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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2416 [new locus tag: SACOL_RS12680 ]
  • pan locus tag?: SAUPAN005953000
  • symbol: SACOL2416
  • pan gene symbol?: —
  • synonym:
  • product: cation efflux family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2416 [new locus tag: SACOL_RS12680 ]
  • symbol: SACOL2416
  • product: cation efflux family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2475100..2475966
  • length: 867
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCTCATAATGAAAATCTTAAATTGGCGCAACGCGGTGCTTATTTGAGTTTAATTGTA
    TATATAATCTTATCTATTGTTAAGTATGTCACGGGTTTTGTCTTTAACTCTGCAGCTGTA
    AGAGCCGATGCATTAAACAATATGACAGATATTATCGTTTCTTTAGCTGTTATTATTGGA
    TTGAAAATTTCAATTAAACCTGCCGATCGAAATCATCCTTATGGCCATTTGAAGTCTGAA
    AATATTTCTTCATTATTGGTGTCATTTGTCATTATGTTTGTAGGTATCCAAGTAGTTATT
    CAAAATGCACCTCGTTTGTTCAAAGAAGATGACGTTGTACCTAACGCAATAACAATTATC
    GTCAGCTTAATCAGTGGTCTTGTAATGTTGATTGTATTTGCAGTCAATCAAAGACTAGCG
    AAAAGAACGAAAAGTAGTTCTTTAAATTCGGCTGCCAAAGATAATTTATCGGATAGTTTA
    GTCAGCATTGGAACAGCGATAGGATTAATTTTTACGCAAATCGGTTTCCCAATTGTCGAT
    ATTATTTTAGCAACATTACTAGGCTTACTAATCGTTTATACTGGTTTTGGTATTTTTAAA
    GAAGCAATTTTTATGTTGAGTGATGGTTTCAATGAAACTGAATTAGAAGCGTATCGCAAT
    GATATTTTAGAAGTTGATGAAGTACAAGAAGTTAAAAGTATTAAAGGGCGTTACCATGGA
    AGTAGTGTGTTTATTGATGTCACAATTGTTGTAGATGCTAACCTTTCCTTAGTAGAAGCG
    CATCAAATTTGTGACAATGTCGAACATCATTTGCACAAAAAAGGTATTTCCTCAGTATAC
    GTTCACCCAGAACCAGACCATCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    867

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2416 [new locus tag: SACOL_RS12680 ]
  • symbol: SACOL2416
  • description: cation efflux family protein
  • length: 288
  • theoretical pI: 6.34453
  • theoretical MW: 31581.6
  • GRAVY: 0.489583

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation diffusion facilitator family transporter (TIGR01297; HMM-score: 210.8)
  • TheSEED  :
    • Manganese efflux system protein MneS (= MntE)
    Potassium metabolism Potassium metabolism - no subcategory Glutathione-regulated potassium-efflux system and associated functions  Cobalt-zinc-cadmium resistance protein
    and 1 more
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Cobalt-zinc-cadmium resistance  Cobalt-zinc-cadmium resistance protein
  • PFAM:
    no clan defined Cation_efflux; Cation efflux family (PF01545; HMM-score: 155.3)
    and 7 more
    ZT_dimer; Dimerisation domain of Zinc Transporter (PF16916; HMM-score: 65.9)
    EI24; Etoposide-induced protein 2.4 (EI24) (PF07264; HMM-score: 14.6)
    LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.7)
    Transporter (CL0375) Claudin_2; PMP-22/EMP/MP20/Claudin tight junction (PF13903; HMM-score: 9.5)
    no clan defined DUF4396; Domain of unknown function (DUF4396) (PF14342; HMM-score: 7.7)
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 7.2)
    no clan defined DUF5467; Family of unknown function (DUF5467) (PF17555; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.249376
    • TAT(Tat/SPI): 0.01015
    • LIPO(Sec/SPII): 0.002296
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADALNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQNAPRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSLVSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAYRNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGISSVYVHPEPDHL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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