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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1678 [new locus tag: SACOL_RS08560 ]
  • pan locus tag?: SAUPAN004224000
  • symbol: SACOL1678
  • pan gene symbol?: —
  • synonym:
  • product: luciferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1678 [new locus tag: SACOL_RS08560 ]
  • symbol: SACOL1678
  • product: luciferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1708374..1709387
  • length: 1014
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCGATTAAATATTCAGCATTAAACCTTGTCCCTATTCGAGAAGGTGAAGATGAACAA
    ACAGCAATTAATGATATGGTTAATCTCGCACAACATTTAGACGAATTATCATATGAAAGA
    TATTGGATTGCTGAACACCATAACGCTCCCAACCTAGTAAGTTCAGCAACTGCTTTATTA
    ATTCAACATACGTTAGAACATACGAAACACATACGTGTAGGTTCTGGAGGCATCATGTTA
    CCTAATCATGCTCCATTAATCGTTGCGGAACAATTTGGCACGATGGCAACATTATTTCCA
    AATCGTGTCGATTTAGGATTAGGACGTGCACCTGGAACAGATATGATGACCGCAAGTGCA
    TTAAGACGAGATCAACATGATGGTGTTTATAAATTTCCAGAAGAGGTTTCATTATTACAA
    CAATATTTCGGCCCTGCTCACCAACAAGCATATGTTCGTGCTTATCCAGCAGTAGGTAAA
    AATGTGCCTTTATACATTCTTGGTTCTTCAACAGATTCTGCACATTTAGCTGCTCGCAAA
    GGGCTTCCATATGTGTTCGCTGGACATTTTGCACCTCAACAAATGAAAGAAGCTATCGAA
    ATTTACAAAACGTTATTTGAACCTTCTGATGTATTAGACGAACCTTATGTTATTGTATGT
    TTAAATACAATCGTTGCTGAAAATGATGACGAAGCACAATATTTAGCTTCATCTATGGCA
    CAAGTAATGGTTAGTATCACTCGTGGCAGAATGCAGCCCGTTCAACCGCCAACACATGAA
    CTACAAAATATATTAACGCCGAGAGAATACGCGATGGCTATGGAAAGACAGAAAATATCA
    TTAATAGGTTCAGAAAATACTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGT
    GAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAATTAGACTCT
    TATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1678 [new locus tag: SACOL_RS08560 ]
  • symbol: SACOL1678
  • description: luciferase
  • length: 337
  • theoretical pI: 5.35846
  • theoretical MW: 37985
  • GRAVY: -0.273591

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity luciferase family oxidoreductase, group 1 (TIGR03558; EC 1.-.-.-; HMM-score: 416.2)
    and 19 more
    probable oxidoreductase, LLM family (TIGR03858; EC 1.-.-.-; HMM-score: 60.2)
    Unknown function Enzymes of unknown specificity putative luciferase-like monooxygenase, FMN-dependent, CE1758 family (TIGR04036; EC 1.-.-.-; HMM-score: 44.1)
    natural product biosynthesis luciferase-like monooxygenase domain (TIGR04020; EC 1.-.-.-; HMM-score: 37.7)
    Unknown function Enzymes of unknown specificity putative FMN-dependent luciferase-like monooxygenase, KPN_01858 family (TIGR04027; EC 1.-.-.-; HMM-score: 35.7)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv2161c family (TIGR03619; EC 1.-.-.-; HMM-score: 33.3)
    Unknown function Enzymes of unknown specificity luciferase-type oxidoreductase, BA3436 family (TIGR03571; EC 1.-.-.-; HMM-score: 32.9)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_3544 family (TIGR03854; HMM-score: 29.3)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_4141 family (TIGR03620; HMM-score: 27.7)
    Metabolism Energy metabolism Methanogenesis 5,10-methylenetetrahydromethanopterin reductase (TIGR03555; EC 1.5.98.2; HMM-score: 24.5)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2249 family (TIGR03857; HMM-score: 22)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, MSMEG_2516 family (TIGR03621; HMM-score: 21)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv3093c family (TIGR03841; EC 1.-.-.-; HMM-score: 19.9)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, MSMEG_4879 family (TIGR03564; EC 1.-.-.-; HMM-score: 17.3)
    Metabolism Energy metabolism Pentose phosphate pathway glucose-6-phosphate dehydrogenase (coenzyme-F420) (TIGR03554; EC 1.1.98.2; HMM-score: 16.9)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv3520c family (TIGR03559; HMM-score: 15.1)
    TAT-translocated F420-dependent dehydrogenase, FGD2 family (TIGR04465; EC 1.-.-.-; HMM-score: 15)
    Unknown function Enzymes of unknown specificity F420-dependent oxidoreductase, CPS_4043 family (TIGR03842; HMM-score: 14.5)
    Unknown function Enzymes of unknown specificity coenzyme F420-dependent oxidoreductase, NP1902A family (TIGR04024; EC 1.14.-.-; HMM-score: 13.3)
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 12.4)
  • TheSEED  :
    • Flavin-utilizing monoxygenase
  • PFAM:
    no clan defined Bac_luciferase; Luciferase-like monooxygenase (PF00296; HMM-score: 124)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003671
    • TAT(Tat/SPI): 0.000101
    • LIPO(Sec/SPII): 0.000305
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIAEHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNRVDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGKNVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVIVCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMERQKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEKNTL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 59 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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