From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2392 [new locus tag: SACOL_RS12550 ]
  • pan locus tag?: SAUPAN005925000
  • symbol: narI
  • pan gene symbol?: narI
  • synonym:
  • product: respiratory nitrate reductase subunit gamma

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2392 [new locus tag: SACOL_RS12550 ]
  • symbol: narI
  • product: respiratory nitrate reductase subunit gamma
  • replicon: chromosome
  • strand: -
  • coordinates: 2450438..2451115
  • length: 678
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTTAATCAGTTTTTATGGGTTATATTTCCGTATTTATGTTTAGTTGTCTTTGTCGCA
    GGTCATATTGCACGTTATCGTTATGATAAATTTTCTTGGACAGCTAAATCTAGTGAATTA
    ATAGAAAGAAAAAGGTTGATGTGGGGAAGTTTATTATTTCACTTAGGTATTATTCCAGTG
    TTTTTTGGACACGTAGTTGGATTGTTAATACCGAAACCATGGATGGATGCAGTGGGTGTG
    TCTGAGCATTTATATCATATAGGCGCTGTTTATATTGGTAGCATCTTTGGTATAATAACT
    TTGATAGGGATGTTTTTATTAACAGCGAGACGTGTTACTACGAAAAGTATTCGAAGATTA
    AGTTCAGCGTCGGATATATTTGTAAATTTCTTATTATTACTTATCGTATTTATGGGATGT
    TACGCAACATTAGTAACAAATATACAACATCCAGACTTTAACTACAGAACTTCATTATCA
    ATTTGGTTTAGACAATTATTTATGTTAAAACCAGATGCGTCATTGATGAGTGGTGTGCCG
    ATTGCATTTAAAATGCATATATTGTTAGGTTTCACTATTATGGCTTGTTGGCCATTTACA
    CGTTTGGTGCATGTTTGGAGTGTACCTTTGACATATATAAATCGCAGATATATTATTTAC
    CGTAAGAATAAAGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2392 [new locus tag: SACOL_RS12550 ]
  • symbol: NarI
  • description: respiratory nitrate reductase subunit gamma
  • length: 225
  • theoretical pI: 10.5772
  • theoretical MW: 26145.3
  • GRAVY: 0.651111

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.99.4?  ExPASy
    Nitrate reductase Nitrite + acceptor = nitrate + reduced acceptor
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic respiratory nitrate reductase, gamma subunit (TIGR00351; EC 1.7.99.4; HMM-score: 238.5)
  • TheSEED  :
    • Respiratory nitrate reductase (quinol), gamma subunit (EC 1.7.5.1)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrate and nitrite ammonification  Respiratory nitrate reductase gamma chain (EC 1.7.99.4)
  • PFAM:
    no clan defined Nitrate_red_gam; Nitrate reductase gamma subunit (PF02665; HMM-score: 293.6)
    and 1 more
    UPF0114; Uncharacterized protein family, UPF0114 (PF03350; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013116
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.080568
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERKRLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLIGMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLSIWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYIIYRKNKV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NreC* (activation) regulon
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2392&oldid=96011"