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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0644 [new locus tag: SACOL_RS03325 ]
  • pan locus tag?: SAUPAN002446000
  • symbol: SACOL0644
  • pan gene symbol?: tsaI1
  • synonym: tsaI
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0644 [new locus tag: SACOL_RS03325 ]
  • symbol: SACOL0644
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 675514..676140
  • length: 627
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGCTTTTTAATATTAAGGTTATTAATAAAAATCCGAGATATAAAGTCGTTCAATATAAT
    GATGAGTATTTGTTAATTGATTTAGTAAGCACTTGGCTTGTATACTTTTTTCCTTTTATT
    AATTGGTTCATTCCCAAAAGGTATGCAAAACTTAGTGAGAAGGAACTTGAAAATTTAAAT
    GTTGATAAACAAAATAAAAATAATATTTTCTGGCCAGTTACTGGTAGTTCGTTTTTATTT
    GTAGTTATTTTACGGAAATATGTACATACGTTTGAAGTTCAATTAGATAATAAGATTTTA
    ATATCTTTATGTTTTATAGGATTTATAGGTATTGCAGCATTTTACATTTACTTAAATAAA
    AAGCTTAAACTAAAGATATATGATGATAATCTAGATAATGAAAATAGGGTTATATTAGTG
    CCTACTTTTAAAGATGGAAGTTTTATAGTTTTCACATATCTATTATTAGGAGGTTGTTCT
    ATATTATTTTTAATATGGCTAATGACTATAAAACCTCAAAATCTACTGGTATTTATTATG
    TGGATTATTATTACAATTTTTTTCTTTCTAATAAGCATGGGCTCAATTAGTAATAAAAAG
    GTTTATGCCAAACTTAAAAAGCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0644 [new locus tag: SACOL_RS03325 ]
  • symbol: SACOL0644
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 10.0279
  • theoretical MW: 24689.4
  • GRAVY: 0.448077

⊟Function[edit | edit source]

  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 185.2)
  • TheSEED  :
    • repetative hypothetical protein , fig|282458.1.peg.574 homolog
  • PFAM:
    no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 189.5)
    and 3 more
    DUF4191; Domain of unknown function (DUF4191) (PF13829; HMM-score: 13.5)
    ClpS; ATP-dependent Clp protease adaptor protein ClpS (PF02617; HMM-score: 13.3)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 9.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001808
    • TAT(Tat/SPI): 0.000124
    • LIPO(Sec/SPII): 0.00041
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLFNIKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFIPKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFVVILRKYVHTFEVQLDNKILISLCFIGFIGIAAFYIYLNKKLKLKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCSILFLIWLMTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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