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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1315 [new locus tag: SACOL_RS06705 ]
  • pan locus tag?: SAUPAN003600000
  • symbol: hexA
  • pan gene symbol?: mutS
  • synonym:
  • product: DNA mismatch repair protein MutS

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1315 [new locus tag: SACOL_RS06705 ]
  • symbol: hexA
  • product: DNA mismatch repair protein MutS
  • replicon: chromosome
  • strand: +
  • coordinates: 1330455..1333073
  • length: 2619
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTAATGTTACACCAATGATGCAGCAATATTTAAAAATAAAATCAGAATACCAAGAT
    TGCTTATTATTTTTTAGACTAGGTGATTTCTATGAAATGTTTTATGAAGATGCCAAGGAG
    GCATCACGTGTACTTGAAATTACTTTAACTAAAAGAGATGCTAAAAAAGAAAATCCAATT
    CCGATGTGTGGTGTTCCGTATCATTCTGCAGATAGTTATATAGATACACTTGTTAATAAT
    GGATATAAAGTAGCTATTTGTGAACAGATGGAAGATCCGAAACAAACGAAAGGTATGGTT
    AGACGTGAGGTAGTAAGAATTGTGACTCCAGGAACTGTGATGGAGCAAGGTGGTGTAGAT
    GATAAACAAAATAACTATATTTTAAGTTTTGTTATGAATCAACCTGAAATTGCGCTTAGT
    TACTGTGATGTTTCTACTGGCGAATTAAAGGTTACACATTTTAATGATGAAGCGACTTTA
    TTAAATGAAATTACGACGATAAACCCTAACGAAGTTGTTATCAATGACAATATTTCCGAT
    AATTTAAAAAGACAAATTAATATGGTGACAGAAACAATAACAGTCAGGGAAACGTTATCA
    TCAGAAATCTATAGTGTGAATCAAACTGAACATAAATTAATGTATCAAGCGACACAATTA
    TTGCTAGATTATATTCATCATACACAAAAACGTGATTTATCGCATATCGAGGATGTTGTT
    CAATATGCAGCTATAGATTATATGAAAATGGATTTTTATGCTAAGAGAAACCTTGAGTTA
    ACGGAAAGCATTCGATTAAAATCAAAAAAAGGAACGCTACTTTGGCTAATGGACGAAACG
    AAAACACCAATGGGAGCACGCCGCTTAAAACAATGGATAGATAGACCACTAATAAGTAAA
    GAACAAATTGAAGCACGATTAGATATCGTTGATGAATTTAGTGCTCATTTCATAGAAAGA
    GACACCTTAAGAACATATCTTAATCAAGTGTATGATATTGAACGTCTTGTTGGGCGTGTT
    AGTTACGGAAATGTTAATGCGAGAGATTTAATTCAACTTAAACATTCCATTTCTGAAATA
    CCGAATATTAAAGCATTACTAAATTCTATGAATCAGAATACTCTTGTACAAGTTAATCAA
    CTAGAACCCCTTGATGATTTACTTGATATATTAGAACAGAGTTTAGTAGAAGAACCACCA
    ATTTCAGTTAAAGATGGCGGACTATTCAAAGTTGGTTTTAATACGCAATTAGATGAATAT
    CTTGAAGCTTCAAAAAACGGAAAAACATGGTTAGCAGAATTACAAGCCAAAGAAAGACAA
    CGTACAGGAATAAAATCATTGAAAATAAGCTTTAATAAAGTGTTTGGTTATTTTATAGAA
    ATAACACGTGCCAACTTGCAAAATTTTGAACCAAGTGAATTTGGTTATATGAGGAAGCAA
    ACGTTATCGAATGCTGAACGTTTTATAACTGATGAACTTAAAGAAAAAGAAGATATCATT
    TTAGGTGCGGAAGACAAAGCCATCGAATTAGAATATCAATTATTTGTTCAGCTACGTGAA
    GAAGTTAAAAAATATACTGAACGTTTACAACAACAAGCTAAAATTATTTCAGAGCTAGAT
    TGTTTACAGAGCTTTGCAGAAATTGCTCAAAAATATAATTACACTAGGCCTTCATTTAGT
    GAAAATAAAACATTAGAATTAGTGGAATCTAGGCACCCAGTAGTGGAAAGAGTAATGGAT
    TATAATGACTATGTGCCTAATAATTGTCGATTAGATAATGAAACATTTATATATTTAATT
    ACAGGTCCGAATATGTCTGGTAAATCGACATATATGAGACAAGTTGCCATAATTAGTATA
    ATGGCCCAAATGGGAGCTTATGTCCCTTGTAAAGAGGCAGTGTTACCTATATTTGATCAA
    ATATTCACTAGAATAGGTGCGGCAGATGATTTGGTTTCAGGTAAGAGTACGTTTATGGTA
    GAAATGCTAGAAGCACAAAAGGCATTAACTTATGCAACAGAGGATAGTTTGATTATTTTC
    GATGAAATTGGACGTGGTACTTCAACGTATGACGGTTTAGCTTTAGCGCAGGCAATGATA
    GAGTATGTAGCTGAAACATCACATGCTAAAACGTTATTTTCAACACATTATCATGAATTG
    ACAACATTAGATCAAGCATTACCAAGTCTAAAAAATGTTCACGTCGCTGCTAATGAATAT
    AAAGGTGAACTTATATTCTTGCATAAAGTCAAAGATGGTGCAGTTGACGATAGTTATGGT
    ATTCAAGTTGCGAAATTAGCTGATTTACCTGAAAAAGTTATTAGCAGAGCACAAGTGATT
    CTAAGCGAGTTTGAAGCGTCTGCTGGTAAAAAATCATCGATATCAAATTTAAAAATGGTC
    GAAAATGAACCTGAAATTAATCAAGAAAATTTAAACTTAAGTGTTGAAGAAACAACTGAT
    ACTTTATCTCAAAAAGACTTTGAACAAGCATCATTTGATTTGTTTGAAAATGATCAAGAA
    AGCGAGATTGAACTACAAATTAAAAATTTGAATTTATCTAATATGACACCAATTGAGGCA
    TTGGTGAAGTTAAGTGAATTACAAAATCAATTAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2619

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1315 [new locus tag: SACOL_RS06705 ]
  • symbol: HexA
  • description: DNA mismatch repair protein MutS
  • length: 872
  • theoretical pI: 4.62947
  • theoretical MW: 99903
  • GRAVY: -0.376032

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutS (TIGR01070; HMM-score: 1090.7)
    and 1 more
    Genetic information processing DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 147.8)
  • TheSEED  :
    • DNA mismatch repair protein MutS
    DNA Metabolism DNA repair DNA repair, bacterial MutL-MutS system  DNA mismatch repair protein MutS
  • PFAM:
    P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 275.6)
    and 8 more
    no clan defined MutS_I; MutS domain I (PF01624; HMM-score: 164.3)
    MutS_III; MutS domain III (PF05192; HMM-score: 151.2)
    MutS_IV; MutS family domain IV (PF05190; HMM-score: 96.5)
    MutS_II; MutS domain II (PF05188; HMM-score: 65.1)
    P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.4)
    AAA_14; AAA domain (PF13173; HMM-score: 16.6)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 11.3)
    AAA_23; AAA domain (PF13476; HMM-score: 9.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002012
    • TAT(Tat/SPI): 0.000216
    • LIPO(Sec/SPII): 0.000358
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRVLEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRREVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATLLNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQATQLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWLMDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIERLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILEQSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKISFNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAIELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLELVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQMGAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDEIGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEYKGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLKMVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMTPIEALVKLSELQNQLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 274 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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