From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2098 [new locus tag: SACOL_RS10980 ]
  • pan locus tag?: SAUPAN005397000
  • symbol: atpH
  • pan gene symbol?: atpH
  • synonym:
  • product: F0F1 ATP synthase subunit delta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2098 [new locus tag: SACOL_RS10980 ]
  • symbol: atpH
  • product: F0F1 ATP synthase subunit delta
  • replicon: chromosome
  • strand: -
  • coordinates: 2161000..2161539
  • length: 540
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGTAAAAGTAGCTAACAAGTATGCTAAAGCATTATTTGACGTGTCATTAGATACAAAT
    AATTTAGAGACTATTAATGAAGAATTAACAGTTATAAATGAAGCAGTAAAAGATAAAATT
    GAGCAATTGAGAATGGTTGACAGTAATCCAACTCAAACAGCAGAACAACGTCGTGAGTTG
    ATTAATGGTGTATTTACTGACATTAATCCATACATTAAAAATATGATGTACGTGTTAGCA
    GATAATAGACATATCTCATTAATAGCTGACGTATTCAAGGCGTTCCAAAGCTTATATAAC
    GGACACTACAATCAAGATTTTGCAACAATTGAGTCAACATATGAATTGAGTCAAGAAGAG
    TTAGATAAGATTGTCAAACTAGTAACTCAACAAACGAAGTTATCTAAAGTTATTGTAGAT
    ACAAAAATTAATCCAGATTTAATTGGTGGATTTAGAGTTAAAGTCGGCACAACTGTATTA
    GATGGTAGTGTTAGAAATGATCTTGTCCAATTACAAAGAAAATTTAGAAGAGTTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2098 [new locus tag: SACOL_RS10980 ]
  • symbol: AtpH
  • description: F0F1 ATP synthase subunit delta
  • length: 179
  • theoretical pI: 6.55013
  • theoretical MW: 20498.3
  • GRAVY: -0.319553

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.3.14?  ExPASy
    H+-transporting two-sector ATPase ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
  • TIGRFAM:
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, delta subunit (TIGR01145; EC 3.6.3.14; HMM-score: 118.5)
    and 1 more
    alternate F1F0 ATPase, F0 subunit B (TIGR03321; EC 3.6.3.-; HMM-score: 23.9)
  • TheSEED  :
    • ATP synthase delta chain (EC 3.6.3.14)
    Respiration ATP synthases F0F1-type ATP synthase  ATP synthase delta chain (EC 3.6.3.14)
  • PFAM:
    ATP_synthase (CL0255) OSCP; ATP synthase delta (OSCP) subunit (PF00213; HMM-score: 147.9)
    and 6 more
    GT-A (CL0110) Glyco_transf_15; Glycolipid 2-alpha-mannosyltransferase (PF01793; HMM-score: 19.6)
    PH (CL0266) SPT16; FACT complex subunit (SPT16/CDC68) (PF08644; HMM-score: 16)
    no clan defined KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 14.2)
    Tubulin_C (CL0442) FtsZ_C; FtsZ family, C-terminal domain (PF12327; HMM-score: 12.7)
    HTH (CL0123) Sigma54_CBD; Sigma-54 factor, core binding domain (PF04963; HMM-score: 12.6)
    no clan defined Frankia_peptide; Ribosomally synthesized peptide prototyped by Frankia Franean1_4349. (PF14407; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002591
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.000209
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLRMVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGHYNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVLDGSVRNDLVQLQRKFRRVN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 636 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 25.45 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2098&oldid=90663"