAAATGAAAAG
    CAACATCAAGTGCCAGGTATATCAGGTTCTGTAAAAGGAGCAATTATTCCAGAATTATTT
    GCTTATGAAGCGGGGCAATCAGCAGTAGGTGATTTGTTTGAGTATGTCGCTAAGCAAGCA
    CCAAAGTCATATGTAGATGAAGCAGAAAATAGAAATATGACTGTATTTGAATTAATGAAT
    GAAAAGATAAAACATCAAATGCCAGGTGAAAGTGGGCTCATTGCTCTTGATTGGCATAAT
    GGAAATCGAAGTGTATTAAGTGATAGCAATTTAACAGGTTGTATCTTTGGATTAACTTTA
    CAAACTAAGCATGAGGATATTTATAGAGCATATTTAGAAGCTACAGCATTTGGTACTAAG
    ATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGC
    GGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAA
    CTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTC
    AGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTA
    TACGAAATTAATCCAGAAGCGGAAAAAGTACAAAGGTATGAAACATTATTTAAAGCTTAT
    AAGGCTTTACATGATATCCATGGTTATAAAAAAGCTAATATAATGAAAGATATCCAGAGT
    TTAAGAGTTGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0598 [new locus tag: SACOL_RS03100 ]
  • symbol: SACOL0598
  • description: ribulokinase
  • length: 545
  • theoretical pI: 6.04212
  • theoretical MW: 61010.4
  • GRAVY: -0.278165

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.16?  ExPASy
    Ribulokinase ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 557)
    and 5 more
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 209.7)
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 154.3)
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 86.9)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 68.4)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 58.6)
  • TheSEED  :
    • Ribulokinase (EC 2.7.1.16)
  • PFAM:
    Actin_ATPase (CL0108) FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 126)
    and 4 more
    FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 63.3)
    no clan defined DUF4846; Domain of unknown function (4846) (PF16138; HMM-score: 17.5)
    Actin_ATPase (CL0108) BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 13.3)
    Concanavalin (CL0004) SPRY; SPRY domain (PF00622; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.64
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013869
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.004046
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELNGLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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