From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02155 [old locus tag: SACOL0428 ]
  • pan locus tag?: SAUPAN001900000
  • symbol: SACOL_RS02155
  • pan gene symbol?: metE
  • synonym:
  • product: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS02155 [old locus tag: SACOL0428 ]
  • symbol: SACOL_RS02155
  • product: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 431989..434217
  • length: 2229
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (431989..434217) NCBI
  • BioCyc: SACOL_RS02155 BioCyc
  • MicrobesOnline: see SACOL0428

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGACAACAATTAAAACATCAAACTTAGGATTCCCAAGATTAGGTAGAAAAAGAGAATGG
    AAAAAAGCCATCGAAAGTTATTGGGCAAAGAAGATTTCTAAAGAAGAATTAGATCAAACA
    TTAACTGATCTACATAAAGAAAATTTATTATTACAAAAGTACTATCACTTAGATAGCATC
    CCAGTTGGTGATTTCTCTTTATATGACCATATATTAGATACGTCATTATTATTCAACATC
    ATCCCTGAACGTTTCCAAGGAAGAACTATTGATGATGATTTATTATTTGATATTGCTCGC
    GGTAATAAAGACCATGTTGCAAGTGCACTTATAAAATGGTTTAACACTAACTATCATTAC
    ATCGTACCTGAATGGGACAATGTTGAGCCTAAAGTGAGTCGCAATGTATTATTAGATCGC
    TTTAAATATGCCCAATCTTTAAACGTTAACGCTCACCCTGTCATTGTTGGTCCGATTACT
    TTTGTTAAATTATCAAAAGGCGGTCACCAAACTTTTGAAGAAAAAGTTAAAACGTTATTA
    CCACTTTATAAAGAAGTGTTTGAATCATTAATTGATGCAGGTGCTGAGTACATTCAAGTT
    GATGAGCCAATCTTAGTTACAGACGACAGCGAAAGCTATGAAAATATTACACGTGAAGCT
    TATGACTATTTCGAAAAAGCTGGTGTTGCTAAAAAATTAGTCATTCAAACATACTTTGAA
    CGTGCACATCTTAAATTCTTAAGTTCTTTACCTGTTGGTGGTATAGGTTTAGACTTTGTC
    CATGATAATGGCTATAACCTTAAACAAATTGAAGCTGGAGATTTTGATAAATCAAAAACA
    TTATACGCTGGAATTATTGATGGTCGTAATGTATGGGCAAGTGACATTGAAGCTAAAAAA
    GTCTTAATCGATAAATTGTTAGCACACACTAATGAACTTGTCATTCAACCATCATCTTCA
    TTATTACATGTTCCAGTATCTTTAGATGATGAAACATTAGATACAAGTGTTGGAGAAGGC
    TTAAGCTTTGCAACTGAAAAATTAGACGAATTAGATGCATTGCGTCGCCTATTTAATCAA
    AACGATAGTGTTAAATATGATAAATTAAAAGCACGTTATGAGCGTTTCCAAAATCAATCA
    TTCAAAAACTTAGATTATGATTTCGAAAGCGTGCGTACTTCAAGACAATCACCATTCGCG
    CAACGTATTGAACAACAACAAAAACGTTTGAACTTACCGGATTTACCAACAACAACTATT
    GGATCATTCCCACAAAGCCGAGAAGTTCGAAAATACCGTGCAGATTGGAAGAACAAACGC
    ATTACAGACGAAGCATATGAAACATTCTTAAAAAATGAAATTGCTCGATGGATTAAAATT
    CAAGAAGACATTGGCTTAGATGTATTAGTTCACGGTGAATTTGAACGTAATGACATGGTT
    GAATTCTTCGGAGAAAAATTACAAGGTTTCTTAGTAACTAAATTCGGTTGGGTGCAATCA
    TATGGTTCACGTGCCGTAAAACCACCAATCATTTATGGTGATGTAAAATGGACAGCGCCT
    TTAACTGTTGATGAAACAGTTTATGCACAAAGCTTAACAGATAAACCAGTTAAAGGTATG
    TTAACTGGACCTGTAACAATTCTAAACTGGTCATTTGAACGTGTTGATTTACCACGTAAA
    GTCGTTCAAGATCAAATTGCTTTAGCAATCAACGAAGAAGTATTAGCACTTGAAGCTGCA
    GGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCT
    GAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCA
    GTTCGTGATGAAACTCAAATCCATACACATATGTGTTATTCTCAATTCGGTCAAATCATT
    CATGCTATTCATGACTTAGATGCTGATGTTATTTCAATTGAAACATCTCGTAGCCATGGT
    GATTTAATTAAAGACTTTGAAGATATTAATTATGATTTAGGTATTGGTCTAGGTGTATAT
    GATATTCATAGTCCACGTATTCCAACAAAAGAAGAAATTACTACAGCAATCAATCGTTCA
    CTACAACAAATCGACCGCTCATTATTCTGGGTAAACCCTGACTGTGGTTTAAAAACGCGA
    AAAGAAGAAGAAGTTAAAGATGCATTGACTGTGCTTGTGAATGCTGTTAAAGCTAAACGC
    CAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2229

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS02155 [old locus tag: SACOL0428 ]
  • symbol: SACOL_RS02155
  • description: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
  • length: 742
  • theoretical pI: 5.40741
  • theoretical MW: 85074.8
  • GRAVY: -0.39434

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.14?  ExPASy
    5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase (TIGR01371; EC 2.1.1.14; HMM-score: 1093.7)
    and 1 more
    conserved hypothetical protein (TIGR04540; HMM-score: 14.2)
  • TheSEED: see SACOL0428
  • PFAM:
    Methionine_synt (CL0160) Meth_synt_2; Cobalamin-independent synthase, Catalytic domain (PF01717; HMM-score: 473.3)
    Meth_synt_1; Cobalamin-independent synthase, N-terminal domain (PF08267; HMM-score: 389.1)
    and 2 more
    URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 20)
    T3SS-Chaperone (CL0419) FliJ; Flagellar FliJ protein (PF02050; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002957
    • TAT(Tat/SPI): 0.000183
    • LIPO(Sec/SPII): 0.000535
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKRQE

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0428
  • protein localization: see SACOL0428
  • quantitative data / protein copy number per cell: see SACOL0428
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL_RS02155&oldid=88809"