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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2340 [new locus tag: SACOL_RS12290 ]
  • pan locus tag?: SAUPAN005843000
  • symbol: gltS
  • pan gene symbol?: gltS
  • synonym:
  • product: sodium:glutamate symporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2340 [new locus tag: SACOL_RS12290 ]
  • symbol: gltS
  • product: sodium:glutamate symporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2399779..2400987
  • length: 1209
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGATAGAACTTAATGCAATTACAACATTATGTTTAGCTTGTATCCTTTATTTACTTGGT
    AAGGCTATCGTTAATCACGTTAATTTTTTAAAACGTATTTGTATACCAGCACCAGTGATT
    GGCGGCTTAATCTTTGCTATTTTAGTTGCGGCTTTGGATTCATTTGGCATGGTTAAGATT
    AAATTAGATGCTTCATTCATTCAAGATTTCTTCATGTTAGCATTCTTTACGACAATCGGT
    CTTGGTGCATCATTGAAATTATTTAAATTAGGTGGCAAAGTCTTGCTATTATACTTTATG
    TTTTGTGCTATCATTTCAGTCATTCAAAACATAGTTGGTGTATCACTAGCAAAAGTATTA
    AATATTAAACCTTTGTTAGGATTAACAGCAGGTTCCATGTCTATGGAAGGCGGTCATGGT
    AATGCTGCTGCTTATGGTAAGACAATTCAAGATTTAGGTATTGATTCGGCACTGACAGCG
    GCTCTTGCAGCTGCAACTTTAGGTCTTGTATTTGGAGGGCTTATCGGTGGTCCAGTTGTA
    AAATTTTTAATCAAACGTTATAACTTAAAGCCTCAACATAGTGACGATACATTTAAAGAT
    TATAGCCAAGTAGCATATAACGAACATTTACATAGTAAATTTAATGCCACTGAAGTATTC
    TTCATTCAATTTACAATCGTTGTATTCTGTATGGCAGTTGGAAGTTATTTCAGTCATTTG
    TTTACAGCTCAAACAGGGATTAATGTTCCAATTTACGTTGGCTCATTATTTGTAGCTGTT
    ATTGTCCGAAATATCTCTGAAAGTTTTAATTTTAATATTGTAGATTTAAAAATTACTAAT
    CAAATTGGCGATGTCGCATTAGGTATTTTCTTATCTCTTGCGCTAATGAGCATTCAATTA
    ATCGAAATTTATAAACTTGCTATACCTCTTATTATTATCGTTTTAGTTCAAGTTGTCGTT
    ATGATTTTATTTGCTGTTTTAATTTTATTTAGAGGTTTAGGAAAAGATTATGATGCTGCA
    GTAATGGTAGGTGGTTTTATCGGTCATGGGCTTGGTGCAACGCCAAATGCCATGGCAAAT
    TTAGATGTTATTACTAAAAAATATGGAAACTCACCTAAAGCATATTTAGTTGTACCTATT
    GTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATACAATGG
    TTTAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1209

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2340 [new locus tag: SACOL_RS12290 ]
  • symbol: GltS
  • description: sodium:glutamate symporter
  • length: 402
  • theoretical pI: 8.86246
  • theoretical MW: 43263.7
  • GRAVY: 1.01219

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/glutamate symporter (TIGR00210; HMM-score: 358)
  • TheSEED  :
    • Sodium/glutamate symporter
  • PFAM:
    CPA_AT (CL0064) Glt_symporter; Sodium/glutamate symporter (PF03616; HMM-score: 505.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013371
    • TAT(Tat/SPI): 0.000518
    • LIPO(Sec/SPII): 0.017277
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTAALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQWFS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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