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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2549 [new locus tag: SACOL_RS13345 ]
  • pan locus tag?: SAUPAN006189000
  • symbol: SACOL2549
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2549 [new locus tag: SACOL_RS13345 ]
  • symbol: SACOL2549
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2606694..2607662
  • length: 969
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCGTAAAAAATGGTCTACACTTGCGTTTGGATTTTTAGTTGCAGCATACGCACATATT
    AGAATTAAAGAAAAACGCAGTGTGAAAAGTTATATGTTAGAACAAGGTATACGATTATCT
    AGAGCTAAGCGTCGTTTTATGTATAAAGAAGAAGCGATGAAAGCATTAGAAAAAATGGCG
    CCACAGACAGCAGGCGAATATGAGGGAACCAATTATCAGTTTAAGATGCCAGTAAAAGTG
    GATAAGCACTTCGGTTCAACCGTTTATACCGTTAACGATAAACAAGATAAGCATCAACGC
    GTTGTATTATATGCACATGGAGGCGCATGGTTCCAAGACCCACTCAAAATTCATTTCGAA
    TTTATTGATGAACTTGCAGAAACACTCAATGCTAAAGTCATCATGCCAGTATATCCGAAG
    ATTCCGCATCAAGATTATCAAGCGACGTATGTGCTTTTTGAAAAGTTGTACCATGATTTA
    TTGAATCAAGTAGCAGATTCTAAACAAATCGTTGTAATGGGTGACTCTGCGGGCGGTCAA
    ATTGCTTTATCATTTGCTCAATTGTTAAAAGAAAAACATATTGTGCAACCAGGACATATT
    GTATTAATTTCACCAGTTTTAGATGCAACGATGCAGCATCCTGAAATTCCTGACTACTTA
    AAGAAAGACCCAATGGTAGGTGTGGATGGCAGTGTGTTCTTAGCTGAACAATGGGCAGGG
    GACACACCTTTAGATAACTACAAAGTATCACCAATTAATGGTGACTTAGATGGTTTAGGT
    CGTATTACATTAACTGTTGGTACAAAAGAAGTACTGTACCCAGATGCTTTGAACTTATCG
    CAATTGTTGAGTGCGAAAGGTATCGAACATGACTTTATACCTGGATATTACCAATTCCAT
    ATTTATCCAGTATTTCCGATACCAGAGCGACGACGCTTTTTATACCAAGTGAAAAATATT
    ATTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2549 [new locus tag: SACOL_RS13345 ]
  • symbol: SACOL2549
  • description: hypothetical protein
  • length: 322
  • theoretical pI: 9.11945
  • theoretical MW: 36847.4
  • GRAVY: -0.26087

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity probable F420-dependent oxidoreductase, Rv1855c family (TIGR03560; HMM-score: 11.9)
  • TheSEED  :
    • Esterase/lipase (EC 3.1.1.-)
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 104.8)
    and 9 more
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 55.6)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 19.7)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 18.6)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 17.1)
    Esterase; Putative esterase (PF00756; HMM-score: 15.2)
    AXE1; Acetyl xylan esterase (AXE1) (PF05448; HMM-score: 14.1)
    no clan defined UPF0240; Uncharacterised protein family (UPF0240) (PF06784; HMM-score: 13.4)
    AB_hydrolase (CL0028) BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 13.1)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.5
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.224571
    • TAT(Tat/SPI): 0.004058
    • LIPO(Sec/SPII): 0.162038
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKRRFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVVLYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDLLNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPDYLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDALNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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