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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2728 [new locus tag: SACOL_RS14265 ]
  • pan locus tag?: SAUPAN006470000
  • symbol: SACOL2728
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2728 [new locus tag: SACOL_RS14265 ]
  • symbol: SACOL2728
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2799596..2800093
  • length: 498
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGATTATTAACCTTGAAACACAACAAATACATTTTGATTTAGAACATGAAATTCCCTAT
    TTTCAAGCACCTGAGAAAAATAGGATACGCTTAGATATTGATGTTCTCAATAAAAAGCAA
    ATTTCTAATATTATTAATGTTATATTTAATAACCAATCAAAGACGAAATGTACATTTTTG
    TCTGAATACTTATATCCTGTTAAATTTAGAGAAAAAACAAGAATTGGTCGTTTTTTTAAT
    ATTACTAACTGGTACGAAGAAATCCATTCTACCGATGAGAAGTATGTTATTGCAACTATT
    AAAAATATAAAAAACAAAGACTTAATTGATTATTCTTTATATATTAAAAAAGGTTTAGTG
    GAGCCATTTATTGTATTCTATAATCAAGAAAATATATTCTACATTAGCAATGCTGTTATA
    GATATCATCAGTGTATCCAAAGATACTCTTGATTATATAAAGTCAGAATTCAATGATCAC
    TGTGCTTCGCCATACTGA
    60
    120
    180
    240
    300
    360
    420
    480
    498

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2728 [new locus tag: SACOL_RS14265 ]
  • symbol: SACOL2728
  • description: hypothetical protein
  • length: 165
  • theoretical pI: 6.51676
  • theoretical MW: 19705.4
  • GRAVY: -0.320606

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • FIG01108146: hypothetical protein
  • PFAM:

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003863
    • TAT(Tat/SPI): 0.000288
    • LIPO(Sec/SPII): 0.001497
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNIINVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKNIKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDHCASPY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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