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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1382 [new locus tag: SACOL_RS07035 ]
  • pan locus tag?: SAUPAN003722000
  • symbol: sbcC
  • pan gene symbol?: sbcC
  • synonym:
  • product: exonuclease SbcC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1382 [new locus tag: SACOL_RS07035 ]
  • symbol: sbcC
  • product: exonuclease SbcC
  • replicon: chromosome
  • strand: +
  • coordinates: 1384730..1387759
  • length: 3030
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAACCATTACATTTAAAGTTGAATAATTTCGGCCCCTTTTTAAAAGAAGAAATTGAT
    TTTTCTAAAATTGATAATAATGAATTGTTTTTAATAAGTGGTAAGACTGGATCGGGTAAA
    ACAATGATTTTTGATGCAATGACTTATGCCTTGTTTGGTAAAGCATCAACTGAACAAAGA
    GAAGAAAATGATTTGAGAAGTCATTTCGCTGATGGTAAACAGCCGATGTCAGTAACATTT
    GAATTTCAATTAAATCATCGAATTTATAAAGTGCATAGACAAGGCCCTTATATCAAAGAA
    GGTAATACAACAAAAACGAACGCTAAATTTGATGTATTTGAGATGGTGGATGGCAAGTAT
    GAAATTAGAGAAAGTAAAGTAATTTCAGGTACCCAATTCATTATTGAATTATTAGGAGTA
    AATGCAGATCAATTCCGACAATTGTTTATTTTGCCTCAAGGTGAATTCAAACGCTTTTTA
    ATATCAAACAGTCGTGAAAAGCAAGGGATATTAAGAACACTGTTTGACAGTGAAAAATTT
    GAAGCTATACGAGAAATATTAAAAGAAGAAGTAAAAAAAGAAAAAGCTCAAATCGAGAAT
    AGATATCAACAAATTGACCTTTTATGGCAAGAAATTGAATCATTTGATGATGACAATATA
    AAAGGCTTATTAGAGGTTGCCACTCAACAGATAGACAAATTGATTGAAAATATACCACTT
    TTACAAGCTAGGTCGAAAGAAATACTAGCATCTGTAAATGAAAGTAAAGAAACTGCTATT
    AAAGAATTTGAAATAATAGAAAAGAAAACATTAGAAAATAATATATTAAAAGATAATATT
    AATCAACTCAACAAAAATAAAATTGATTTCGTTCAATTGAAAGAACAACAACCTGAAATA
    GAGGGAATTGAAGCTAAGTTAAAGTTGTTACAAGATATTACAAACCTATTGAATTATATT
    GAAAATAGAGAAAAAATTGAAACTAAAATTGCTAATAGCAAAAAAGATATTTCTAAAACC
    AATAATAAAATATTGAATCTTGATTGTGATAAGCGAAACATAGACAAAGAGAAAAAAATG
    TTAGAAGAAAATGGAGATTTAATTGAAAGTAAAATCTCTTTTATTGATAAAACTAGAGTA
    TTATTTAACGATATTAATAAGTATCAACAAAGTTATCTCAATATTGAACGCTTGAGAACT
    GAGGGTGAACAATTAGGTGATGAATTAAATGATCTAATTAAAGGTTTAGAAACGGTCGAA
    GATTCAATAGGTAATAACCAAAGTGATTACGAGAAAATTATCGAACTAAATAATACGATA
    ACGAACATAAATAATGAAATTAATATAATTAAAGAAAATGAAAAAGCTAAAGCTGAATTA
    GATAAACTATTAGGTAGTAAGCAAGAGTTAGAGAATCAAATTAATGAAGAAACATCTATA
    TTGAAGAATCTCGAAATAAAATTAGATCGCTACGATAAAACAAAATTGGACTTAAATGAT
    AAAGAAAGCTTTATAAGTGAAATTAAATCTGCTGTAAATATTGGAGATCAATGTCCGATA
    TGTGGTAATGAAATTCAGGATTTAGGGCATCATATTGATTTTGACAGTATTGCTAAACGT
    CAAAATGAAATTAAAGAAATTGAAGCAAATATCCACGCTATAAAATCGAATATTGCTGTG
    CATAATTCTGAAATTAAATTTGTTAATGAAAAAATATCGAATATTAATATTAAAACGCAA
    AGTGATTTTTCACTTGAAGTATTGAATAAGCGTCTGCTAGAAAATGAAAATGCATTGAAT
    AATCAAAGAGATCTTAATAAATTTATAGAACAAATGAAAGAAGAAAAAGATAATCTAACG
    TTGCAAATTCATAATAAACAATTGCGTCTAAATAAAAATGAATCTGAGTTGAAATTATGT
    CGAGATCTCATCACTGAATTTGAAACACTCTCGAAATATAATAATATCACTAATTTTGAG
    GTGGATTATAAGAAGTATGTTCAAGATGTGAATCAGCATCAAGAACTCTCAAAGGAGATT
    GAAGATAAGTTAATGCAATTGTCTCAAAGAAAGTTAATTGAGCAAAATAATCTAAATCAT
    TATGAAAATCAACTAGAAACTTACAATAATGACTTAGAATTGAATGAACAGTCTATTGAA
    ATGGAAATGTCGAGGCTGAATTTAACTGATGACAATGATATAGATGAAATAATAGCCTGG
    AGGGGCGAGCAAGAGGAATTAGAGCAGAAAAGGGATACTTATAAAAAACGTTATCATGAA
    TTTGAAATGGAAATAGCTAGGTTAGAATCATTAACTAAGGATAAAGAGTTATTGGACTCT
    GATAAATTAAAAGATGAATATGAGCTAAAAAAAGGAAAGATGAATACACTGATAGATGAA
