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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03215 [old locus tag: SACOL0622 ]
  • pan locus tag?: SAUPAN002353000
  • symbol: SACOL_RS03215
  • pan gene symbol?: vraB
  • synonym:
  • product: acetyl-CoA acetyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03215 [old locus tag: SACOL0622 ]
  • symbol: SACOL_RS03215
  • product: acetyl-CoA acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 656124..657263
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (656124..657263) NCBI
  • BioCyc: SACOL_RS03215 BioCyc
  • MicrobesOnline: see SACOL0622

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACT
    TTAAAACATTTAGAGCCAGAACAATTGCTTAAACCTTTATTCCAACATTTTAAAGAGAAG
    TATCCAGAGGTAATATCTAAAATAGATGATGTAGTTTTAGGTAATGTTGTTGGGAATGGT
    GGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
    ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATGCATGTCGCATGATC
    CAAGCCGGAGCTGGCAAGGTATATATTGCAGGTGGTGTTGAAAGTACAAGTCGAGCACCT
    TGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCATTACCTGAGTTTTATGAGCGT
    GCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGTGGCC
    AAGATGTATGATGTTTCAAGAGGATTACAAGATGAATTTGCTTATCGAAGTCATCAATTG
    ACAGCGGAAAATGTAAAGAATGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAA
    GGAGAAATATTCAACACTGATGAAAGTCTAAAATCACATATTCCGAAAGATAACTTTGGC
    CGATTTAAGCCCGTGATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
    GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGGTTTCGAG
    CATGGTTTATTATTTAAAGATGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATT
    GGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATCAATTAACGATAGAAAATATT
    GAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTAGTTGCCTGCCAACAAGCTTTA
    AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATAC
    GGTGCAAGCGGTGCCCAATTAGTGACTCGATTATTTTATATGTTTGACAAAGAGACTATG
    ATTGCATCTATGGGGATAGGGGGAGGTCTAGGAAATGCAGCATTATTTACTCGATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03215 [old locus tag: SACOL0622 ]
  • symbol: SACOL_RS03215
  • description: acetyl-CoA acetyltransferase
  • length: 379
  • theoretical pI: 6.87843
  • theoretical MW: 41021.7
  • GRAVY: -0.0875989

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.-?  ExPASy
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Other acetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 380.9)
    and 4 more
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 259.1)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 208.4)
    Metabolism Fatty acid and phospholipid metabolism Degradation acetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 124.4)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis beta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 15.9)
  • TheSEED: see SACOL0622
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 188.2)
    and 2 more
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 104.2)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 19.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004423
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.000526
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRGLQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: see SACOL0622
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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