PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01100 [old locus tag: SACOL0215 ]
• pan locus tag^?: SAUPAN001096000
• symbol: SACOL_RS01100
• pan gene symbol^?: fadX
• synonym:
• product: acyl CoA:acetate/3-ketoacid CoA transferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01100 [old locus tag: SACOL0215 ]
• symbol: SACOL_RS01100
• product: acyl CoA:acetate/3-ketoacid CoA transferase
• replicon: chromosome
• strand: -
• coordinates: 254673..256250
• length: 1578
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (254673..256250) NCBI
• BioCyc: SACOL_RS01100 BioCyc
• MicrobesOnline: see SACOL0215

⊟Phenotype

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⊟DNA sequence

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  ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
  GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
  GCTGTGTTAGCGCAACATGACACATATCATACGCCCAATGATTTAACGTTTATATTAGCG
  AATGATATTCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
  ATTAAACGTGTCATTATGAGTATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
  AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
  AGTAATCAATTATTACCTGGAGTTATTACTAAAATCGGATTAAACACAGCTGTTGATCCT
  AGATACGGTGGCGGTAAAGTAAATACACGAACAACGGATGATTTAGTTTCATTAGTAACC
  ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
  GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
  TGTTATCATGTCGCATTAAACGCGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
  GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
  GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAAAGTCATTAT
  TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
  AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
  AGCATCGGTTATGGCATCAATAATGAACTCTCTAATTTATTGCATGAAGAATGTGTTGAA
  CATGATGTGCAACCGATTTTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
  CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
  ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
  AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGTGGCTTTATAGACATT
  ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
  TGCCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
  GTGAGCCATATCGACTTTAATGCACAATATTCACAATCACTCGAGCAAGAAATCTATTTT
  GTTACTGATCGTGCAGTATTCGAACTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
  CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
  GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
  TCAATTCATAAAGTATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS01100 [old locus tag: SACOL0215 ]
• symbol: SACOL_RS01100
• description: acyl CoA:acetate/3-ketoacid CoA transferase
• length: 525
• theoretical pI: 5.49984
• theoretical MW: 58876.2
• GRAVY: -0.163238

⊟Function

• TIGRFAM:
  3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 39.3)
  and 3 more
  3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
  Metabolism Energy metabolism Other malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.7)
• TheSEED: see SACOL0215
• PFAM:
  ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 71.4)
  and 3 more
  Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 22.5)
  AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 17.7)
  no clan defined Curlin_rpt; Curlin associated repeat (PF07012; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013776
  • TAT(Tat/SPI): 0.002257
  • LIPO(Sec/SPII): 0.000615
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SACOL0215
• RefSeq: WP_001789452 NCBI
• UniProt: see SACOL0215

⊟Protein sequence

• MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL0215

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications