COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00005 [old locus tag: SAA0001 ]
• pan locus tag^?:
• symbol: SACOL_RS00005
• pan gene symbol^?: —
• synonym:
• product: replication initiation protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00005 [old locus tag: SAA0001 ]
• symbol: SACOL_RS00005
• product: replication initiation protein
• replicon: pT181
• strand: +
• coordinates: 32..976
• length: 945
• essential: unknown

⊟Accession numbers

• Location: NC_006629 (32..976) NCBI
• BioCyc: SACOL_RS00005 BioCyc
• MicrobesOnline: see SAA0001

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTATAAAAACAATCATGCAAATCATTCAAATCATTTGGAAAATCACGATTTAGACAAT
  TTTTCTAAAACCGGCTACTCTAATAGCCGGTTGGACGCACATACTGTGTGCATATCTGAT
  CCAAAATTAAGTTTTGATGCAATGACGATCGTTGGAAATCTCAACCGAGACAACGCTCAA
  GCCCTTTCTAAATTTATGAGTGTAGAGCCCCAAATAAGACTTTGGGATATTCTTCAAACA
  AAGTTTAAAGCTAAAGCACTTCAAGAAAAAGTTTATATTGAATATGACAAAGTGAAAGCA
  GATAGTTGGGATAGACGTAATATGCGTATTGAATTTAATCCAAACAAACTTACACGAGAT
  GAAATGATTTGGTTAAAACAAAATATAATAAGCTACATGGAAGATGACGGTTTTACAAGA
  TTAGATTTAGCCTTTGATTTTGAAGATGATTTGAGTGACTACTATGCAATGTCTGATAAA
  GCAGTTAAGAAAACTATTTTTTATGGTCGTAATGGTAAGCCAGAAACAAAATATTTTGGC
  GTGAGAGATAGTAATAGATTTATTAGAATTTATAATAAAAAGCAAGAACGTAAAGATAAT
  GCAGATGCTGAAGTTATGTCTGAACATTTATGGCGTGTAGAAATCGAACTTAAAAGAGAT
  ATGGTGGATTACTGGAATGATTGCTTTAGTGATTTACATATCTTGCAACCAGATTGGAAA
  ACTATCCAACGCACTGCGGATAGAGCAATAGTTTTTATGTTATTGAGTGATGAAGAAGAA
  TGGGGAAAGCTTCACAGAAATTCTAGAACAAAATATAAGAATTTGATAAAAGAAATTTCG
  CCAGTCGATTTAACGGACTTAATGAAATCGACTTTAAAAGCGAACGAAAAACAATTGCAA
  AAACAAATCGATTTTTGGCAACATGAATTTAAATTTTGGAAATAG
  60
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  945
  

⊟Protein

⊟General

• locus tag: SACOL_RS00005 [old locus tag: SAA0001 ]
• symbol: SACOL_RS00005
• description: replication initiation protein
• length: 314
• theoretical pI: 7.32108
• theoretical MW: 37642.4
• GRAVY: -0.848089

⊟Function

• TIGRFAM:
• TheSEED: see SAA0001
• PFAM:
  TBP-like (CL0407) Rep_trans; Replication initiation factor (PF02486; HMM-score: 40.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011766
  • TAT(Tat/SPI): 0.001372
  • LIPO(Sec/SPII): 0.001683
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446199799 NCBI
• RefSeq: WP_000277654 NCBI
• UniProt: see SAA0001

⊟Protein sequence

• MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSFDAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWDRRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISPVDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK

⊟Experimental data

• experimentally validated: see SAA0001
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications