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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0709 [new locus tag: SACOL_RS03645 ]
  • pan locus tag?: SAUPAN002530000
  • symbol: SACOL0709
  • pan gene symbol?: dhaM
  • synonym:
  • product: phosphotransferase mannnose-specific family component IIA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0709 [new locus tag: SACOL_RS03645 ]
  • symbol: SACOL0709
  • product: phosphotransferase mannnose-specific family component IIA
  • replicon: chromosome
  • strand: +
  • coordinates: 734272..734634
  • length: 363
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCCTAAAATTATACTTGTTAGCCACAGTAAAGAAATTGCAAGTGGTACAAAATCTTTG
    TTAAAGCAAATGGCAGGTGACGTTGATATTATACCAATCGGGGGATTACCAGATGGTTCA
    ATTGGAACTTCATTTGATATCATCCAAGAAGTTTTGACTAAATTAGAGGATGATGCATTG
    TGTTTTTACGATATTGGATCTTCAGAAATGAATGTAGATATGGCAATTGAAATGTATGAT
    GGTAATCATCGTGTGTTAAAAGTTGATGCACCAATTGTTGAAGGCAGTTTTATCGCAGCA
    GTAAAGCTATCAATCGGCGGTTCAATTGATGATGCATTAGCAGAAATCAAACAATCATTT
    TAG
    60
    120
    180
    240
    300
    360
    363

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0709 [new locus tag: SACOL_RS03645 ]
  • symbol: SACOL0709
  • description: phosphotransferase mannnose-specific family component IIA
  • length: 120
  • theoretical pI: 4.1342
  • theoretical MW: 12784.6
  • GRAVY: 0.265

⊟Function[edit | edit source]

  • TIGRFAM:
    dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 144.8)
  • TheSEED  :
    • DHA-specific IIA component
    • Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), subunit DhaM
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  DHA-specific IIA component
    and 1 more
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Phosphoenolpyruvate-dihydroxyacetone phosphotransferase (EC 2.7.1.121), subunit DhaM
  • PFAM:
    EDD (CL0245) EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 96.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007984
    • TAT(Tat/SPI): 0.000717
    • LIPO(Sec/SPII): 0.001066
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTSFDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVKLSIGGSIDDALAEIKQSF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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