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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1686 [new locus tag: SACOL_RS08600 ]
  • pan locus tag?: SAUPAN004236000
  • symbol: hisS
  • pan gene symbol?: hisS
  • synonym:
  • product: histidyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1686 [new locus tag: SACOL_RS08600 ]
  • symbol: hisS
  • product: histidyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1715050..1716312
  • length: 1263
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTAAAATACCTAGAGGGACGCAGGATATTTTACCTGAAGATTCAAAGAAATGGCGT
    TACATTGAAAATCAATTAGATGAATTAATGACATTTTATAATTATAAAGAAATAAGAACA
    CCAATTTTTGAAAGTACAGATCTTTTTGCAAGAGGTGTTGGTGATTCAACCGATGTCGTA
    CAAAAAGAAATGTATACATTTAAAGATAAAGGCGATAGAAGTATTACATTAAGACCTGAG
    GGAACAGCTGCAGTTGTGCGTTCATATATTGAACATAAAATGCAAGGTAATCCAAACCAA
    CCAATTAAACTTTATTACAATGGACCGATGTTTAGATATGAACGTAAGCAAAAAGGACGC
    TATCGTCAATTTAATCAATTTGGTGTAGAAGCTATTGGTGCTGAAAATCCTAGCGTAGAT
    GCAGAAGTATTAGCTATGGTTATGCATATTTATCAATCATTTGGATTAAAACATTTAAAG
    CTTGTTATTAATAGTGTAGGGGATATGGCGTCTCGAAAAGAATATAACGAAGCGTTAGTG
    AAACACTTTGAACCAGTAATTCATGAATTTTGTTCAGATTGTCAATCACGTTTGCATACA
    AATCCGATGCGAATTTTGGATTGTAAAGTAGACCGTGATAAAGAAGCGATTAAGACTGCA
    CCTAGAATCACTGATTTCTTAAATGAGGAATCTAAGGCATATTATGAACAAGTAAAAGCT
    TATTTAGATGATTTAGGTATTCCATATATTGAAGATCCTAACTTAGTTCGTGGATTGGAT
    TATTATACACATACAGCATTTGAATTAATGATGGATAACCCTAACTATGATGGTGCCATT
    ACAACGCTTTGTGGTGGTGGCCGTTATAATGGTTTATTAGAATTGCTAGATGGTCCAAGT
    GAAACAGGTATTGGTTTTGCGCTAAGTATAGAACGATTATTGCTTGCACTTGAAGAAGAA
    GGTATCGAATTAGATATTGAAGAAAACTTAGATTTATTCATTGTTACAATGGGTGATCAA
    GCAGATCGATATGCTGTGAAGCTATTAAATCATTTGAGACATAATGGTATTAAAGCAGAT
    AAAGACTATTTACAGCGTAAAATTAAAGGACAAATGAAACAAGCAGACCGTTTAGGTGCC
    AAGTTTACAATCGTTATTGGTGATCAAGAATTAGAAAATAATAAAATCGATGTTAAAAAT
    ATGACAACTGGTGAATCTGAAACAATTGAATTAGACGCATTAGTCGAATATTTTAAGAAG
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1686 [new locus tag: SACOL_RS08600 ]
  • symbol: HisS
  • description: histidyl-tRNA synthetase
  • length: 420
  • theoretical pI: 5.01807
  • theoretical MW: 48282.6
  • GRAVY: -0.519048

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.21?  ExPASy
    Histidine--tRNA ligase ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 510.5)
    and 7 more
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 169.6)
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 57.1)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 31.9)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 24.6)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 19.6)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 14)
    Genetic information processing Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 12.6)
  • TheSEED  :
    • Histidyl-tRNA synthetase (EC 6.1.1.21)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Stringent Response, (p)ppGpp metabolism  Histidyl-tRNA synthetase (EC 6.1.1.21)
    and 1 more
    Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  Histidyl-tRNA synthetase (EC 6.1.1.21)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 162.3)
    and 4 more
    tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 72.7)
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 70.2)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 15.8)
    IIaaRS-ABD (CL0458) HGTP_anticodon2; Anticodon binding domain of tRNAs (PF12745; HMM-score: 15.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001648
    • TAT(Tat/SPI): 0.000122
    • LIPO(Sec/SPII): 0.000183
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFESTDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPIKLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLKLVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIKTAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELENNKIDVKNMTTGESETIELDALVEYFKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 656 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Asn) (transcription antitermination) regulon
    T-box(Asn)(RNA)important in Amino acid metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 24.07 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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