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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2085 [new locus tag: SACOL_RS10910 ]
  • pan locus tag?: SAUPAN005375000
  • symbol: thiD2
  • pan gene symbol?: thiD
  • synonym:
  • product: phosphomethylpyrimidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2085 [new locus tag: SACOL_RS10910 ]
  • symbol: thiD2
  • product: phosphomethylpyrimidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2150000..2150830
  • length: 831
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGATTAAACCTAAAATAGCATTAACCATTGCAGGTACTGATCCAACAGGTGGTGCCGGC
    GTAATGGCTGATTTAAAATCATTTCATTCATGTGGTGTATATGGTATGGGCGTCGTTACA
    AGTATTGTTGCTCAAAATACATTGGGCGTACAACATATTCATAATTTAAATCATCAATGG
    GTAGATGAACAACTTGATAGTGTCTTCAATGATACCTTACCTCATGCTATTAAAACGGGG
    ATGATTGCTACAGCAGATACTATGGAAACGATTCGTCATTATTTAATGCAACATGAATCT
    ATTCCATATGTAATTGATCCTGTTATGTTGGCGAAAAGCGGTGATTCATTAATGGATAAT
    GACACAAAGCAAAACTTGCAGCATACGTTATTGCCATTAGCTGACGTAGTAACACCGAAT
    TTACCAGAAGCTGAAGAAATAACGGGACTAACCATTGATAGTGAAGAAAAAATTATGCAG
    GCTGGCCGCATCTTTATTAATGAGATTGGTAGTAAAGGTGTCATCATTAAAGGCGGTCAT
    TCAAATGATACTGATATAGCAAAAGATTATTTATTTACTAACGAAGGTGTTCAAACATTT
    GAAAATGAACGATTTAAAACAAAACATACGCATGGAACAGGGTGTACATTTTCAGCAGTT
    ATAACGGCAGAACTTGCAAAAGGTAGACCATTATTTGAGGCTGTACACAAGGCTAAAAAG
    TTTATTTCAATGAGTATACAATATACGCCTGAAATCGGCCGTGGTAGAGGTCCAGTGAAT
    CATTTTGCATATTTAAAGAAAGAGGGATTAGACGATGAATTATCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2085 [new locus tag: SACOL_RS10910 ]
  • symbol: ThiD2
  • description: phosphomethylpyrimidine kinase
  • length: 276
  • theoretical pI: 6.18122
  • theoretical MW: 30228.3
  • GRAVY: -0.223551

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.4.7?  ExPASy
    Phosphooxymethylpyrimidine kinase ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 312)
    and 5 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 57.6)
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 42)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 24.4)
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 23)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 19.8)
  • TheSEED  :
    • Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)
  • PFAM:
    Ribokinase (CL0118) Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 311.1)
    and 1 more
    PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 47.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.089094
    • TAT(Tat/SPI): 0.00202
    • LIPO(Sec/SPII): 0.00546
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVAQNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIPYVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQAGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFSAVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: THI-box (transcription termination) regulon
    THI-box(RNA)important in Thiamine biosynthesis; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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