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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0712 [new locus tag: SACOL_RS03660 ]
  • pan locus tag?: SAUPAN002533000
  • symbol: SACOL0712
  • pan gene symbol?: gehE
  • synonym:
  • product: lipase/esterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0712 [new locus tag: SACOL_RS03660 ]
  • symbol: SACOL0712
  • product: lipase/esterase
  • replicon: chromosome
  • strand: +
  • coordinates: 736921..737964
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATAAAGATAATAAATGGACGATGATAACTGCGCTTTTTATAACTGTAATCAGTGTA
    TTGTTAGCATTTCATCTGAAACAACATTATGACCAAATTACAAATGAGAACCATGCTAAT
    AAAGACAAAATTAATATTAAAAATAAAAATGTGCGCATTTATCAAAACCTTACATACAAT
    AGAGTTTTCCCTAACAGTAAATTAGATATTATTACACCTGTTGATATGTCTTCTAATGCC
    AAACTGCCAGTTATTTTTTGGATGCACGGTGGTGGTTATATTGCGGGTGATAAGCAGTAT
    AAAAACCCATTATTAGCGAAAATTGCTGAACAAGGGTACATTGTTGTGAATGTAAATTAT
    GCATTGGCGCCACAATATAAATATCCCACACCATTAATTCAAATGAATCAAGCAACTCAA
    TTCATTAAAGAAAATAAAATGAATTTACCTATTGATTTTAATCAAGTAATTATTGGCGGT
    GATTCTGCAGGTGCTCAATTAGCTAGCCAATTTACGGCAATACAGACGAATGATCGCTTA
    AGAGAAGCCATGAAATTTGATCAGTCATTCAAACCATCGCAAATTAAAGGTGCTATACTA
    TTTGGTGGTTTTTATAATATGCAAACAGTTAGAGAAACTGAGTTTCCAAGAATACAGTTA
    TTTATGAAAAGTTATACTGGCGAAGAAGATTGGGAAAAGAGTTTTAAAAACATTTCACAA
    ATGTCGACAGTAAAACAATCGACAAAAAATTATCCACCAACATTTTTATCTGTTGGAGAT
    AGCGATCCATTCGAAAGTCAAAATATAGAATTCAGTAAGAAATTACAAGAATTGAATGTA
    CCAGTAGATACTTTGTTTTATGATGGTACGCATCATTTACATCATCAGTATCAATTTCAC
    CTTAATAAACCTGAATCGATAGATAATATCAAAAAAGTGTTACTTTTCTTAAGTCGTAAT
    ACATCCTCTAGTGGTATTCAAACTGAAGAGAAACCACAAATAGAAAATCCGAGTAATGAA
    TTACCGTTAAATCCTTTAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0712 [new locus tag: SACOL_RS03660 ]
  • symbol: SACOL0712
  • description: lipase/esterase
  • length: 347
  • theoretical pI: 9.18823
  • theoretical MW: 39838.1
  • GRAVY: -0.503458

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.5)
  • TheSEED  :
    • Lipase LipA
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 110.8)
    and 7 more
    COesterase; Carboxylesterase family (PF00135; HMM-score: 37.8)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 25.7)
    Chlorophyllase2; Chlorophyllase enzyme (PF12740; HMM-score: 22.5)
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 17.2)
    Cutinase; Cutinase (PF01083; HMM-score: 15.3)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 13.6)
    PAF-AH_p_II; Platelet-activating factor acetylhydrolase, isoform II (PF03403; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.3
    • Cellwall Score: 4.55
    • Extracellular Score: 5.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.312091
    • TAT(Tat/SPI): 0.002085
    • LIPO(Sec/SPII): 0.025561
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKINIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGGDSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQIENPSNELPLNPLN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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