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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00165 [old locus tag: SACOL0028 ]
  • pan locus tag?: SAUPAN000054000
  • symbol: SACOL_RS00165
  • pan gene symbol?: tnp
  • synonym:
  • product: IS6 family transposase IS431mec

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00165 [old locus tag: SACOL0028 ]
  • symbol: SACOL_RS00165
  • product: IS6 family transposase IS431mec
  • replicon: chromosome
  • strand: -
  • coordinates: 36353..37027
  • length: 675
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (36353..37027) NCBI
  • BioCyc: SACOL_RS00165 BioCyc
  • MicrobesOnline: see SACOL0028

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAC
    TATCTAAGATATACATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTA
    AACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTGTATCAA
    ATTTGGAAGAAAAAGCATAAAAAAGCTTATTACAAATGGCGTATTGATGAGACGTACATC
    AAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTGATGCAGAGGGACATACATTA
    GATATTTGGTTGCGTAAGCAACGAGATAATCATTCAGCATATGCGTTTATCAAACGTCTC
    ATTAAACAATTTGGTAAACCTCAAAAGGTAATTACAGATCAGGCACCTTCAACGAAGGTA
    GCAATGGCTAAAGTAATTAAAGCTTTTAAACTTAAACCTGACTGTCATTGTACATCGAAA
    TATCTGAATAACCTCATTGAGCAAGATCACCGTCATATTAAAGTAAGAAAGACAAGGTAT
    CAAAGTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATAT
    AAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATC
    ATGCTAGCAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS00165 [old locus tag: SACOL0028 ]
  • symbol: SACOL_RS00165
  • description: IS6 family transposase IS431mec
  • length: 224
  • theoretical pI: 10.427
  • theoretical MW: 26915.2
  • GRAVY: -0.601339

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SACOL0028
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_IS240; DDE domain (PF13610; HMM-score: 131.5)
    and 10 more
    rve; Integrase core domain (PF00665; HMM-score: 56.9)
    NADP_Rossmann (CL0063) OYE-like_second_a-b; OYE-like second alpha/beta domain (PF22620; HMM-score: 18.9)
    HTH (CL0123) HTH_32; Homeodomain-like domain (PF13565; HMM-score: 17.2)
    RNase_H (CL0219) DDE_Tnp_IS66; Transposase IS66 family (PF03050; HMM-score: 15.5)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 15.5)
    Lag3_N (CL0720) Cauli_DNA-bind; Caulimovirus DNA-binding protein (PF03310; HMM-score: 13.6)
    HTH (CL0123) HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 13.4)
    no clan defined DUF1580; Protein of unknown function (DUF1580) (PF07618; HMM-score: 12.5)
    HEM4; Uroporphyrinogen-III synthase HemD (PF02602; HMM-score: 12.3)
    HTH (CL0123) HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.5605
    • Cytoplasmic Membrane Score: 0.0015
    • Cell wall & surface Score: 0.0042
    • Extracellular Score: 0.4338
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005085
    • TAT(Tat/SPI): 0.000236
    • LIPO(Sec/SPII): 0.001497
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHHSTVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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