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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00305 [old locus tag: SACOL0059 ]
  • pan locus tag?: SAUPAN000051000
  • symbol: SACOL_RS00305
  • pan gene symbol?: —
  • synonym:
  • product: dihydrouridine synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00305 [old locus tag: SACOL0059 ]
  • symbol: SACOL_RS00305
  • product: dihydrouridine synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 70485..70937
  • length: 453
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (70485..70937) NCBI
  • BioCyc: SACOL_RS00305 BioCyc
  • MicrobesOnline: see SACOL0059

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAGTAAAGTAGATGCACATTGGGAATTAATCGAAGCTATTAAAAATTTACGTGACGAA
    ATTGCACCAAACACATTGTTAACAATTAACGATGATATTCCCGATAGAAAAACAGGACTT
    GAACTTGCAGAAAAATATGGTATTGATGGCATCATGATTGGTAGAGGCATTTTCCACAAT
    CCATTCACTTTTGAAAAAGAACCACGCGAACACACAAGCAAAGAACTATTAGATTTGTTG
    AGATTGCATCTATCATTGTTTAACAAATATGAGAAAGATGAAATACGACAATTCAAGAGT
    TTGCGTAGATTCTTTAAAATCTATGTGCGTGGCATAAGAGGCGCTAGCGAACTTCGCCAT
    CAATTGATGAACACACAATCAATTGCAGAAGTACGAGCACTACTAGATGAATTTGAAGCC
    CAAATGGACGAAGACGTTAAAATTGAATTATAG
    60
    120
    180
    240
    300
    360
    420
    453

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS00305 [old locus tag: SACOL0059 ]
  • symbol: SACOL_RS00305
  • description: dihydrouridine synthase
  • length: 150
  • theoretical pI: 5.66706
  • theoretical MW: 17640.1
  • GRAVY: -0.483333

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 46.9)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 16.6)
  • TheSEED: see SACOL0059
  • PFAM:
    TIM_barrel (CL0036) Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 45.9)
    and 2 more
    no clan defined MPS2; Monopolar spindle protein 2 (PF17060; HMM-score: 14.9)
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001633
    • TAT(Tat/SPI): 0.000168
    • LIPO(Sec/SPII): 0.000236
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKVDAHWELIEAIKNLRDEIAPNTLLTINDDIPDRKTGLELAEKYGIDGIMIGRGIFHNPFTFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEVRALLDEFEAQMDEDVKIEL

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0059
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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