    TACTCTGCTGTTCATTATCAATGTCAAAATAATATTAATAAAACACAATCTATAGTTTCG
    CATATTAATTACTTAAATCAAGAATTAAAGGATCAACAAGAAATATTTCAATTGGCTGAA
    ATTGTCAGTGGTAAGAATAACAAAAATCTTACATTGGAAAACTTTGTCTTAATTTACTAT
    TTAGATCAAATTATTGCTCAAGCAAATCTGAGATTAGCAACAATGTCAGATAATCGATAC
    CAACTAATTAGGCGAGAAGCGGTTTCTCATGGTCTTAGTGGCCTAGAAATTGATGTATTT
    GATTTGCATTCAAATAAGTCTAGACATATTAGCTCGTTATCAGGTGGAGAAACTTTCCAA
    TCGTCGCTTGCATTAGCTTTAGGGTTAAGCGAAATTGTACAGCAGCAATCAGGAGGTATT
    TCACTAGAATCAATATTTATTGATGAAGGATTCGGTACATTAGATCAAGAAACGCTTGAA
    ACAGCGTTAGACACTTTATTAAATCTTAAATCAACTGGTAGAATGGTTGGGATTATTTCA
    CATGTGAGCGAATTGAAAAATAGAATACCTTTAGTTTTAGAAGTGAAATCAGATCAATAT
    CAGAGTTCAACAAGATTCAAAAGAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3030

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1382 [new locus tag: SACOL_RS07035 ]
  • symbol: SbcC
  • description: exonuclease SbcC
  • length: 1009
  • theoretical pI: 4.80299
  • theoretical MW: 117317
  • GRAVY: -0.700297

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcC (TIGR00618; HMM-score: 263.3)
    and 7 more
    Genetic information processing DNA metabolism Restriction/modification DNA sulfur modification protein DndD (TIGR03185; HMM-score: 30.7)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 18.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 18.5)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 18.2)
    type III secretion protein HrpB7 (TIGR02559; HMM-score: 8.7)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 8.6)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 8.6)
  • TheSEED  :
    • Exonuclease SbcC
    DNA Metabolism DNA repair DNA repair, bacterial  Exonuclease SbcC
  • PFAM:
    P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 93.3)
    and 7 more
    SbcCD_C; Putative exonuclease SbcCD, C subunit (PF13558; HMM-score: 49.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 29.1)
    AAA_27; AAA domain (PF13514; HMM-score: 25.8)
    SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 16.4)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.3)
    no clan defined DUF3090; Protein of unknown function (DUF3090) (PF11290; HMM-score: 7)
    SRP1_TIP1; Seripauperin and TIP1 family (PF00660; HMM-score: 6.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008049
    • TAT(Tat/SPI): 0.001824
    • LIPO(Sec/SPII): 0.000309
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIFDAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGNTTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFLISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDDNIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNILKDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSKKDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSYLNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINIIKENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEIKSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIKFVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIHNKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 134 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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