PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
• pan locus tag^?: SAUPAN001898000
• symbol: SACOL_RS02145
• pan gene symbol^?: —
• synonym:
• product: acetyl-CoA acyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
• symbol: SACOL_RS02145
• product: acetyl-CoA acyltransferase
• replicon: chromosome
• strand: +
• coordinates: 429930..431111
• length: 1182
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (429930..431111) NCBI
• BioCyc: SACOL_RS02145 BioCyc
• MicrobesOnline: see SACOL0426

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACGAGAGTCGTATTAGCAGCAGCATACAGGACACCTATTGGCGTTTTTGGAGGTGCG
  TTTAAAGACGTGCCAGCCTATGATTTAGGTGCGACTTTAATAGAACATATTATTAAAGAG
  ACGGGTTTGAATCCAAGTGAGATTGATGAAGTTATCATCGGTAACGTACTACAAGCAGGA
  CAAGGACAAAATCCAGCACGAATTGCTGCTATGAAAGGTGGCTTGCCAGAAACAGTACCT
  GCATTTACGGTGAATAAAGTATGTGGTTCTGGGTTAAAGTCGATTCAATTAGCATATCAA
  TCTATTGTGACTGGTGAAAATGACATCGTGCTAGCTGGCGGTATGGAGAATATGTCTCAA
  TCACCAATGCTTGTCAACAACAGTCGCTTTGGTTTTAAAATGGGACATCAATCAATGGTT
  GATAGCATGGTATATGATGGTTTAACAGATGTATTTAATCAATATCATATGGGTATTACT
  GCTGAAAATTTAGCAGAGCAATATGGTATTTCAAGAGAAGAACAAGATACATTTGCTGTA
  AACTCACAACAAAAAGCAGTACGTGCACAGCAAAATGGTGAATTTGATAGTGAAATAGTT
  CCAGTATCGATTCCTCAACGTAAAGGTGAACCAATCGTAGTCACTAAGGATGAAGGTGTA
  CGTGAAAATGTATCAGTCGAAAAATTAAGTCGATTAAGACCAGCTTTCAAAAAAGACGGT
  ACAGTTACAGCAGGTAATGCATCAGGAATCAATGATGGTGCTGCGATGATGTTAGTCATG
  TCAGAAGACAAAGCTAAAGAATTAAATATCGAACCATTGGCAGTGCTTGATGGCTTTGGA
  AGTCATGGTGTAGATCCTTCTATTATGGGTATTGCACCAGTTGGCGCTGTAGAAAAGGCT
  TTGAAACGTAGTAAAAAAGAATTAAGCGATATTGATGTATTTGAATTAAATGAAGCATTT
  GCAGCACAATCATTAGCTGTTGATCGTGAATTAAAATTACCTCCTGAAAAGGTGAATGTT
  AAAGGTGGCGCTATTGCATTAGGACATCCTATTGGTGCATCTGGTGCTAGAGTATTAGTG
  ACATTATTGCATCAACTGAATGATGAAGTTGAAACTGGTTTAACATCATTGTGTATTGGT
  GGCGGTCAAGCTATCGCTGCAGTTGTATCAAAGTATAAATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS02145 [old locus tag: SACOL0426 ]
• symbol: SACOL_RS02145
• description: acetyl-CoA acyltransferase
• length: 393
• theoretical pI: 4.94056
• theoretical MW: 41675.3
• GRAVY: -0.0450382

⊟Function

• reaction:
  EC 2.3.1.9?  ExPASy
  Acetyl-CoA C-acetyltransferase 2 acetyl-CoA = CoA + acetoacetyl-CoA
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 504.5)
  and 5 more
  3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 394.3)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 335.2)
  Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 192.8)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 27.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 16.8)
• TheSEED: see SACOL0426
• PFAM:
  Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 365.8)
  and 4 more
  Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 151.3)
  ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 28.1)
  ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 12.5)
  FAE1_CUT1_RppA; FAE1/Type III polyketide synthase-like protein (PF08392; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02427
  • TAT(Tat/SPI): 0.001086
  • LIPO(Sec/SPII): 0.0046
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446121210 NCBI
• RefSeq: WP_000199065 NCBI
• UniProt: see SACOL0426

⊟Protein sequence

• MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLNPSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSIVTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGITAENLAEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDEGVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVLDGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLPPEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKYK

⊟Experimental data

• experimentally validated: see SACOL0426
• protein localization: see SACOL0426
• quantitative data / protein copy number per cell: see SACOL0426
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications