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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1781 [new locus tag: SACOL_RS09115 ]
  • pan locus tag?: SAUPAN004393000
  • symbol: SACOL1781
  • pan gene symbol?: isdH
  • synonym:
  • product: cell wall surface anchor family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1781 [new locus tag: SACOL_RS09115 ]
  • symbol: SACOL1781
  • product: cell wall surface anchor family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1821378..1824065
  • length: 2688
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAACAAACATCACCCAAAATTAAGGTCTTTCTATTCTATTAGAAAATCAACTCTAGGC
    GTTGCATCGGTCATTGTCAGTACACTATTTTTAATTACTTCTCAACATCAAGCACAAGCA
    GCAGAAAATACAAATACTTCAGATAAAATCTCGGAAAATCAAAATAATAATGCAACTACA
    ACTCAGCCACCTAAGGATACAAATCAAACACAACCTGCTACGCAACCAGCAAACACTGCG
    AAAAACTATCCTGCAGCGGATGAATCACTTAAAGATGCAATTAAAGATCCTGCATTAGAA
    AATAAAGAACATGATATAGGTCCAAGAGAACAAGTCAATTTCCAGTTATTAGATAAAAAC
    AATGAAACGCAGTACTATCACTTTTTCAGCATCAAAGATCCAGCAGATGTGTATTACACT
    AAAAAGAAAGCAGAAGTTGAATTAGACATCAATACTGCTTCAACATGGAAGAAGTTTGAA
    GTCTATGAAAACAATCAAAAATTGCCAGTGAGACTTGTATCATATAGTCCTGTACCAGAA
    GACCATGCCTATATTCGATTCCCAGTTTCAGATGGCACACAAGAATTGAAAATTGTTTCT
    TCGACTCAAATTGATGATGGAGAAGAAACAAATTATGATTATACTAAATTAGTATTTGCT
    AAACCTATTTATAACGATCCTTCACTTGTAAAATCAGATACAAATGATGCAGTAGTAACG
    AATGATCAATCAAGTTCAGTCGCAAGTAATCAAACAAACACGAATACATCTAATCAAAAT
    ACATCAACGATCAACAATGCTAATAATCAACCGCAGGCAACGACCAATATGAGTCAACCT
    GCACAACCAAAATCGTCAACGAATGCAGATCAAGCGTCAAGCCAACCAGCTCATGAAACA
    AATTCTAATGGTAATACTAACGATAAAACGAATGAGTCAAGTAATCAGTCGGATGTTAAT
    CAACAGTATCCACCAGCAGATGAATCACTACAAGATGCAATTAAAAACCCGGCTATCATC
    GATAAAGAACATACAGCTGATAATTGGCGACCAATTGATTTTCAAATGAAAAATGATAAA
    GGTGAAAGACAGTTCTATCATTATGCTAGTACTGTTGAACCAGCAACTGTCATTTTTACA
    AAAACAGGACCAATAATTGAATTAGGTTTAAAGACAGCTTCAACATGGAAGAAATTTGAA
    GTTTATGAAGGTGACAAAAAGTTACCAGTCGAATTAGTATCATATGATTCTGATAAAGAT
    TATGCCTATATTCGTTTCCCAGTATCTAATGGTACGAGAGAAGTTAAAATTGTGTCATCT
    ATTGAATATGGTGAGAACATCCATGAAGACTATGATTATACGCTAATGGTCTTTGCACAG
    CCTATTACTAATAACCCAGACGACTATGTGGATGAAGAAACATACAATTTACAAAAATTA
    TTAGCTCCGTATCACAAAGCTAAAACGTTAGAAAGACAAGTTTATGAATTAGAAAAATTA
    CAAGAGAAATTGCCAGAAAAATATAAGGCGGAATATAAAAAGAAATTAGATCAAACTAGA
    GTAGAGTTAGCTGATCAAGTTAAATCAGCAGTGACGGAATTTGAAAATGTTACACCTACA
    AATGATCAATTAACAGATTTACAAGAAGCGCATTTTGTTGTTTTTGAAAGTGAAGAAAAT
    AGTGAGTCAGTTATGGACGGCTTTGTTGAACATCCATTCTATACAGCAACTTTAAATGGT
    CAAAAATATGTAGTGATGAAAACAAAGGATGACAGTTACTGGAAAGATTTAATTGTAGAA
    GGTAAACGTGTCACTACTGTTTCTAAAGATCCTAAAAATAATTCTAGAACGCTGATTTTC
    CCATATATACCTGACAAAGCAGTTTACAATGCGATTGTTAAAGTCGTTGTGGCAAACATT
    GGTTATGAAGGTCAATATCATGTCAGAATTATAAATCAGGATATCAATACAAAAGATGAT
    GATACATCACAAAATAACACGAGTGAACCGCTAAATGTACAAACAGGACAAGAAGGTAAG
    GTTGCTGATACAGATGTAGCTGAAAATAGCAGCACTGCAACAAATCCTAAAGATGCGTCT
    GATAAAGCAGATGTGATAGAACCAGAGTCTGACGTGGTTAAAGATGCTGATAATAATATT
    GATAAAGATGTGCAACATGATGTTGATCATTTATCCGATATGTCGGATAATAATCACTTC
    GATAAATATGATTTAAAAGAAATGGATACTCAAATTGCCAAAGATACTGATAGAAATGTG
    GATAAAGATGCCGATAATAGCGTTGGTATGTCATCTAATGTCGATACTGATAAAGACTCT
    AATAAAAATAAAGACAAAGTCATACAGCTGAATCATATTGCCGATAAAAATAATCATACT
    GGAAAAGCAGCAAAGCTTGACGTAGTGAAACAAAATTATAATAATACAGACAAAGTTACT
    GACAAAAAAACAACTGAACATCTGCCGAGTGATATTCATAAAACTGTAGATAAAACAGTG
    AAAACAAAAGAAAAAGCCGGCACACCATCGAAAGAAAACAAACTTAGTCAATCTAAAATG
    CTACCAAAAACTGGAGAAACAACTTCAAGCCAATCATGGTGGGGCTTATATGCGTTATTA
    GGTATGTTAGCTTTATTCATTCCTAAATTCAGAAAAGAATCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2688

