From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
  • pan locus tag?: SAUPAN001898000
  • symbol: SACOL_RS02145
  • pan gene symbol?:
  • synonym:
  • product: acetyl-CoA acyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
  • symbol: SACOL_RS02145
  • product: acetyl-CoA acyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 429930..431111
  • length: 1182
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (429930..431111) NCBI
  • BioCyc: SACOL_RS02145 BioCyc
  • MicrobesOnline: see SACOL0426

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACGAGAGTCGTATTAGCAGCAGCATACAGGACACCTATTGGCGTTTTTGGAGGTGCG
    TTTAAAGACGTGCCAGCCTATGATTTAGGTGCGACTTTAATAGAACATATTATTAAAGAG
    ACGGGTTTGAATCCAAGTGAGATTGATGAAGTTATCATCGGTAACGTACTACAAGCAGGA
    CAAGGACAAAATCCAGCACGAATTGCTGCTATGAAAGGTGGCTTGCCAGAAACAGTACCT
    GCATTTACGGTGAATAAAGTATGTGGTTCTGGGTTAAAGTCGATTCAATTAGCATATCAA
    TCTATTGTGACTGGTGAAAATGACATCGTGCTAGCTGGCGGTATGGAGAATATGTCTCAA
    TCACCAATGCTTGTCAACAACAGTCGCTTTGGTTTTAAAATGGGACATCAATCAATGGTT
    GATAGCATGGTATATGATGGTTTAACAGATGTATTTAATCAATATCATATGGGTATTACT
    GCTGAAAATTTAGCAGAGCAATATGGTATTTCAAGAGAAGAACAAGATACATTTGCTGTA
    AACTCACAACAAAAAGCAGTACGTGCACAGCAAAATGGTGAATTTGATAGTGAAATAGTT
    CCAGTATCGATTCCTCAACGTAAAGGTGAACCAATCGTAGTCACTAAGGATGAAGGTGTA
    CGTGAAAATGTATCAGTCGAAAAATTAAGTCGATTAAGACCAGCTTTCAAAAAAGACGGT
    ACAGTTACAGCAGGTAATGCATCAGGAATCAATGATGGTGCTGCGATGATGTTAGTCATG
    TCAGAAGACAAAGCTAAAGAATTAAATATCGAACCATTGGCAGTGCTTGATGGCTTTGGA
    AGTCATGGTGTAGATCCTTCTATTATGGGTATTGCACCAGTTGGCGCTGTAGAAAAGGCT
    TTGAAACGTAGTAAAAAAGAATTAAGCGATATTGATGTATTTGAATTAAATGAAGCATTT
    GCAGCACAATCATTAGCTGTTGATCGTGAATTAAAATTACCTCCTGAAAAGGTGAATGTT
    AAAGGTGGCGCTATTGCATTAGGACATCCTATTGGTGCATCTGGTGCTAGAGTATTAGTG
    ACATTATTGCATCAACTGAATGATGAAGTTGAAACTGGTTTAACATCATTGTGTATTGGT
    GGCGGTCAAGCTATCGCTGCAGTTGTATCAAAGTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
  • symbol: SACOL_RS02145
  • description: acetyl-CoA acyltransferase
  • length: 393
  • theoretical pI: 4.94056
  • theoretical MW: 41675.3
  • GRAVY: -0.0450382

Function[edit | edit source]

  • reaction:
    EC 2.3.1.9?  ExPASy
    Acetyl-CoA C-acetyltransferase 2 acetyl-CoA = CoA + acetoacetyl-CoA
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 504.5)
    and 5 more
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 394.3)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 335.2)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 192.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 27.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 16.8)
  • TheSEED: see SACOL0426
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 365.8)
    and 4 more
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 151.3)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 28.1)
    ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 12.5)
    FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02427
    • TAT(Tat/SPI): 0.001086
    • LIPO(Sec/SPII): 0.0046
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLNPSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSIVTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGITAENLAEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDEGVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVLDGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLPPEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKYK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0426
  • protein localization: see SACOL0426
  • quantitative data / protein copy number per cell: see SACOL0426
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]