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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0714 [new locus tag: SACOL_RS03675 ]
- pan locus tag?: SAUPAN002536000
- symbol: SACOL0714
- pan gene symbol?: —
- synonym:
- product: acetyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0714 [new locus tag: SACOL_RS03675 ]
- symbol: SACOL0714
- product: acetyltransferase
- replicon: chromosome
- strand: -
- coordinates: 738930..739436
- length: 507
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236595 NCBI
- RefSeq: YP_185596 NCBI
- BioCyc: see SACOL_RS03675
- MicrobesOnline: 912192 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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481ATGGCCCATATTATACGTAGAGTTAGTATCAAAGATGTAGAAAATTTCATTTCAATGTTA
GCGAACATATACGACGAATCTCCGTATATGTTCTACACACCAGGAGAATATGATCCTAGC
GTCACATCGGCTAGTAAACAATTAGAAGAATATATCACTTCTCCGCATAAAGTCATCTTC
GTTGCTGAAAGTGATGAACAACTCGTTGGCTTTGCCTTTGTTAATACGACACCATTTCAA
CGCATTAAACATGTTGCTAAAATTGATTTAGGTGTAAAGAAATTATATCAACATCGTGGA
ATTGGCCAAGCACTTCTTGATGCCATTATGGCTTGGTGTTTAAACAATCAAATACACCGA
ATTGAAGCAAATGTACCACTCAATAACCAACCTGCCCTCGAGCTTTTTAAAAGTGCCGAT
TTTCAAATCGAAGGCGTTTTAAAAGATAAGTTATTTATCGATGGTAAATATTATGATGAC
TATATGATGGCTAAAATTCTTAATTAA60
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507
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0714 [new locus tag: SACOL_RS03675 ]
- symbol: SACOL0714
- description: acetyltransferase
- length: 168
- theoretical pI: 6.0027
- theoretical MW: 19279.1
- GRAVY: -0.129167
⊟Function[edit | edit source]
- TIGRFAM: Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 51.8)and 4 moreUDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase (TIGR03585; EC 2.3.1.202; HMM-score: 39.7)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs mycothiol synthase (TIGR03448; EC 2.3.1.189; HMM-score: 22.1)Cellular processes Adaptations to atypical conditions diaminobutyrate acetyltransferase (TIGR02406; EC 2.3.1.178; HMM-score: 20.7)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides TDP-D-fucosamine acetyltransferase (TIGR02382; HMM-score: 18.6)
- TheSEED :
- Acetyltransferase GNAT family
- PFAM: Acetyltrans (CL0257) Acetyltransf_4; Acetyltransferase (GNAT) domain (PF13420; HMM-score: 61.4)Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 60.4)and 8 moreAcetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 39.8)Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 37.3)Acetyltransf_3; Acetyltransferase (GNAT) domain (PF13302; HMM-score: 24.6)Acetyltransf_8; Acetyltransferase (GNAT) domain (PF13523; HMM-score: 18)no clan defined Hypoth_Ymh; Protein of unknown function (Hypoth_ymh) (PF09509; HMM-score: 14)Acetyltrans (CL0257) FR47; FR47-like protein (PF08445; HMM-score: 13.5)Acetyltransf_9; Acetyltransferase (GNAT) domain (PF13527; HMM-score: 12.8)DUF3749; Acetyltransferase (GNAT) domain (PF12568; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003266
- TAT(Tat/SPI): 0.000479
- LIPO(Sec/SPII): 0.000472
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSASKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIGQALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDDYMMAKILN
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 151 [4]
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: 54.26 h [5]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)