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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • pan locus tag?: SAUPAN004914000
  • symbol: SACOL1951
  • pan gene symbol?: murT
  • synonym:
  • product: Mur ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • symbol: SACOL1951
  • product: Mur ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 2011993..2013306
  • length: 1314
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGACAGTGGACGGCAATCCATCTAGCGAAATTGGCGCGTAAAGCAAGTAGAGCAGTA
    GGTAAAAGAGGAACAGATTTACCTGGACAAATCGCTAGAAAAGTGGATACAGATATATTA
    AGAAAATTAGCAGAGCAAGTTGATGATATTGTATTTATCAGTGGAACAAATGGTAAAACA
    ACGACTTCAAACTTAATTGGACATACTTTAAAAGCAAATAATATTCAAATTATACACAAT
    AATGAAGGTGCTAATATGGCTGCAGGTATAACTTCTGCATTCATCATGCAATCAACACCT
    AAGACTAAAATTGCGGTAATCGAAATTGATGAAGGTTCGATTCCACGTGTGTTAAAAGAA
    GTTACACCTTCAATGATGGTATTTACTAATTTCTTTAGAGATCAAATGGATCGCTTCGGT
    GAAATTGATATTATGGTTAATAACATTGCAGAGACAATTAGTAATAAAGGCATCAAATTA
    TTGCTAAATGCTGATGATCCATTTGTGAGTCGTTTGAAAATCGCAAGTGATACGATTGTG
    TACTATGGTATGAAAGCACATGCCCATGAATTTGAACAAAGTACGATGAATGAAAGTAGA
    TATTGTCCAAACTGTGGTCGCTTATTGCAATACGATTATATTCATTATAATCAAATTGGT
    CATTATCACTGTCAGTGTGGTTTCAAACGAGAGCAAGCAAAATATGAAATATCAAGTTTT
    GATGTGGCACCGTTTTTATATTTAAATATCAATGATGAAAAATATGATATGAAAATTGCA
    GGTGACTTTAACGCTTATAACGCGTTAGCAGCATATACTGTTTTAAGAGAGCTAGGGTTA
    AATGAACAAACAATTAAAAATGGCTTTGAAACGTATACATCAGACAATGGTCGTATGCAG
    TACTTTAAAAAAGAACGAAAAGAAGCGATGATCAATTTAGCTAAAAATCCTGCAGGAATG
    AATGCAAGTTTATCAGTTGGTGAACAATTAGAAGGCGAAAAAGTGTATGTTATTTCGCTA
    AATGATAACGCTGCAGATGGTCGAGATACTTCATGGATTTATGATGCAGATTTTGAAAAA
    TTATCTAAGCAACAAATTGAAGCTATCATCGTGACAGGTACACGAGCAGAAGAACTTCAA
    TTGCGATTGAAGTTAGCAGAGGTTGAAGTACCAATTATAGTTGAGCGTGATATTTATAAA
    GCAACGGCAAAGACTATGGATTATAAAGGTTTCACAGTTGCAATACCAAACTATACATCA
    TTAGCGCCTATGCTTGAACAATTAAACCGTTCGTTTGAAGGAGGTCAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1951 [new locus tag: SACOL_RS10205 ]
  • symbol: SACOL1951
  • description: Mur ligase
  • length: 437
  • theoretical pI: 6.52509
  • theoretical MW: 49205.7
  • GRAVY: -0.349428

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 64.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 61.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 58.3)
    and 6 more
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 46.7)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 45.8)
    Cell structure Cell envelope Other poly-gamma-glutamate synthase PgsB (TIGR04012; EC 6.3.2.-; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 22)
    HprK-related kinase A (TIGR04352; HMM-score: 16.9)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 14.1)
  • TheSEED  :
    • proposed amino acid ligase found clustered with an amidotransferase
  • PFAM:
    no clan defined DUF1727; Domain of unknown function (DUF1727) (PF08353; HMM-score: 101.6)
    and 6 more
    Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 68.6)
    Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 16.6)
    RING (CL0229) zf-C3H2C3; Zinc-finger (PF17122; HMM-score: 13.5)
    Zn_Beta_Ribbon (CL0167) OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 12.8)
    TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 12.7)
    zf-RING_7; C4-type zinc ribbon domain (PF02591; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025235
    • TAT(Tat/SPI): 0.001676
    • LIPO(Sec/SPII): 0.003894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLAEQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKTKIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKLLLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQIGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLRELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEKVYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPIIVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 96 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 22.74 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Daniela Münch, Terry Roemer, Sang Ho Lee, Marianne Engeser, Hans Georg Sahl, Tanja Schneider
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
PLoS Pathog: 2012, 8(1);e1002509
[PubMed:22291598] [WorldCat.org] [DOI] (I p)

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