⊟Protein[edit | edit source]

Protein Data Bank: 4IJ2
Protein Data Bank: 4XS0

⊟General[edit | edit source]

  • locus tag: SACOL1781 [new locus tag: SACOL_RS09115 ]
  • symbol: SACOL1781
  • description: cell wall surface anchor family protein
  • length: 895
  • theoretical pI: 4.84648
  • theoretical MW: 100934
  • GRAVY: -0.943911

⊟Function[edit | edit source]

  • TIGRFAM:
    haptoglobin-binding heme uptake protein HarA (TIGR03658; HMM-score: 2116.9)
    and 4 more
    heme uptake protein IsdB (TIGR03657; HMM-score: 667)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 38)
    heme uptake protein IsdC (TIGR03656; HMM-score: 26.4)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 23.8)
  • TheSEED  :
    • Cell surface receptor IsdH for hemoglobin-haptoglobin complexes
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Cell surface receptor IsdH for hemoglobin-haptoglobin complexes
  • PFAM:
    no clan defined NEAT; Iron Transport-associated domain (PF05031; HMM-score: 105.6)
    and 3 more
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 26.7)
    DUF2283; Protein of unknown function (DUF2283) (PF10049; HMM-score: 20.8)
    DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 7.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide SP(Sec/SPI) length 40 aa
    • SP(Sec/SPI): 0.990402
    • TAT(Tat/SPI): 0.008094
    • LIPO(Sec/SPII): 0.000828
    • Cleavage Site: CS pos: 40-41. AQA-AE. Pr: 0.9895
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENTNTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENKEHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFEVYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLVFAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTNMSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAIKNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKTASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHEDYDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKYKAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMDGFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVADTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHFDKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNNHTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLSQSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Agnieszka Dryla, Dieter Gelbmann, Alexander von Gabain, Eszter Nagy
Identification of a novel iron regulated staphylococcal surface protein with haptoglobin-haemoglobin binding activity.
Mol Microbiol: 2003, 49(1);37-53
[PubMed:12823809] [WorldCat.org] [DOI] (P p)Agnieszka Dryla, Bernd Hoffmann, Dieter Gelbmann, Carmen Giefing, Markus Hanner, Andreas Meinke, Annaliesa S Anderson, Walter Koppensteiner, Robert Konrat, Alexander von Gabain, Eszter Nagy
High-affinity binding of the staphylococcal HarA protein to haptoglobin and hemoglobin involves a domain with an antiparallel eight-stranded beta-barrel fold.
J Bacteriol: 2007, 189(1);254-64
[PubMed:17041047] [WorldCat.org] [DOI] (P p)

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