From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
  • pan locus tag?: SAUPAN000994000
  • symbol: SACOL_RS00765
  • pan gene symbol?: capP
  • synonym:
  • product: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
  • symbol: SACOL_RS00765
  • product: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
  • replicon: chromosome
  • strand: +
  • coordinates: 168828..169973
  • length: 1146
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (168828..169973) NCBI
  • BioCyc: SACOL_RS00765 BioCyc
  • MicrobesOnline: see SACOL0151

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAAAATTATGGTTATTTTCGGTACGAGACCCGAAGCAATAAAAATGGCACCATTA
    GTAAAAGAAATTGATCATAATGGGAACTTTGAAGCGAACATTGTGATTACAGCACAACAT
    AGAGATATGTTAGATAGTGTGTTAAGTATATTTGATATTCAAGCTGATCATGATTTAAAT
    ATTATGCAAGATCAACAAACATTAGCAGGCCTTACGGCGAATGCACTTGCTAAACTTGAT
    AGCATCATTAATGAGGAACAACCGGATATGATTTTAGTACATGGTGATACTACAACGACT
    TTTGTAGGAAGTTTGGCAGCATTTTATCATCAAATTCCGGTCGGACATGTAGAAGCTGGA
    CTTCGAACACATCAGAAATACTCACCATTTCCTGAAGAGTTAAATCGAGTCATGGTAAGT
    AATATTGCTGAATTGAATTTTGCGCCAACAGTAATTGCAGCTAAAAATTTACTTTTTGAA
    AACAAAGACAAAGAGCGTATCTTTATTACTGGAAATACAGTTATTGACGCATTGTCAACA
    ACAGTTCAAAATGATTTTGTTTCAACGATTATTAATAAACATAAAGGCAAGAAAGTTGTT
    TTACTAACAGCGCATCGTCGTGAAAATATTGGGGAACCGATGCATCAGATTTTTAAAGCA
    GTAAGAGATTTGGCAGATGAATATAAAGATGTTGTCTTCATTTATCCAATGCATCGTAAT
    CCAAAGGTAAGAGCGATTGCCGAAAAATATTTATCTGGGAGAAATCGGATTGAATTAATT
    GAGCCATTAGATGCGATTGAGTTCCATAATTTTACAAATCAATCGTACCTCGTGCTGACA
    GATTCTGGTGGTATTCAAGAGGAGGCTCCTACATTTGGAAAACCTGTGTTGGTATTAAGG
    AATCATACAGAGCGTCCCGAAGGCGTTGAGGCGGGAACATCGAGAGTAATTGGCACAGAT
    TATGACAATATTGTTCGAAATGTGAAACAATTGATTGAGGATGATGAAGCGTATCAACGT
    ATGAGTCAAGCGAATAATCCATATGGTGATGGACAAGCATCACGACGTATTTGTGAAGCA
    ATAGAATATTATTTTGGATTGCGCACAGACAAGCCGGATGAATTCGTACCTTTACGTCAC
    AAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
  • symbol: SACOL_RS00765
  • description: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
  • length: 381
  • theoretical pI: 6.02477
  • theoretical MW: 43091.7
  • GRAVY: -0.330446

Function[edit | edit source]

  • reaction:
    EC 5.1.3.14?  ExPASy
    UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 515.7)
    and 2 more
    UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 119.3)
    conserved hypothetical protein (TIGR03492; HMM-score: 24.3)
  • TheSEED: see SACOL0151
  • PFAM:
    GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 402)
    and 4 more
    Glyphos_transf; CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase (PF04464; HMM-score: 19.1)
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 18.3)
    LpxB; Lipid-A-disaccharide synthetase (PF02684; HMM-score: 13.3)
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 13.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00427
    • TAT(Tat/SPI): 0.000064
    • LIPO(Sec/SPII): 0.000408
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0151
  • protein localization: see SACOL0151
  • quantitative data / protein copy number per cell: see SACOL0151
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]