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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1028 [new locus tag: SACOL_RS05240 ]
  • pan locus tag?: SAUPAN003203000
  • symbol: htrA
  • pan gene symbol?: htrA2
  • synonym:
  • product: serine protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1028 [new locus tag: SACOL_RS05240 ]
  • symbol: htrA
  • product: serine protease
  • replicon: chromosome
  • strand: +
  • coordinates: 1036719..1039028
  • length: 2310
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    GTGGATATTGGTAAAAAACATGTAATTCCTAAAAGTCAGTACCGACGTAAGCGTCGTGAA
    TTCTTCCACAACGAAGACAGAGAAGAAAATTTAAATCAACATCAAGATAAACAAAATATA
    GATAATACAACATCAAAAAAAGCAGATAAGCAAATACATAAAGATTCAATTGATAAGCAC
    GAACGTTTTAAAAATAGTTTATCATCGCATTTAGAACAGAGAAACCGTGATGTTAATGAG
    AATAAAGCTGAAGAAAGTAAAAGTAATCAGGATAGTAAGTCAGCATATAACAGAGATCAT
    TATTTAACAGACGATGTATCTAAAAAACAAAATTCATTAGATTCAGTGGACCAAGATACA
    GAGAAATCAAAATATTATGAGCAAAATTCTGAAGCGACTTTATCAACTAAATCAACCGAT
    AAAGTAGAATCAACTGAAATGAGAAAGCTAAGTTCAGATAAAAACAAAGTTGGTCATGAA
    GAGCAACATGTACTTTCTAAACCTTCAGAACATGATAAAGAGACTAGAATTGATTCTGAG
    TCTTCAAGAACTGATTCAGACAGCTCGATGCAGACAGAGAAAATAAAAAAAGACAGTTCA
    GATGGAAATAAAAGTAGTAATCTGAAATCTGAAGTAATATCAGACAAATCAAATACAGTA
    CCAAAATTGTCGGAATCTGATGATGAAGTAAATAATCAGAAGCCATTAACTTTACCGGAA
    GAACAGAAATTGAAAAGACAGCAAAGTCAAAATGAGCAAACAAAAACCTATACATATGGT
    GATAGCGAACAAAATGACAAGTCTAATCATGAAAATGATTTAAGTCATCATATACCATCG
    ATAAGTGATGATAAAGATAACGTCATGAGAGAAAATCATATTGTTGACGATAATCCTGAT
    AATGATATCAATACACCATCATTATCAAAAACAGATGACGATCGAAAACTTGATGAAAAA
    ATTCATGTTGAAGATAAACATAAACAAAATGCAGACTCGTCTGAAACGGTGGGATATCAA
    AGTCAGTCAACTGCATCTCATCGTAGCACTGAAAAAAGAAATATTTCTATTAATGACCAT
    GATAAATTAAACGGTCAAAAAACAAATACAAAGACATCGGCAAATAATAATCAAAAAAAG
    GCTACATCAAAATTGAACAAAGGGCGCGCTACGAATAATAATTATAGTGACATTTTGAAA
    AAGTTTTGGATGATGTATTGGCCTAAATTAGTTATTCTAATGGGTATTATTATTCTAATT
    GTTATTTTGAATGCCATTTTTAATAATGTGAACAAAAATGATCGCATGAATGATAATAAT
    GATGCAGATGCTCAAAAATATACGACAACGATGAAAAATGCCAATAACACAGTTAAATCG
    GTCGTTACAGTTGAAAATGAAACATCAAAAGATTCATCATTACCTAAAGATAAAGCATCT
    CAAGACGAAGTGGGATCAGGTGTTGTATATAAAAAATCTGGAGATACGTTATATATTGTT
    ACGAATGCACACGTTGTCGGTGATAAAGAAAATCAAAAAATAACTTTCTCGAATAATAAA
    AGTGTTGTTGGGAAAGTGCTTGGTAAAGATAAATGGTCAGATTTAGCTGTTGTTAAAGCA
    ACTTCTTCAGACAGTTCAGTGAAAGAGATAGCTATTGGAGATTCAAATAATTTAGTGTTA
    GGAGAGCCAATATTAGTCGTAGGTAATCCACTTGGTGTAGACTTTAAAGGCACTGTGACA
    GAAGGTATTATTTCAGGTCTGAACAGAAATGTTCCTATTGATTTCGATAAAGATAATAAA
    TATGATATGTTGATGAAAGCTTTCCAAATTGATGCATCAGTAAATCCAGGTAACTCGGGT
    GGTGCTGTCGTCAATAGAGAAGGAAAATTAATAGGTGTAGTTGCAGCTAAAATTAGTATG
    CCAAACGTTGAAAATATGTCATTTGCAATACCTGTTAATGAAGTACAAAAGATTGTAAAA
    GATTTAGAAACAAAAGGTAAAATTGACTATCCCGATGTAGGTGTTAAAATGAAGAATATT
    GCCAGTCTAAATAGTTTTGAAAGACAAGCAGTTAAATTGCCAGGAAAAGTTAAGAACGGT
    GTTGTTGTAGATCAAGTTGACAACAATGGTTTAGCAGATCAATCTGGTCTGAAAAAAGGT
    GATGTAATTACTGAATTAGATGGCAAACTTTTAGAAGATGATTTACGCTTTAGGCAGATT
    ATATTTAGTCATAAAGATGACTTGAAATCAATTACAGCGAAGATTTATAGAGATGGTAAA
    GAGAAAGAAATTAATATTAAACTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2310

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1028 [new locus tag: SACOL_RS05240 ]
  • symbol: HtrA
  • description: serine protease
  • length: 769
  • theoretical pI: 6.98599
  • theoretical MW: 86431.1
  • GRAVY: -1.03914

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 219.6)
    and 3 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.5)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 139.5)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 17.9)
  • TheSEED  :
    • Serine protease, DegP/HtrA, do-like (EC 3.4.21.-)
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 106.9)
    and 6 more
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 43.7)
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 42.8)
    PDZ-like (CL0466) PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 22.5)
    no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 15.1)
    Peptidase_PA (CL0124) Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 13.9)
    no clan defined DUF3671; Protein of unknown function (PF12420; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007488
    • TAT(Tat/SPI): 0.001415
    • LIPO(Sec/SPII): 0.001688
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQNIDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYNRDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKNKVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVISDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHENDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQNADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNKGRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQKYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNAHVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLGEPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKMKNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDLRFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cell wall associated [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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