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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0733 [new locus tag: SACOL_RS03765 ]
  • pan locus tag?: SAUPAN002556000
  • symbol: SACOL0733
  • pan gene symbol?:
  • synonym:
  • product: sugar efflux transporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0733 [new locus tag: SACOL_RS03765 ]
  • symbol: SACOL0733
  • product: sugar efflux transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 756783..758003
  • length: 1221
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATATGTTTGCAGCGTTATTACAAATAAAGAATTATAAACTCTTTGTTGCTAATATG
    TTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAA
    GATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAG
    TTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATT
    ATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGAT
    ACAATCTGGTTATTCATATTACTATATGCGATTTTCCAAGGATTATTTGCACCAGCAATG
    CCCCAACTTTACGCATCTGCTAGAGAATCTATCAATGTTTCAAGCTCTAAAGATAGAGCT
    CAATTTGCCAACACAGTATTACGTTCAATGTTCTCATTGGGCTTTTTATTTGGTCCATTT
    ATTGGTGCCCAATTAATCGGATTAAAAGGCTATGCTGGATTGTTTGGTGGAACAATAAGT
    ATCATTTTATTTACTTTAGTACTTCAAGTGTTTTTCTATAAGGATTTAAACATTAAACAC
    CCTATTAGTACGCAACAACATGTTGAAAAAATTGCTCCTAATATGTTTAAAGACAAAACG
    CTTTTATTACCATTTATTGCATTTATTTTATTACACATTGGACAATGGATGTATACGATG
    AATATGCCTTTATTTGTTACTGATTATTTAAAAGAAAATGAACAACATGTCGGTTATTTA
    GCTAGTTTATGTGCTGGTTTAGAAGTGCCATTTATGATCATTCTTGGCGTTTTATCATCT
    AGATTACAGACTCGAACATTGTTGATTTATGGAGCGATTTTTGGTGGTTTATTCTACTTC
    AGCATTGGGGTATTTAAAAACTTCTATATGATGTTAGCAGGACAGGTGTTTTTAGCTATT
    TTCTTAGCGGTTCTTTTAGGAATTGGTATTAGTTATTTCCAAGATATCTTACCAGATTTT
    CCAGGATACGCTTCAACACTATTTTCTAATGCAATGGTTATTGGACAGTTAGGCGGTAAC
    CTATTAGGTGGTGCTATGAGTCACTGGGTAGGTTTGGAAAATGTATTTTTTGTATCAGCA
    GCATCAATCATGTTAGGTATGATACTTATATTCTTTACTAAAAATCAAAAAATTACAAAA
    GAGGATGTGATATCAACATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1221

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0733 [new locus tag: SACOL_RS03765 ]
  • symbol: SACOL0733
  • description: sugar efflux transporter
  • length: 406
  • theoretical pI: 9.39681
  • theoretical MW: 45130.5
  • GRAVY: 0.813054

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar efflux transporter (TIGR00899; HMM-score: 161.2)
    and 5 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 44.8)
    multidrug resistance protein (TIGR00880; HMM-score: 41.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 17.1)
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 4)
  • TheSEED:  
    Sugar efflux transpoter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 120.4)
    and 3 more
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 29.5)
    ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 17.5)
    no clan defined Ilm1; Increased loss of mitochondrial DNA protein 1 (PF10311; HMM-score: 12.1)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016845
    • TAT(Tat/SPI): 0.00127
    • LIPO(Sec/SPII): 0.006278
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGMTTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILGVLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFFTKNQKITKEDVIST

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]